Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00904
(767 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 155 9e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 155 9e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 155 9e-39
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 53 5e-08
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 52 1e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 47 3e-06
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 32 0.10
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 28 1.3
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 27 3.0
SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schiz... 26 5.2
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 25 9.0
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.0
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 9.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 155 bits (375), Expect = 9e-39
Identities = 67/88 (76%), Positives = 79/88 (89%)
Frame = +1
Query: 256 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 435
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360
Query: 436 CHTAHIACKFAEIKEKVDRRTGKSMKST 519
CHTAHIACKFAE+ EK+DRR+GK ++ +
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEES 388
Score = 143 bits (346), Expect = 3e-35
Identities = 66/81 (81%), Positives = 72/81 (88%)
Frame = +2
Query: 5 KEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 184
K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV
Sbjct: 217 KAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIV 276
Query: 185 VFAPANITTEVKSVEMHHEAL 247
FAPA +TTEVKSVEMHHE+L
Sbjct: 277 TFAPAGVTTEVKSVEMHHESL 297
Score = 97.5 bits (232), Expect = 2e-21
Identities = 46/70 (65%), Positives = 54/70 (77%)
Frame = +3
Query: 510 EVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAG 689
E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G
Sbjct: 386 EESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPG 445
Query: 690 GGKVTKSCRK 719
KVTK+ K
Sbjct: 446 AAKVTKAAVK 455
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 155 bits (375), Expect = 9e-39
Identities = 67/88 (76%), Positives = 79/88 (89%)
Frame = +1
Query: 256 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 435
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360
Query: 436 CHTAHIACKFAEIKEKVDRRTGKSMKST 519
CHTAHIACKFAE+ EK+DRR+GK ++ +
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEES 388
Score = 143 bits (346), Expect = 3e-35
Identities = 66/81 (81%), Positives = 72/81 (88%)
Frame = +2
Query: 5 KEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 184
K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV
Sbjct: 217 KAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIV 276
Query: 185 VFAPANITTEVKSVEMHHEAL 247
FAPA +TTEVKSVEMHHE+L
Sbjct: 277 TFAPAGVTTEVKSVEMHHESL 297
Score = 97.5 bits (232), Expect = 2e-21
Identities = 46/70 (65%), Positives = 54/70 (77%)
Frame = +3
Query: 510 EVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAG 689
E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G
Sbjct: 386 EESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPG 445
Query: 690 GGKVTKSCRK 719
KVTK+ K
Sbjct: 446 AAKVTKAAVK 455
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 155 bits (375), Expect = 9e-39
Identities = 67/88 (76%), Positives = 79/88 (89%)
Frame = +1
Query: 256 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 435
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360
Query: 436 CHTAHIACKFAEIKEKVDRRTGKSMKST 519
CHTAHIACKFAE+ EK+DRR+GK ++ +
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEES 388
Score = 143 bits (346), Expect = 3e-35
Identities = 66/81 (81%), Positives = 72/81 (88%)
Frame = +2
Query: 5 KEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 184
K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV
Sbjct: 217 KAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIV 276
Query: 185 VFAPANITTEVKSVEMHHEAL 247
FAPA +TTEVKSVEMHHE+L
Sbjct: 277 TFAPAGVTTEVKSVEMHHESL 297
Score = 97.5 bits (232), Expect = 2e-21
Identities = 46/70 (65%), Positives = 54/70 (77%)
Frame = +3
Query: 510 EVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAG 689
E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G
Sbjct: 386 EESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPG 445
Query: 690 GGKVTKSCRK 719
KVTK+ K
Sbjct: 446 AAKVTKAAVK 455
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 52.8 bits (121), Expect = 5e-08
