Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00931
(704 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 145 6e-36
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 89 6e-19
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 87 2e-18
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 85 1e-17
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 83 4e-17
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 77 2e-15
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 65 8e-12
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 65 8e-12
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 60 4e-10
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 59 5e-10
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 58 2e-09
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 54 2e-08
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 31 0.12
SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ... 29 0.49
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.6
SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyc... 26 6.0
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 25 8.0
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 145 bits (351), Expect = 6e-36
Identities = 59/85 (69%), Positives = 71/85 (83%)
Frame = +2
Query: 257 ICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPVL 436
I PDEG+Y+GGKF F ++ NYPH+PPKVKC +YHPNID+EGNVCLNILR+DW PVL
Sbjct: 58 IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVL 117
Query: 437 TVNSIVYGLQYLFLEPNPEDPLNKK 511
+NSI+ GLQ+LFL PN EDPLNK+
Sbjct: 118 NLNSILVGLQFLFLSPNAEDPLNKE 142
Score = 39.9 bits (89), Expect = 3e-04
Identities = 20/54 (37%), Positives = 33/54 (61%)
Frame = +3
Query: 69 MIKLFSLKQQKKDEEGSTRGGGSQKKASAAQLRITKDLNELNLPKTCNTEFPDP 230
M K++ LK+++ +E + G S AQ+RI KD+ +L +P T +T +PDP
Sbjct: 1 MRKIWELKKKEAQKEKNASG------ISPAQIRIQKDVTDLEIPSTMSTSWPDP 48
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 89.0 bits (211), Expect = 6e-19
Identities = 35/83 (42%), Positives = 53/83 (63%)
Frame = +2
Query: 254 VICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPV 433
++ P + Y GG F S +YP +PPKV T +YHPNI+ G++CL+ILR+ W P
Sbjct: 37 IMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPA 96
Query: 434 LTVNSIVYGLQYLFLEPNPEDPL 502
LT++ ++ + L +PNP+DPL
Sbjct: 97 LTISKVLLSICSLLTDPNPDDPL 119
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 87.4 bits (207), Expect = 2e-18
Identities = 38/81 (46%), Positives = 53/81 (65%), Gaps = 1/81 (1%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILRED-WKPVLT 439
P + Y GGKF FS K +YP +PP ++ T +YHPN D EGNVCL IL++ +KP +
Sbjct: 46 PSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIK 105
Query: 440 VNSIVYGLQYLFLEPNPEDPL 502
+ S++ + L EPNP+DPL
Sbjct: 106 LRSVLEQILQLLREPNPDDPL 126
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 84.6 bits (200), Expect = 1e-17
Identities = 33/81 (40%), Positives = 49/81 (60%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTV 442
P + Y GGKF + YP PP V+ T +YHPN+D G +CL+ L++DW P L +
Sbjct: 41 PQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQI 100
Query: 443 NSIVYGLQYLFLEPNPEDPLN 505
+++ +Q L PNP+DPL+
Sbjct: 101 RTVLLSIQALMGAPNPDDPLD 121
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 83.0 bits (196), Expect = 4e-17
Identities = 34/81 (41%), Positives = 49/81 (60%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTV 442
P + +Y G KF S NYP+ PP + + ++HPN+D+ GN+CL+IL++ W V V
Sbjct: 68 PSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNV 127
Query: 443 NSIVYGLQYLFLEPNPEDPLN 505
+I+ LQ L EPN PLN
Sbjct: 128 QTILLSLQSLLGEPNNASPLN 148
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 77.0 bits (181), Expect = 2e-15
Identities = 32/80 (40%), Positives = 48/80 (60%), Gaps = 1/80 (1%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLE-GNVCLNILREDWKPVLT 439
P+ Y GG FV ++ +YP PPK+ +T +YHPN+ + G +CL+IL++ W PV T
Sbjct: 43 PEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYT 102
Query: 440 VNSIVYGLQYLFLEPNPEDP 499
+ S + LQ L P P +P
Sbjct: 103 MKSALISLQSLLCTPEPSNP 122
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 65.3 bits (152), Expect = 8e-12
Identities = 30/86 (34%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
Frame = +2
