Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00604
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 148 6e-37
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 78 1e-15
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 59 5e-10
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 48 1e-06
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 47 2e-06
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 44 2e-05
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 39 5e-04
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 36 0.006
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 28 0.97
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 28 0.97
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 28 1.3
SPBC418.02 |||NatA N-acetyltransferase complex subunit |Schizosa... 28 1.3
SPCC663.05c |cia1||histone chaperone Cia1|Schizosaccharomyces po... 27 2.3
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 3.0
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 26 3.9
SPBC530.06c |||translation initiation factor eIF3 alpha subunit ... 26 5.2
SPAC4A8.09c |cwf21||complexed with Cdc5 protein Cwf21 |Schizosac... 25 6.9
SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 6.9
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 25 9.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 148 bits (359), Expect = 6e-37
Identities = 68/84 (80%), Positives = 76/84 (90%)
Frame = +2
Query: 2 KDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTD 181
+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDADGNGTIDF EFLTMMARKMKDTD
Sbjct: 23 RDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTD 82
Query: 182 SEEEIREAFRVFDKDGNGFISAAE 253
+EEE+REAF+VFDKDGNG+I+ E
Sbjct: 83 NEEEVREAFKVFDKDGNGYITVEE 106
Score = 63.3 bits (147), Expect = 3e-11
Identities = 27/42 (64%), Positives = 36/42 (85%)
Frame = +1
Query: 259 HVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTSK 384
HV+T+LGE+L+ EEV +MIREAD DGDG +NYEEF +++SK
Sbjct: 109 HVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISSK 150
Score = 56.0 bits (129), Expect = 4e-09
Identities = 25/55 (45%), Positives = 38/55 (69%)
Frame = +2
Query: 2 KDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARK 166
KDG+G IT +EL V+ SLG+ ++ E+ DMI E D DG+G I++ EF +++ K
Sbjct: 96 KDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISSK 150
Score = 49.6 bits (113), Expect = 4e-07
Identities = 30/84 (35%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Frame = +2
Query: 5 DGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTD 181
DG+GTI E T+M R + E E+++ D DGNG I E LT + + +
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEE-LTHVLTSLGERL 118
Query: 182 SEEEIREAFRVFDKDGNGFISAAE 253
S+EE+ + R D DG+G I+ E
Sbjct: 119 SQEEVADMIREADTDGDGVINYEE 142
Score = 45.2 bits (102), Expect = 8e-06
Identities = 19/41 (46%), Positives = 28/41 (68%)
Frame = +1
Query: 262 VMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTSK 384
VM +LG+ T E+ +MI E D DG+G +++ EF+TMM K
Sbjct: 37 VMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARK 77
Score = 30.3 bits (65), Expect = 0.24
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Frame = +1
Query: 265 MTNLGEKLTDEEVDEMIREA----DIDGDGQVNYEEFVTMMTS 381
+T + K+ D + +E +REA D DG+G + EE ++TS
Sbjct: 71 LTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113
Score = 27.9 bits (59), Expect = 1.3
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +2
Query: 152 MMARKMKDTDSEEEIREAFRVFDKDGNGFISAAE 253
M R + D + E REAF +FD+D +G I++ E
Sbjct: 1 MTTRNLTD-EQIAEFREAFSLFDRDQDGNITSNE 33
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 77.8 bits (183), Expect = 1e-15
Identities = 39/85 (45%), Positives = 58/85 (68%)
Frame = +2
Query: 5 DGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDS 184
D DG I T +G+V+RSLG N T+AEL + NE+ ID +F++ ++ K+++T+S
Sbjct: 21 DKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEKKFMSFVSNKLRETES 76
Query: 185 EEEIREAFRVFDKDGNGFISAAERA 259
EEE +AFRVFDKD +G+I A+ A
Sbjct: 77 EEEYIKAFRVFDKDNSGYIETAKFA 101
Score = 39.5 bits (88), Expect = 4e-04
Identities = 18/40 (45%), Positives = 25/40 (62%)
Frame = +1
Query: 265 MTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTSK 384
M LGEKL+D EV M++EAD G +Y +FV + +K
Sbjct: 104 MKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQRIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 59.3 bits (137), Expect = 5e-10
Identities = 29/80 (36%), Positives = 44/80 (55%)
Frame = +2
Query: 5 DGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDS 184
D D I EL MR+LG N ++E+ ++ + D G G + +F+ +M K+ + D
Sbjct: 49 DKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDP 108
Query: 185 EEEIREAFRVFDKDGNGFIS 244
EEI+ AF +FD D G IS
Sbjct: 109 LEEIKRAFELFDDDETGKIS 128
Score = 44.0 bits (99), Expect = 2e-05
Identities = 17/40 (42%), Positives = 27/40 (67%)
Frame = +1
Query: 256 RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 375
R V L E + D+E++ MI E D+D DG++N +EF+ +M
Sbjct: 133 RRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIM 172
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 47.6 bits (108), Expect = 1e-06
