Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00676
(733 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24C9.08 |||vacuolar carboxypeptidase |Schizosaccharomyces po... 56 4e-09
SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos... 29 0.90
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 28 1.2
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 28 1.6
SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo... 27 3.6
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 26 4.8
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 26 4.8
SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyce... 26 6.4
SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharom... 25 8.4
>SPAC24C9.08 |||vacuolar carboxypeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 596
Score = 56.4 bits (130), Expect = 4e-09
Identities = 32/86 (37%), Positives = 48/86 (55%), Gaps = 3/86 (3%)
Frame = +1
Query: 256 ELPSILLNSHMDVVPVFENS---WKHPPFAAEIEDNVIYARGVQDMKSVGIQYIEAVRRL 426
+L ++L H DVVPV + S W PPF+A + +Y+RG D K+ + +EA+ L
Sbjct: 188 DLKPLVLMGHQDVVPVNQASLDRWYFPPFSATYHNGHVYSRGAADDKNSVVAILEALEIL 247
Query: 427 KETGVKLKRTVHLSFVPDEEIGGDTG 504
+ K ++TV SF DEE+ G G
Sbjct: 248 AISDYKPEQTVIASFGFDEEVSGYRG 273
>SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 891
Score = 28.7 bits (61), Expect = 0.90
Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = -1
Query: 235 HYQRRLLWQRFDHLHL*SNLFSFIFEEV--DAFIVVN 131
HY R++LW + H + +FSF EE D ++ VN
Sbjct: 238 HYCRKILWPSLHYQH--NEIFSFFHEESNWDDYVAVN 272
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +3
Query: 18 RFAL-FPQCSEYIQQVQSRLTPASRPYR-TILEYVQYIR 128
RF L +P+C + I + +L P S PY LE + Y+R
Sbjct: 378 RFRLAYPECFQIISSLCDKLGPHSDPYLIPALEVIDYLR 416
>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 27.9 bits (59), Expect = 1.6
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = +2
Query: 47 IHTTSSVKIDPSVSTLQNYLRIRSVHPNVDYNECINFLKNEAEKIG 184
+HT S ++ S+S L N I VH N + I+ ++G
Sbjct: 269 LHTVDSTNVNGSLSPLSNSSSINQVHQNETHGSTISVPNPNLSQMG 314
>SPBC8D2.05c |sfi1||spindle pole body protein
Sfi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 840
Score = 26.6 bits (56), Expect = 3.6
Identities = 18/64 (28%), Positives = 28/64 (43%), Gaps = 4/64 (6%)
Frame = +3
Query: 30 FPQCSEYIQQVQSRLTPASRPYRTILEYVQYIRTLTTMNASTSSKMKLKR----LDYKCK 197
FP+ + ++ +QSR+ A YR IL R K++ ++ LD K
Sbjct: 207 FPRWVQKLRNIQSRIYSAGEAYRLILIGQMVNRWRQKTRERLHQKLRFQKNAFLLDVLLK 266
Query: 198 WSNL 209
WS L
Sbjct: 267 WSAL 270
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/42 (28%), Positives = 18/42 (42%)
Frame = +2
Query: 593 PGLQWRKNNLACENHLSRKIRSRFPSAPRQLRRKVAVHNRQV 718
P L+ + CE + RK +R P R+K H Q+
Sbjct: 80 PSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQL 121
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/42 (28%), Positives = 18/42 (42%)
Frame = +2
Query: 593 PGLQWRKNNLACENHLSRKIRSRFPSAPRQLRRKVAVHNRQV 718
P L+ + CE + RK +R P R+K H Q+
Sbjct: 80 PSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQL 121
>SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 217
Score = 25.8 bits (54), Expect = 6.4
Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = +2
Query: 242 LGEQLSCRAYYST---LIWTWCLSSRTAGS 322
LG LS +YST + W W L+ RT G+
Sbjct: 120 LGRFLSWVPFYSTSKIVFWLWLLNPRTQGA 149
>SPBC3B9.09 |vps36||RBZ zinc finger protein
Vps36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 467
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +1
Query: 199 GRTFAKEADVGNDLVRRAAELPSILLNSHMDVVPVFENS 315
G T +++D L+ ++ +L +L +H+D VPV NS
Sbjct: 270 GLTGTQQSDKVQQLLNKSNQL-GVLRGNHLDNVPVSANS 307
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,104,788
Number of Sequences: 5004
Number of extensions: 65816
Number of successful extensions: 212
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 211
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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