Query: 278 YRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILRED--WKPVLTVNSI 451
+ GG + + YP PPK + ++HPN+ G VCL+IL E+ WKP +T+ I
Sbjct: 53 WEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQI 112
Query: 452 VYGLQYLFLEPNPEDPLNKKQRTSSK 529
+ G+Q L +PN P + T K
Sbjct: 113 LLGIQDLLDDPNIASPAQTEAYTMFK 138
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 65.3 bits (152), Expect = 8e-12
Identities = 28/85 (32%), Positives = 45/85 (52%)
Frame = +2
Query: 251 IVICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKP 430
++I P + + G F YP++PP VK + ++HPN+ G +CL+IL+ W P
Sbjct: 39 VIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSP 98
Query: 431 VLTVNSIVYGLQYLFLEPNPEDPLN 505
V +I+ +Q L +PN P N
Sbjct: 99 TYDVAAILTSIQSLLNDPNNASPAN 123
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 59.7 bits (138), Expect = 4e-10
Identities = 29/82 (35%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLNILREDWKPVLT 439
P E Y GG++V V YP PP V +T + HPNI G VC++IL+ W P +
Sbjct: 44 PTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWS 103
Query: 440 VNSIVYGLQYLFLEPNPEDPLN 505
+ S + L + PLN
Sbjct: 104 LQSACLAIISLLSNYDASSPLN 125
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 59.3 bits (137), Expect = 5e-10
Identities = 29/83 (34%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLNILREDWKPVL- 436
P E Y GG + ++ YP + P + ++HPNID L G+VCL+++ + W P+
Sbjct: 39 PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFD 98
Query: 437 TVNSIVYGLQYLFLEPNPEDPLN 505
+N L L PN DPLN
Sbjct: 99 MINIFEVFLPQLLRYPNASDPLN 121
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 57.6 bits (133), Expect = 2e-09
Identities = 31/98 (31%), Positives = 48/98 (48%), Gaps = 13/98 (13%)
Frame = +2
Query: 251 IVICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR----- 415
++I P++ Y GG F + +YP PPK+K T ++HPN+ G VC++IL
Sbjct: 41 MIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDD 100
Query: 416 --------EDWKPVLTVNSIVYGLQYLFLEPNPEDPLN 505
E W PV + +I+ + + PN E P N
Sbjct: 101 KYGYEDAGERWLPVHSPETILISVISMLSSPNDESPAN 138
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 54.0 bits (124), Expect = 2e-08
Identities = 30/94 (31%), Positives = 44/94 (46%), Gaps = 13/94 (13%)
Frame = +2
Query: 263 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR--------- 415
PD + GG + + K +YP PP +K E +HPN+ +G VC++IL
Sbjct: 45 PDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMY 104
Query: 416 ----EDWKPVLTVNSIVYGLQYLFLEPNPEDPLN 505
E W PV +V I+ + + EPN E N
Sbjct: 105 ESSSERWSPVQSVEKILLSVMSMLAEPNDESGAN 138
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 31.5 bits (68), Expect = 0.12
Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
Frame = +2
Query: 251 IVICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNIL---RED 421
I+ P + Y GG++ + P+YP +PP ++ T +CL+ +
Sbjct: 40 IITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSFSDFHPKS 97
Query: 422 WKPVLTVNSIVYGL 463
W P V++I+ GL
Sbjct: 98 WNPSWMVSTILVGL 111
>SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 29.5 bits (63), Expect = 0.49
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +1
Query: 73 LNFFH*NNRKKMKRVLPEEEDHKKKHQQP 159
+ +F NN K ++R+L E ED KH +P
Sbjct: 235 IRYFKHNNMKDLERILQELEDDFVKHNRP 263
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = +3
Query: 501 STRSSGRAPKQQATFRTTRSESYARRLRRHI 593
STRSS +P+Q + +RS+ A ++RHI
Sbjct: 2610 STRSSLNSPRQFWAYHGSRSKKIADIVKRHI 2640
>SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 585
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +1
Query: 469 FILGTKP*RSPQQEAADELQSNRRLFEQHVQRAMRGGYVGT 591
FI G K ++ Q A L+ RRL +H+Q GG +GT
Sbjct: 61 FINGFKIEKNQQDSAGQGLK--RRLKSRHIQMIGIGGAIGT 99
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.4 bits (53), Expect = 8.0
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +2
Query: 476 LEPNPEDPLNKKQRTSSKATGDFSNNTFRELCEAATSAHLISNDASNEN 622
L P PE+ + +R +SN+ L T HL+ N +S +N
Sbjct: 530 LVPYPENLIASIERLIPFIFSSYSNSQSVPLLPCPTQRHLLFNTSSTDN 578
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,701,822
Number of Sequences: 5004
Number of extensions: 58071
Number of successful extensions: 222
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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