Identities = 30/75 (40%), Positives = 44/75 (58%), Gaps = 3/75 (4%)
Frame = +2
Query: 32 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPAN 202
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V ++
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 203 ITTEVKSVEMHHEAL 247
+ T V +EM + L
Sbjct: 294 LKTTVTGIEMFKKQL 308
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 51.6 bits (118), Expect = 1e-07
Identities = 31/98 (31%), Positives = 48/98 (48%)
Frame = +1
Query: 220 ICGDAPRSSPRSVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHP 399
I +A S+ GD V V+ +++ GYV +KN P F AQ+ +L P
Sbjct: 513 IYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELP 570
Query: 400 GQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSMK 513
++ GY+ V+ HTA FA++ K+D+ KS K
Sbjct: 571 SILTTGYSCVMHIHTAVEEVSFAKLLHKLDKTNRKSKK 608
Score = 43.6 bits (98), Expect = 3e-05
Identities = 25/78 (32%), Positives = 43/78 (55%)
Frame = +2
Query: 23 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 202
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N
Sbjct: 448 GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPIN 505
Query: 203 ITTEVKSVEMHHEALQEV 256
T EV ++ + EA +E+
Sbjct: 506 QTLEVTAI--YDEADEEI 521
Score = 40.7 bits (91), Expect = 2e-04
Identities = 20/53 (37%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Frame = +3
Query: 519 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 674
P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 610 PMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 46.8 bits (106), Expect = 3e-06
Identities = 26/68 (38%), Positives = 38/68 (55%)
Frame = +2
Query: 23 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 202
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ +
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQ 442
Query: 203 ITTEVKSV 226
VK+V
Sbjct: 443 EDAYVKNV 450
Score = 31.9 bits (69), Expect = 0.10
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +3
Query: 573 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 665
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 31.9 bits (69), Expect = 0.10
Identities = 24/84 (28%), Positives = 37/84 (44%)
Frame = +2
Query: 5 KEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 184
K GK + L E + I PP + PLR L D + G + + R+ G +K G V
Sbjct: 217 KTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRIMEGFIKKGGKV 276
Query: 185 VFAPANITTEVKSVEMHHEALQEV 256
+ EV+ V + + + EV
Sbjct: 277 MSVNTGRKYEVQQVGIMYPDMTEV 300
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 28.3 bits (60), Expect = 1.3
Identities = 22/63 (34%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
Frame = -3
Query: 282 VETYIVSRYTSWRASWCISTDL--TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILY 109
V+T +S+ T AS S L +SVV+ + +T V + + VST TGTV +P
Sbjct: 63 VQTIAISQ-TDNAASCIPSASLLSSSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSS 121
Query: 108 TSC 100
+C
Sbjct: 122 VAC 124
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 27.1 bits (57), Expect = 3.0
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 1/72 (1%)
Frame = -3
Query: 336 GVTSNITTTQFLDGHVLYVETYIVSRYTSWRASWCISTDLTSVVMLAGAKTTMVPGF-NT 160
G SN T+ +D + T + YT+ W ST LTS+V +G++T + T
Sbjct: 688 GSGSNSIVTETVD---VPTTTSVNYGYTTITTGWTGSTTLTSIVTHSGSETGPTTVYIET 744
Query: 159 PVSTLPTGTVPI 124
P + T T I
Sbjct: 745 PSVSATTTTTTI 756
>SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 355
Score = 26.2 bits (55), Expect = 5.2
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2
Query: 766 KIVLLTALATSCPWWPFRQLLVTLPP 689
K +++ ALA C W+ +LL TL P
Sbjct: 10 KEIVVYALAFGCSWYAAHKLLSTLDP 35
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 25.4 bits (53), Expect = 9.0
Identities = 14/53 (26%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -3
Query: 405 LTRMVKHNDL-SCKICSTLRWVVFGVTSNITTTQFLDGHVLYVETYIVSRYTS 250
L R++KH + S + L W+V +T T+ F ++ Y+ YT+
Sbjct: 317 LNRLLKHLESPSSSSVNPLVWIVLLLTGPFLTSLFTQFYLFMSTRYLARSYTT 369
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.0
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 165 NTPVSTLPTGTVPIPPIL 112
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.4 bits (53), Expect = 9.0
Identities = 20/60 (33%), Positives = 23/60 (38%), Gaps = 5/60 (8%)
Frame = -3
Query: 174 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 10
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,259,039
Number of Sequences: 5004
Number of extensions: 72044
Number of successful extensions: 247
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -