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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00624X
         (509 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g50920.1 68414.m05725 GTP-binding protein-related similar to ...   127   5e-30
At1g10300.1 68414.m01160 GTP-binding protein-related contains si...   112   2e-25
At3g23860.1 68416.m02999 GTP-binding protein-related low similar...    42   3e-04
At1g80770.1 68414.m09476 expressed protein                             29   1.4  
At2g33580.1 68415.m04115 protein kinase family protein / peptido...    28   3.2  
At3g09660.1 68416.m01145 minichromosome maintenance family prote...    27   5.5  

>At1g50920.1 68414.m05725 GTP-binding protein-related similar to
           GTP-binding protein SP:Q99ME9 from [Mus musculus]
          Length = 671

 Score =  127 bits (306), Expect = 5e-30
 Identities = 56/84 (66%), Positives = 70/84 (83%)
 Frame = +1

Query: 256 FPKLDDVHPFYADLMYVLYAKDHYKLRLGQLNTARHLIDNVAKDYVRLLKYRDSLYRCEQ 435
           FP+L+ +HPFY DL++VLY KDHYKL LGQ+NTAR+LI  ++KDYV+LLKY DSLYRC+ 
Sbjct: 69  FPRLEQIHPFYGDLLHVLYNKDHYKLALGQVNTARNLISKISKDYVKLLKYGDSLYRCKC 128

Query: 436 LRRAALGRMATILKRPRANLTYLE 507
           L+ AALGRM T+LKR   +L YLE
Sbjct: 129 LKVAALGRMCTVLKRITPSLAYLE 152



 Score =  101 bits (241), Expect = 4e-22
 Identities = 45/68 (66%), Positives = 56/68 (82%)
 Frame = +2

Query: 53  MSLYNFKKIAVVPTATDFIDIILSKTQRKTPTVVHKHYKISRIRGFYIRKVQYTQQNFHD 232
           M  YNFK+I VVP   +F+DIILS+TQR+TPTVVHK YKI+R+R FY+RKV+YTQ NFH 
Sbjct: 1   MVQYNFKRITVVPNGKEFVDIILSRTQRQTPTVVHKGYKINRLRQFYMRKVKYTQTNFHA 60

Query: 233 RLSRIIQD 256
           +LS II +
Sbjct: 61  KLSAIIDE 68


>At1g10300.1 68414.m01160 GTP-binding protein-related contains
           similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4
           from [Homo sapiens];
          Length = 687

 Score =  112 bits (269), Expect = 2e-25
 Identities = 50/84 (59%), Positives = 66/84 (78%)
 Frame = +1

Query: 256 FPKLDDVHPFYADLMYVLYAKDHYKLRLGQLNTARHLIDNVAKDYVRLLKYRDSLYRCEQ 435
           FP+L ++ PFY DL++VLY KDHYKL LGQ+NTA++ I  +A DYV+LLK+ DSLYRC+ 
Sbjct: 98  FPRLKEIQPFYEDLLHVLYNKDHYKLALGQVNTAKNKISKIAMDYVKLLKHGDSLYRCKC 157

Query: 436 LRRAALGRMATILKRPRANLTYLE 507
           L+ AALGRM T++K    +L YLE
Sbjct: 158 LKVAALGRMCTVMKGIGPSLAYLE 181



 Score = 91.1 bits (216), Expect = 4e-19
 Identities = 39/68 (57%), Positives = 54/68 (79%)
 Frame = +2

Query: 53  MSLYNFKKIAVVPTATDFIDIILSKTQRKTPTVVHKHYKISRIRGFYIRKVQYTQQNFHD 232
           M  YNFKKI VVP    F+DI+LS+TQR+TPTVVHK  +I ++R FY+RKV++T+ NF++
Sbjct: 30  MVKYNFKKITVVPNGKQFVDIVLSRTQRQTPTVVHKGDRICKLRSFYMRKVKFTESNFNE 89

Query: 233 RLSRIIQD 256
           +LS II +
Sbjct: 90  KLSAIIDE 97


>At3g23860.1 68416.m02999 GTP-binding protein-related low similarity
           to SP|Q99ME9 Nucleolar GTP-binding protein 1 (Chronic
           renal failure gene protein) {Mus musculus}
          Length = 230

 Score = 41.5 bits (93), Expect = 3e-04
 Identities = 28/85 (32%), Positives = 43/85 (50%), Gaps = 5/85 (5%)
 Frame = +1

Query: 256 FPKLDDVHPFYADLMYVLYAKDHYKLRLGQLNTARHLIDNVAKDYVRLLKYR---DSLYR 426
           FP +  + P YA L++  Y   HY   + Q++  + L++ ++ +YV LL+     DS  +
Sbjct: 73  FPCVRHLDPVYASLLHQRYNMYHYDRAVRQVSVTQTLVNVMSFNYVDLLRKDDDCDSRDK 132

Query: 427 CEQLRRAALGRMATILKR--PRANL 495
           C  L   AL RM T  K   P  NL
Sbjct: 133 CRSLGVTALARMLTFAKSCIPALNL 157


>At1g80770.1 68414.m09476 expressed protein
          Length = 451

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 15/60 (25%), Positives = 28/60 (46%)
 Frame = +1

Query: 220 ELPRPTVQNYSGFPKLDDVHPFYADLMYVLYAKDHYKLRLGQLNTARHLIDNVAKDYVRL 399
           EL  P       FP+   +HP+   L+ +      Y+  LG+++  R  + +V K++  L
Sbjct: 142 ELALPLKGYMESFPRKKLLHPYERSLIDLTLGDGKYEEVLGKVDVLRKKVQSVGKEHASL 201


>At2g33580.1 68415.m04115 protein kinase family protein /
           peptidoglycan-binding LysM domain-containing protein
           protein kinase [Arabidopsis thaliana] GI:2852449;
           contains Pfam profiles PF01476: LysM domain, PF00069:
           Protein kinase domain
          Length = 664

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
 Frame = +1

Query: 34  STKLKQNEFVQL*KNC-CSPYSYGLYRHNFIENTTEN 141
           +T++   E V +  NC CS  S G Y+HN   N + N
Sbjct: 97  TTRIPTRELVVIPANCSCSSSSGGFYQHNATYNLSGN 133


>At3g09660.1 68416.m01145 minichromosome maintenance family protein
           / MCM family protein similar to SP|P49717 DNA
           replication licensing factor MCM4 (CDC21 homolog) {Mus
           musculus}; contains Pfam profile PF00493: MCM2/3/5
           family
          Length = 777

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +1

Query: 370 DNVAKDYVRLLKYRDSLYRCEQLRRAALGRMATI 471
           +NV K  VRL  Y +S    + LR A +G++ T+
Sbjct: 126 ENVMKINVRLHNYPESSISLKNLRAAYIGKLVTV 159


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,446,373
Number of Sequences: 28952
Number of extensions: 205745
Number of successful extensions: 434
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 434
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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