Identities = 26/80 (32%), Positives = 45/80 (56%)
Frame = +2
Query: 14 GTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEE 193
G+I E ++ S+ NP + L + VD DG G +DF EF+ ++ + EE+
Sbjct: 39 GSIDRNEFLSIP-SVASNPLASRL---FSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEK 94
Query: 194 IREAFRVFDKDGNGFISAAE 253
++ AF+++D D +G+IS E
Sbjct: 95 LKFAFKIYDIDRDGYISNGE 114
Score = 34.3 bits (75), Expect = 0.015
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +1
Query: 262 VMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEF 363
V TNL E + VD+ I E D D DG++++EEF
Sbjct: 123 VGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEF 156
Score = 25.4 bits (53), Expect = 6.9
Identities = 16/51 (31%), Positives = 25/51 (49%)
Frame = +2
Query: 101 EVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAE 253
++DA+ +G+ID EFL+ + S F V D+DG G + E
Sbjct: 32 KIDANQSGSIDRNEFLS-----IPSVASNPLASRLFSVVDEDGGGDVDFQE 77
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 47.2 bits (107), Expect = 2e-06
Identities = 20/40 (50%), Positives = 32/40 (80%)
Frame = +1
Query: 256 RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 375
R+V+T+LGEKL++EE+DE+++ + DG VNY +FV M+
Sbjct: 100 RYVLTSLGEKLSNEEMDELLKGVPVK-DGMVNYHDFVQMI 138
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 44.0 bits (99), Expect = 2e-05
Identities = 27/80 (33%), Positives = 40/80 (50%)
Frame = +2
Query: 2 KDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTD 181
KDGDG I +++ T++ SL Q+ +E D IN + N I+ FLT M +
Sbjct: 59 KDGDGNIGREDVKTMLTSLNQDASE----DSINHMFESINPPINLAAFLTAMGSMLCRIS 114
Query: 182 SEEEIREAFRVFDKDGNGFI 241
++ EAF FD +G I
Sbjct: 115 PRNDLLEAFSTFDDTQSGKI 134
Score = 27.9 bits (59), Expect = 1.3
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 295 EEVDEMIREADIDGDGQVNYEEFVTMMTS 381
+E+ E D DGDG + E+ TM+TS
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTS 76
Score = 27.5 bits (58), Expect = 1.7
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +2
Query: 188 EEIREAFRVFDKDGNGFISAAERAT 262
+E++EAF + DKDG+G I + T
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKT 72
Score = 25.0 bits (52), Expect = 9.1
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 80 ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAF 208
EL++ +D DG+G I + TM+ +D SE+ I F
Sbjct: 49 ELKEAFALLDKDGDGNIGREDVKTMLTSLNQDA-SEDSINHMF 90
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 39.1 bits (87), Expect = 5e-04
Identities = 20/54 (37%), Positives = 32/54 (59%)
Frame = +2
Query: 92 MINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAE 253
+ N DAD NG IDF EF+ ++ + + +++ AF+++D D NG IS E
Sbjct: 68 VFNVFDADKNGYIDFKEFICALSVTSRG-ELNDKLIWAFQLYDLDNNGLISYDE 120
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 35.5 bits (78), Expect = 0.006
Identities = 19/51 (37%), Positives = 28/51 (54%)
Frame = +2
Query: 83 LQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNG 235
L D N++D DG G +D P T+ A + S +E+REA R + D +G
Sbjct: 21 LTDQFNKLDVDGKGYLDQP--TTIKAFEDSKKGSYDEVREAIREVNVDSSG 69
Score = 29.1 bits (62), Expect = 0.56
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +1
Query: 295 EEVDEMIREADIDGDGQVNYEEFV 366
+EV E IRE ++D G+V E+FV
Sbjct: 54 DEVREAIREVNVDSSGRVEPEDFV 77
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 28.3 bits (60), Expect = 0.97
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +1
Query: 283 KLTDEEVDEMIREADIDGDGQVNYEEFVTMM 375
KL+ ++++++ ADID DG +++EF M
Sbjct: 36 KLSSDKLEKIWDLADIDDDGMFDFDEFAIAM 66
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 28.3 bits (60), Expect = 0.97
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +2
Query: 95 INEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDK 223
I+E D + NG + F + + +KD+ EE+ ++F + D+
Sbjct: 545 ISESDDESNGDMIRDSFDRLSSESIKDSQKTEELHDSFGINDE 587
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/53 (20%), Positives = 20/53 (37%)
Frame = +1
Query: 13 WHHHDQRAGHRDEVARTEPHRSRTSRHDQ*SRRGRKRHDRLSRVLDNDGAQDE 171
W H + + H D+ R PH H H + R + ++G + +
Sbjct: 252 WKHEEHCSCHHDKFPRPVPHNGTKPDHKPWKHEEHCHHGKFPRPIPHNGTKPD 304
Score = 25.0 bits (52), Expect = 9.1
Identities = 12/55 (21%), Positives = 22/55 (40%), Gaps = 2/55 (3%)
Frame = +1
Query: 13 WHHHDQRAGHRDEVARTEPHRSRTSRHD--Q*SRRGRKRHDRLSRVLDNDGAQDE 171
W H + + H D+ R PH H + HD+ R + ++G + +
Sbjct: 223 WKHEEHCSCHHDKFPRPVPHNGTKPDHKPWKHEEHCSCHHDKFPRPVPHNGTKPD 277
>SPBC418.02 |||NatA N-acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = -1
Query: 471 SNTMLILCVMKQNVYLNSLLFTHTLAGGSLRRHHGDE 361
S T+L + +M QN +LN+ F H A + R+ + +E
Sbjct: 199 SQTVLEILLMNQNSFLNNFNFEHIKADFAFRQKNYEE 235
>SPCC663.05c |cia1||histone chaperone Cia1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 27.1 bits (57), Expect = 2.3
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 262 VMTNLGEKLTDEEVDEMIREADIDGDGQVNYEE 360
V + E+ +EE DEM E D +G+G EE
Sbjct: 170 VQPDADEEEEEEEADEMEEEFDEEGEGDEEEEE 202
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 3.0
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -2
Query: 89 LEVRLLWGSVLATSSRCPALWS*WCHR 9
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 26.2 bits (55), