Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00886
(761 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 139 3e-34
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 139 3e-34
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 78 1e-15
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 62 1e-10
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 42 7e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 0.001
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 27 3.9
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 26 5.1
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 26 6.7
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 26 6.7
SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyce... 25 8.9
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 25 8.9
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 139 bits (337), Expect = 3e-34
Identities = 68/84 (80%), Positives = 73/84 (86%)
Frame = +2
Query: 509 TFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINVESGEHIV 688
T + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C ESGEHIV
Sbjct: 471 TSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIV 530
Query: 689 AGAGELHLEICLKDLEEDHACIPI 760
AGAGELHLEICLKDL+EDHA IP+
Sbjct: 531 AGAGELHLEICLKDLQEDHAGIPL 554
Score = 138 bits (334), Expect = 8e-34
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = +3
Query: 255 VPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAI 434
VPTSD+GRFYAFGRVFSG V +G K RI GPN+ PGKK+DL+ K IQRT+LMMG +E I
Sbjct: 386 VPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPI 445
Query: 435 EDVPSGNICGLVGVDQFLVKTGTITLSRM 521
ED P+GNI GLVGVDQFLVK+GT+T S +
Sbjct: 446 EDCPAGNIIGLVGVDQFLVKSGTLTTSEV 474
Score = 121 bits (291), Expect = 1e-28
Identities = 54/84 (64%), Positives = 69/84 (82%)
Frame = +1
Query: 4 KIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPH 183
K+ VTIK ++ + +GKALLKVVMR +LPA +AL++MI +HLPSP AQ+YR E LYEGP
Sbjct: 302 KLEVTIKPDEKELEGKALLKVVMRKFLPAADALMEMIVLHLPSPKTAQQYRAETLYEGPM 361
Query: 184 DDEAAIGIKSCDPEAPLMMYVSKM 255
DDE A+GI++CD APLM+YVSKM
Sbjct: 362 DDECAVGIRNCDANAPLMIYVSKM 385
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 139 bits (337), Expect = 3e-34
Identities = 68/84 (80%), Positives = 73/84 (86%)
Frame = +2
Query: 509 TFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINVESGEHIV 688
T + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C ESGEHIV
Sbjct: 471 TSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIV 530
Query: 689 AGAGELHLEICLKDLEEDHACIPI 760
AGAGELHLEICLKDL+EDHA IP+
Sbjct: 531 AGAGELHLEICLKDLQEDHAGIPL 554
Score = 138 bits (334), Expect = 8e-34
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = +3
Query: 255 VPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAI 434
VPTSD+GRFYAFGRVFSG V +G K RI GPN+ PGKK+DL+ K IQRT+LMMG +E I
Sbjct: 386 VPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPI 445
Query: 435 EDVPSGNICGLVGVDQFLVKTGTITLSRM 521
ED P+GNI GLVGVDQFLVK+GT+T S +
Sbjct: 446 EDCPAGNIIGLVGVDQFLVKSGTLTTSEV 474
Score = 121 bits (291), Expect = 1e-28
Identities = 54/84 (64%), Positives = 69/84 (82%)
Frame = +1
Query: 4 KIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPH 183
K+ VTIK ++ + +GKALLKVVMR +LPA +AL++MI +HLPSP AQ+YR E LYEGP
Sbjct: 302 KLEVTIKPDEKELEGKALLKVVMRKFLPAADALMEMIVLHLPSPKTAQQYRAETLYEGPM 361
Query: 184 DDEAAIGIKSCDPEAPLMMYVSKM 255
DDE A+GI++CD APLM+YVSKM
Sbjct: 362 DDECAVGIRNCDANAPLMIYVSKM 385
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 78.2 bits (184), Expect = 1e-15
Identities = 39/81 (48%), Positives = 52/81 (64%)
Frame = +2
Query: 518 NAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINVESGEHIVAGA 697
N N+ + + P+VRVA+EP P ++ KLV GL L ++DP VQ E+GEH++ A
Sbjct: 549 NGPNLVGVTQQMEPIVRVALEPVRPFEMNKLVTGLDMLNQADPCVQIAVEENGEHVIMCA 608
Query: 698 GELHLEICLKDLEEDHACIPI 760
GE+HLE CLKDL E A I I
Sbjct: 609 GEIHLERCLKDLRERFAKIEI 629
Score = 55.2 bits (127), Expect = 1e-08
Identities = 27/84 (32%), Positives = 46/84 (54%), Gaps = 1/84 (1%)
Frame = +3
Query: 267 DKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEK-TIQRTILMMGRYVEAIEDV 443
DK F R++SG + GQ+ + GP + P E K T++ LMMG+ + +E V
