Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00620X
(553 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 142 1e-34
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 142 1e-34
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 142 1e-34
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.51
At1g29760.1 68414.m03639 expressed protein 31 0.51
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.1
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 6.3
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 6.3
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 6.3
At4g38440.1 68417.m05432 expressed protein 27 8.3
At2g02090.1 68415.m00145 SNF2 domain-containing protein / helica... 27 8.3
At1g34040.1 68414.m04220 alliinase family protein contains Pfam ... 27 8.3
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 142 bits (345), Expect = 1e-34
Identities = 64/84 (76%), Positives = 73/84 (86%)
Frame = +3
Query: 6 KKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIV 185
KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+
Sbjct: 6 KKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISIL 65
Query: 186 KQRLIKVDGKVRTDPTYAAGFMDL 257
QR I+VDGKVRTD TY AGFMD+
Sbjct: 66 MQRHIQVDGKVRTDKTYPAGFMDV 89
Score = 125 bits (302), Expect = 2e-29
Identities = 56/92 (60%), Positives = 70/92 (76%)
Frame = +2
Query: 257 VSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHEGRTIRY 436
VSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T++GRTIRY
Sbjct: 90 VSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRY 149
Query: 437 PDPLIKVNDSIQLDIATTKIMDFINLSLGTYV 532
PDPLIK ND+I+LD+ KI++FI +G V
Sbjct: 150 PDPLIKPNDTIKLDLEANKIVEFIKFDVGNVV 181
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 142 bits (345), Expect = 1e-34
Identities = 64/84 (76%), Positives = 73/84 (86%)
Frame = +3
Query: 6 KKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIV 185
KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+
Sbjct: 6 KKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISIL 65
Query: 186 KQRLIKVDGKVRTDPTYAAGFMDL 257
QR I+VDGKVRTD TY AGFMD+
Sbjct: 66 MQRHIQVDGKVRTDKTYPAGFMDV 89
Score = 125 bits (301), Expect = 2e-29
Identities = 56/92 (60%), Positives = 70/92 (76%)
Frame = +2
Query: 257 VSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHEGRTIRY 436
VSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T++GRTIRY
Sbjct: 90 VSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRY 149
Query: 437 PDPLIKVNDSIQLDIATTKIMDFINLSLGTYV 532
PDPLIK ND+I+LD+ KI++FI +G V
Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 142 bits (345), Expect = 1e-34
Identities = 64/84 (76%), Positives = 73/84 (86%)
Frame = +3
Query: 6 KKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIV 185
KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+
Sbjct: 6 KKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISIL 65
Query: 186 KQRLIKVDGKVRTDPTYAAGFMDL 257
QR I+VDGKVRTD TY AGFMD+
Sbjct: 66 MQRHIQVDGKVRTDKTYPAGFMDV 89
Score = 125 bits (301), Expect = 2e-29
Identities = 56/92 (60%), Positives = 70/92 (76%)
Frame = +2
Query: 257 VSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHEGRTIRY 436
VSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T++GRTIRY
Sbjct: 90 VSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRY 149
Query: 437 PDPLIKVNDSIQLDIATTKIMDFINLSLGTYV 532
PDPLIK ND+I+LD+ KI++FI +G V
Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.51
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 115 KHSRNLWGPVDGLGAYTPPSLSNIHAL 35
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.51
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 226 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 134
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.1
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 205 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 89
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 6.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 138 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 236
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 6.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 138 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 236
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 6.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 138 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 236
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 18 KRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECL 116
+R+ P W L + V++ + STGP + E L
Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELL 1172
>At2g02090.1 68415.m00145 SNF2 domain-containing protein / helicase
domain-containing protein similar to transcriptional
activator SRCAP [Homo sapiens] GI:5106572; contains Pfam
profiles PF00271: Helicase conserved C-terminal domain,
PF00176: SNF2 family N-terminal domain
Length = 763
Score = 27.1 bits (57), Expect = 8.3
Identities = 17/38 (44%), Positives = 19/38 (50%), Gaps = 2/38 (5%)
Frame = -2
Query: 135 LGKSPGASTRATCGDRLTVSVHTHRQVCPTSML--WER 28
LGK+ A T T RL H VCP S+L WER
Sbjct: 243 LGKTIQAITYLTLLSRLNNDPGPHLVVCPASVLENWER 280
>At1g34040.1 68414.m04220 alliinase family protein contains Pfam
profiles: PF04864 allinase C-terminal domain, PF04863
alliinase EGF-like domain
Length = 457
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +2
Query: 248 YGFVSIEKTNELFRLIYDVKGRFTIHRITPEEAKY 352
+G+ +++K E+ I+ + RF++ I PE Y
Sbjct: 343 FGYGTLKKRWEILNKIFSMSTRFSLETIKPEYCNY 377
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,084,889
Number of Sequences: 28952
Number of extensions: 279523
Number of successful extensions: 771
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 771
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1043173136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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