Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00615X
(524 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 131 2e-31
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 92 2e-19
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 91 3e-19
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 91 3e-19
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 72 3e-13
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 4e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 4e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 43 1e-04
At5g64220.1 68418.m08067 calmodulin-binding protein similar to a... 31 0.63
At5g57260.1 68418.m07152 cytochrome P450 71B10 identical to cyto... 28 3.3
At5g17300.1 68418.m02026 myb family transcription factor similar... 28 3.3
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 3.3
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 4.4
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 28 4.4
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 28 4.4
At3g19380.1 68416.m02458 U-box domain-containing protein contain... 28 4.4
At1g80870.1 68414.m09489 protein kinase family protein contains ... 28 4.4
At5g41450.1 68418.m05034 zinc finger (C3HC4-type RING finger) fa... 27 7.7
At2g44700.1 68415.m05563 kelch repeat-containing F-box family pr... 27 7.7
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 131 bits (317), Expect = 2e-31
Identities = 65/86 (75%), Positives = 71/86 (82%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKSDPV 177
PKLVEGLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KSDPV
Sbjct: 502 PKLVEGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPV 561
Query: 178 VSYRETVAEESDQLCLSKSPNKHNRL 255
VS+RETV + S + +SKSPNKHNRL
Sbjct: 562 VSFRETVCDRSTRTVMSKSPNKHNRL 587
Score = 124 bits (299), Expect = 4e-29
Identities = 54/87 (62%), Positives = 71/87 (81%)
Frame = +3
Query: 255 FMKAQPMPDGLPKDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNIL 434
+M+A+PM +GL + ID+GR+ PRDD K R++ L E++ +D A+KIW FGPE TGPN++
Sbjct: 588 YMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPETTGPNMV 647
Query: 435 VDCSKGVQYLNEIKDSVVAGFQWAAKK 515
VD KGVQYLNEIKDSVVAGFQWA+K+
Sbjct: 648 VDMCKGVQYLNEIKDSVVAGFQWASKE 674
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 92.3 bits (219), Expect = 2e-19
Identities = 41/86 (47%), Positives = 62/86 (72%)
Frame = +1
Query: 1 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 180
PK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV
Sbjct: 601 PKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVV 660
Query: 181 SYRETVAEESDQLCLSKSPNKHNRLS 258
S+ ETV E S C +++PNK N+L+
Sbjct: 661 SFCETVVESSSMKCFAETPNKKNKLT 686
Score = 64.9 bits (151), Expect = 3e-11
Identities = 33/90 (36%), Positives = 52/90 (57%), Gaps = 4/90 (4%)
Frame = +3
Query: 258 MKAQPMPDGLPKDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNILV 437
M A+P+ GL +DI+ G V+ + + KY++D+ AR IW FGP+ G NIL+
Sbjct: 687 MIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGTNILL 746
Query: 438 DCSKGVQ----YLNEIKDSVVAGFQWAAKK 515
D + + + +KDS+V GFQW A++
Sbjct: 747 DDTLPTEVDRNLMMGVKDSIVQGFQWGARE 776
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 91.5 bits (217), Expect = 3e-19
Identities = 40/86 (46%), Positives = 62/86 (72%)
Frame = +1
Query: 1 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 180
PK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV
Sbjct: 615 PKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVV 674
Query: 181 SYRETVAEESDQLCLSKSPNKHNRLS 258
S+ ETV E S C +++PNK N+++
Sbjct: 675 SFCETVVESSSMKCFAETPNKKNKIT 700
Score = 71.3 bits (167), Expect = 4e-13
Identities = 35/90 (38%), Positives = 54/90 (60%), Gaps = 4/90 (4%)
Frame = +3
Query: 258 MKAQPMPDGLPKDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNILV 437
M A+P+ GL +DI+ G V+ + K + KY++D+ AR IW FGP+ GPNIL+
Sbjct: 701 MIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILL 760
Query: 438 DCSKGVQ----YLNEIKDSVVAGFQWAAKK 515
D + + + +KDS+V GFQW A++
Sbjct: 761 DDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 91.5 bits (217), Expect = 3e-19
Identities = 40/86 (46%), Positives = 62/86 (72%)
Frame = +1
Query: 1 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 180
PK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV
Sbjct: 615 PKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVV 674
Query: 181 SYRETVAEESDQLCLSKSPNKHNRLS 258
S+ ETV E S C +++PNK N+++
Sbjct: 675 SFCETVVESSSMKCFAETPNKKNKIT 700
Score = 71.3 bits (167), Expect = 4e-13
Identities = 35/90 (38%), Positives = 54/90 (60%), Gaps = 4/90 (4%)
Frame = +3
Query: 258 MKAQPMPDGLPKDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNILV 437
M A+P+ GL +DI+ G V+ + K + KY++D+ AR IW FGP+ GPNIL+
Sbjct: 701 MIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILL 760
Query: 438 DCSKGVQ----YLNEIKDSVVAGFQWAAKK 515
D + + + +KDS+V GFQW A++
Sbjct: 761 DDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 71.7 bits (168), Expect = 3e-13
Identities = 32/71 (45%), Positives = 49/71 (69%)
Frame = +1
Query: 7 LVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSY 186
L++GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++ S P+VSY
Sbjct: 554 LMKGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEVSPPLVSY 613
Query: 187 RETVAEESDQL 219
RET+ + L
Sbjct: 614 RETIEGDGSNL 624