Sbjct: 464 DKDILIGFARIYSGTISVGQEVYVYGPKYDPVNPEKHITKVTVESLYLMMGQELVYLETV 523
Query: 444 PSGNICGLVGVDQFLVKTGTITLS 515
P+GN+ + G+ +++T T+ S
Sbjct: 524 PAGNVFAIGGLAGTVLRTATLCSS 547
Score = 50.8 bits (116), Expect = 2e-07
Identities = 30/93 (32%), Positives = 49/93 (52%), Gaps = 8/93 (8%)
Frame = +1
Query: 1 KKIGVTIKHED-SDKDGKALLKVVMRSWLPAGEALLQMIAIHLPSPVVAQKYR-MEMLYE 174
K + + + D KD + LL + + WLP A+L +PSP+ AQ R ++L
Sbjct: 309 KALNIKVLPRDIKSKDPRNLLLAIFQQWLPLSTAILLTAIREIPSPINAQANRARKVLSS 368
Query: 175 GPH----DDEAAIGIKSCD--PEAPLMMYVSKM 255
PH D + + ++SCD E P+++Y+SKM
Sbjct: 369 TPHYEMIDPDITLAMESCDASKEQPVLVYISKM 401
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 61.7 bits (143), Expect = 1e-10
Identities = 30/67 (44%), Positives = 45/67 (67%)
Frame = +2
Query: 560 VVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINVESGEHIVAGAGELHLEICLKDLEE 739
V +VAVEP NP++LPKL++GL++ KS P+ ESGEH + G GE++++ L DL
Sbjct: 600 VFKVAVEPHNPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRT 659
Query: 740 DHACIPI 760
++ I I
Sbjct: 660 LYSEIEI 666
Score = 58.8 bits (136), Expect = 8e-10
Identities = 30/82 (36%), Positives = 45/82 (54%)
Frame = +3
Query: 261 TSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIED 440
T D FYAF RV+SG+V GQK +++G N++ +ED+ I + RY ++
Sbjct: 497 TVDANNFYAFARVYSGQVKKGQKVKVLGENYSLEDEEDMVVAHIAEICVPCARYRLHVDG 556
Query: 441 VPSGNICGLVGVDQFLVKTGTI 506
+G + L GVD + KT TI
Sbjct: 557 AVAGMLVLLGGVDNSISKTATI 578
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 42.3 bits (95), Expect = 7e-05
Identities = 21/68 (30%), Positives = 40/68 (58%), Gaps = 1/68 (1%)
Frame = +2
Query: 545 FSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INVESGEHIVAGAGELHLEIC 721
F PV+ ++++PK+ D + L R + DP + ++ ES E I++G GELHLE+
Sbjct: 470 FVPEPVISLSLKPKSK-DTTSFSKALNRFQREDPTFRVQLDNESKETIISGMGELHLEVY 528
Query: 722 LKDLEEDH 745
++ + ++
Sbjct: 529 VERMRREY 536
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/60 (30%), Positives = 35/60 (58%), Gaps = 1/60 (1%)
Frame = +2
Query: 557 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCI-NVESGEHIVAGAGELHLEICLKDL 733
PV ++EP + D P L+E L + + DP + ++E+G+ ++ G G +HL++ + L
Sbjct: 443 PVCIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERL 502
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 26.6 bits (56), Expect = 3.9
Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = -3
Query: 705 SSPAPATMCSPDS--TLIHCTMGSDLARRLRPS 613
S+ +P+T+CSPD+ T ++ G L ++ P+
Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435
>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 241
Score = 26.2 bits (55), Expect = 5.1
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = +2
Query: 590 PADLPKLVEGLKRLAKSD--PMVQCIN 664
P D+ KL EGL++L + D +VQ +N
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVN 198
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 25.8 bits (54), Expect = 6.7
Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +3
Query: 342 GPNFTP-GKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVG 473
G +F P GK ++ + Q + + RY+ DVP+G+I G+ G
Sbjct: 115 GSDFDPKGKSDNEIRRFSQAFMRQLFRYIGPQTDVPAGDI-GVTG 158
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 577 NSDTHDW*YTEFHHLHVVGILESVI 503
N H W + E H ++GIL S+I
Sbjct: 721 NPHVHAWTHCEIHPAMILGILASII 745
>SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 351
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = +2
Query: 647 MVQCINVESGEHIVAGAGELHLEICLKDLEEDHACIPI 760
M + IN++S + +E CL L+E H +P+
Sbjct: 1 MKEMINLDSSSEPSIVTKQFSVEECLSKLKEQHCYVPM 38
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 25.4 bits (53), Expect = 8.9
Identities = 22/68 (32%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
Frame = -3
Query: 741 SSSRSLRQISRWSSPAPATMCSPDSTLIHCTMGSDLARRLRPSTS--LGRSAGFLGSTAT 568
SS S +S SS ++ S DST I T + L+P+TS + SA L S
Sbjct: 271 SSFTSSTPVSLTSSSTSSSGSSQDSTTIDSTPSTIATSTLQPTTSSPITTSAPSLSSALP 330
Query: 567 RTTGDTLN 544
T +L+
Sbjct: 331 TTYPSSLS 338
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,357,665
Number of Sequences: 5004
Number of extensions: 72595
Number of successful extensions: 249
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 246
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -