BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00890
(724 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc... 29 0.89
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa... 28 1.2
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 27 2.7
SPCC16C4.01 |sif2|SPCC5E4.09|Sad1 interacting factor 2|Schizosac... 27 3.6
SPAC823.07 |||GPI-phospholipase A2 activity regulator |Schizosac... 26 4.7
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 26 6.3
SPAC186.09 |||pyruvate decarboxylase |Schizosaccharomyces pombe|... 25 8.3
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 25 8.3
SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyc... 25 8.3
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch... 25 8.3
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 8.3
>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1141
Score = 28.7 bits (61), Expect = 0.89
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 5/60 (8%)
Frame = +3
Query: 387 GHSFTAYMHDPEH----RYLEQFGVIADEATTMRDPFFYRWHAYMMTFSRST-RNLPTCV 551
GH +T Y+HD E+ +Y +++ + DE+ D + YM+T+ R R++ CV
Sbjct: 1075 GHYWT-YIHDFENNVYRKYNDEYVTVVDESEIFADTTGNNANPYMLTYIRKEYRHIIECV 1133
>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 28.3 bits (60), Expect = 1.2
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = -2
Query: 681 SPRDKFTSLSIQYVFSVFGCPPGVSTDTDLTCTPWFSSS 565
SP+ F++L + + ++F C G+ + T PW +S
Sbjct: 169 SPKPLFSALQVYPLATIFNCVAGLPQGFESTVFPWNKTS 207
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 27.1 bits (57), Expect = 2.7
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 509 DDVFQKHKESAYVRPYTRSELENQGVQVRSVSVETPG 619
+ V KH E T++ELE + +R ++TPG
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPG 96
>SPCC16C4.01 |sif2|SPCC5E4.09|Sad1 interacting factor
2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 446
Score = 26.6 bits (56), Expect = 3.6
Identities = 17/71 (23%), Positives = 31/71 (43%), Gaps = 3/71 (4%)
Frame = +3
Query: 312 DTLGNMLESSALSPNRELYGSIHNNGHSFTA---YMHDPEHRYLEQFGVIADEATTMRDP 482
++L ++ E S L N+ L+ + + Y D EHR+L + G E + D
Sbjct: 137 ESLQSIFEESGLDRNQPLFREVFCFTYGVVVLWGYTIDEEHRFLRELGRFEIEKLKIEDM 196
Query: 483 FFYRWHAYMMT 515
++ Y+ T
Sbjct: 197 EVEEFNYYITT 207
>SPAC823.07 |||GPI-phospholipase A2 activity regulator
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 331
Score = 26.2 bits (55), Expect = 4.7
Identities = 17/44 (38%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = -3
Query: 542 RQIPCASGKRHHVGVPAVEEWIAHRC-GLISDNTKL-FKVSVFR 417
RQI ++G H V V V I ++C G SD +KL + +FR
Sbjct: 20 RQISASAGDLHPVYVSCVNRCIENKCHGNPSDTSKLPLDLKLFR 63
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 25.8 bits (54), Expect = 6.3
Identities = 14/29 (48%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = -1
Query: 595 PHLHALVLE-LRSGIRTHVGRFLVLLENV 512
P+L A + E L G++THVGR +LL+ V
Sbjct: 630 PYLRAKLAEILYFGVQTHVGRSELLLDVV 658
>SPAC186.09 |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 572
Score = 25.4 bits (53), Expect = 8.3
Identities = 13/51 (25%), Positives = 30/51 (58%)
Frame = +1
Query: 127 QFDFVTWMAPTSVRYAMAGPESCRRSLFVTIDEMERWRRNVEELSRLVPLD 279
Q+ + W P++ YA+ P +R++ V + + ++ V+E+S++V L+
Sbjct: 421 QWGHIGWSVPSAFGYAIGAP---KRNVVVFVGD-GSFQETVQEVSQMVRLN 467
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 139 RSQTVKLREVSLRNWLPPVAEFFNS 65
R K+ E+ RNW PPV E+ S
Sbjct: 441 RDARKKIIEIHTRNWDPPVPEWLCS 465
>SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1050
Score = 25.4 bits (53), Expect = 8.3
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +2
Query: 482 ILLPLARLHDDVFQKHKESAYVRPYTRSELEN 577
++L LA ++ D ++ ++ +VRP SELEN
Sbjct: 817 VVLCLAAMNLDAYRYIVKNEHVRPSKSSELEN 848
>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 775
Score = 25.4 bits (53), Expect = 8.3
Identities = 16/49 (32%), Positives = 21/49 (42%), Gaps = 1/49 (2%)
Frame = +3
Query: 264 TGTVRLPNGQTRPLDI-DTLGNMLESSALSPNRELYGSIHNNGHSFTAY 407
T L N D+ + L L ALS R+ YG NGH+ + Y
Sbjct: 164 TYVTNLDNATCSKCDLAENLWMCLTCGALSCGRKQYGGGGGNGHALSHY 212
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 127 QFDFVTWMAPTSVRYAMAGPESCRRSLFVTID 222
Q+ + W P+++ YA+A PE RR++ + D
Sbjct: 417 QWGHIGWSVPSAMGYAVAAPE--RRTIVMVGD 446
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,155,214
Number of Sequences: 5004
Number of extensions: 70332
Number of successful extensions: 239
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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