Score = 29.9 bits (64), Expect = 1.1
Identities = 21/72 (29%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = +3
Query: 222 SLKVAQQAQPSFMKAQPMPDGLPKDIDEGRVNPRDDF-KTRARYLTEKYEYDVTEARKIW 398
S+K+ + +PS + + L K + E V+ + K R + TE + ++IW
Sbjct: 683 SVKILESQKPSLGENVDPIEELKKQLVEAGVSSSSETEKDREKCKTEWSKL----LKRIW 738
Query: 399 CFGPEGTGPNIL 434
GP GPNIL
Sbjct: 739 ALGPREKGPNIL 750
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 44.8 bits (101), Expect = 4e-05
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +1
Query: 13 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 189
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 190 ETVAEESD 213
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 44.8 bits (101), Expect = 4e-05
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +1
Query: 13 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 189
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 190 ETVAEESD 213
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 43.2 bits (97), Expect = 1e-04
Identities = 23/68 (33%), Positives = 39/68 (57%), Gaps = 1/68 (1%)
Frame = +1
Query: 4 KLVEGLKRLAKSDPMVQCI-NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 180
K+ GL +LA+ DP +EE + ++ G GELHLEI + L+ + + P V
Sbjct: 509 KMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVGAPQV 567
Query: 181 SYRETVAE 204
+YRE++++
Sbjct: 568 NYRESISK 575
>At5g64220.1 68418.m08067 calmodulin-binding protein similar to
anther ethylene-upregulated calmodulin-binding protein
ER1 GI:11612392 from[Nicotiana tabacum]
Length = 1050
Score = 30.7 bits (66), Expect = 0.63
Identities = 25/84 (29%), Positives = 38/84 (45%)
Frame = +3
Query: 261 KAQPMPDGLPKDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNILVD 440
K P+P + KD+ E + + R + E++E D I EG GPNIL +
Sbjct: 610 KEPPLPGTIEKDLTE--------LEAKERLIREEFE-DKLYLWLIHKVTEEGKGPNILDE 660
Query: 441 CSKGVQYLNEIKDSVVAGFQWAAK 512
+GV +L + G+ WA K
Sbjct: 661 DGQGVLHL-----AAALGYDWAIK 679
>At5g57260.1 68418.m07152 cytochrome P450 71B10 identical to
cytochrome P450 71B10 (SP:Q9LVD2) [Arabidopsis thaliana]
Length = 1483
Score = 28.3 bits (60), Expect = 3.3
Identities = 22/58 (37%), Positives = 32/58 (55%)
Frame = -1
Query: 368 FVLFSKIPSASLEVIAGIYATLINVFWQTIRHGLSLHERRLCLLGDFERQSWSDSSAT 195
FVLF++I S +EV +Y TLI+ R G +L+ R +LGD E++ S T
Sbjct: 844 FVLFNRILSVGIEVDEFLYVTLID---GICRKG-NLN-RAFSMLGDMEQRGIQPSILT 896
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 28.3 bits (60), Expect = 3.3
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -1
Query: 209 DSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 84
D+ + S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +1
Query: 58 INEESGEHIVAGAGELHLEICLKDLEEDH 144
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 91 GAGELHLEICLKDLEEDHACIPIKK 165
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 27.9 bits (59), Expect = 4.4
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +1
Query: 322 AMTSRLALGILLKSTNTMLPKPVRFGALAPKVPAPTSWSIAPKE 453
A+ SR AL + +PKPV+ P+ PAP S S +E
Sbjct: 835 AIASRSALFEKIPPQEPSIPKPVKASPKTPESPAPESNSKEQEE 878
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -3
Query: 195 GLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 79
G TV+ DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At3g19380.1 68416.m02458 U-box domain-containing protein contains
similarity to immediate-early fungal elicitor protein
CMPG1 GI:14582200 [Petroselinum crispum]; contains Pfam
profile PF04564: U-box domain
Length = 421
Score = 27.9 bits (59), Expect = 4.4
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -2
Query: 415 PSGPKHQILRASVTSYSYFSVRYRARVLKSSRGFTRPS 302
P+ + + +AS + ++ SVR RA L+ RGF R S
Sbjct: 102 PTSVRALLSQASAITGTHVSVRSRAAALRRLRGFARDS 139
>At1g80870.1 68414.m09489 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 692
Score = 27.9 bits (59), Expect = 4.4
Identities = 17/64 (26%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = -1
Query: 266 SLHERRLCLLGDFE-RQSWSDSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAP 90
S+H+R G+F R+ W S + S+ ++ +G + WS R++S +S
Sbjct: 492 SVHDREGEAAGEFSFRRGWRRKSNSSSKKKKKNNNGSMGSEMWSGDLFSRELSSTTS-MR 550
Query: 89 ATMC 78
T+C
Sbjct: 551 GTLC 554
>At5g41450.1 68418.m05034 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 164
Score = 27.1 bits (57), Expect = 7.7
Identities = 15/52 (28%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = +3
Query: 243 AQPSFMKAQPMPDGLPKDIDEGRVN-PRDDFKTRARYLTEKYEYDVTEARKI 395
A+ + + P PD L +DI+ G V P+ Y+T +E + E + I
Sbjct: 50 ARETHQNSHPPPDQLQQDIETGHVTLPQPQQNIAVGYMTWIHETTILEFKDI 101
>At2g44700.1 68415.m05563 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif;
Length = 368
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = +3
Query: 294 DIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 401
D+D R ++ + ++ EK +V+E+++IWC
Sbjct: 294 DLDNARSVGIGEYYGKLAFIWEKPSLNVSESKEIWC 329
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,412,741
Number of Sequences: 28952
Number of extensions: 279342
Number of successful extensions: 917
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 878
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 912
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 967280384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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