BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00791-2
(363 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 0.68
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 27 1.2
SPBC336.09c |rrn7||RNA polymerase I transcription factor subunit... 25 2.7
SPCC1672.02c |sap1||switch-activating protein Sap1|Schizosacchar... 25 3.6
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 25 3.6
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 24 6.3
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 24 6.3
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 24 8.3
SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 24 8.3
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 27.5 bits (58), Expect = 0.68
Identities = 15/58 (25%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +1
Query: 28 EDQLYFMTKTKGKPTRQSDADRNERRRNAIRELQNKTDIIQKIADL-FRHLQAEIIAE 198
+DQLY + TK K + ++ +EL+NK + Q ++D ++++ + I E
Sbjct: 1101 KDQLYQLQATKNKELEAKVKECLNNIKSLTKELENKEEKCQNLSDASLKYIELQEIHE 1158
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 26.6 bits (56), Expect = 1.2
Identities = 17/42 (40%), Positives = 24/42 (57%)
Frame = -1
Query: 213 IRRARFSYDFRL*MSE*ISNFLNYIRFVLKFSDGVPSTLIPV 88
IR+ SYDF + + E I NF+N VL +D V S+L +
Sbjct: 389 IRKLADSYDFAVVVDETIGNFVNV--EVLPLADVVVSSLTKI 428
>SPBC336.09c |rrn7||RNA polymerase I transcription factor subunit
Rrn7 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 537
Score = 25.4 bits (53), Expect = 2.7
Identities = 9/24 (37%), Positives = 20/24 (83%)
Frame = +2
Query: 65 NRLVNQMPTGMSVEGTPSENFKTK 136
+RL+N + + +S++GT S+N+++K
Sbjct: 294 SRLLNLVHSQISLQGTSSDNYQSK 317
>SPCC1672.02c |sap1||switch-activating protein
Sap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 3.6
Identities = 17/58 (29%), Positives = 26/58 (44%)
Frame = +1
Query: 4 EEFVATYIEDQLYFMTKTKGKPTRQSDADRNERRRNAIRELQNKTDIIQKIADLFRHL 177
+E+ A E Q Y GK + + NER + E QN ++ K+ L +HL
Sbjct: 117 DEWKARLSELQQYREKFLVGKRKCDCNDEINERLKKLTEEQQNVDMLVAKVNFLSKHL 174
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1919
Score = 25.0 bits (52), Expect = 3.6
Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -3
Query: 163 DQQFSE-LYPFCFEVL*WRSFDAHSGRHLIDESVYLW 56
+ +FSE Y F+ L W+S D L+ +Y+W
Sbjct: 1111 NHEFSESCYKRHFDRLIWQSLDTTPDNKLLKNILYIW 1147
>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 649
Score = 24.2 bits (50), Expect = 6.3
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -1
Query: 162 ISNFLNYIRFVLKFSDGVPSTLI 94
I NF NYI F L +D VP+ I
Sbjct: 125 IQNFWNYICFELLANDTVPANPI 147
>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1369
Score = 24.2 bits (50), Expect = 6.3
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = +1
Query: 13 VATYIEDQLYFMTKTKGKPTRQSDADRNERRRNAIRELQNKTDIIQK 153
+A I D L +K GKP + R+R L +TD++++
Sbjct: 977 IAAVIRDTLTIDSKMDGKPYKVGKFAHTLRKRLMREHLGLETDVLEQ 1023
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 23.8 bits (49), Expect = 8.3
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = +2
Query: 296 EADSQTIFRLDKDGNYKMKESP 361
+A T+ +L K G++K+KE+P
Sbjct: 127 QAHEPTLQKLAKFGSFKLKETP 148
>SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 507
Score = 23.8 bits (49), Expect = 8.3
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = +1
Query: 163 LFRHLQAEIIAETSAPNIISPYFRDIF 243
L RH A ++A + I PYF F
Sbjct: 18 LLRHCAARLVAFVNNTTISKPYFSTFF 44
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,499,386
Number of Sequences: 5004
Number of extensions: 28311
Number of successful extensions: 79
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 78
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 79
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 112046990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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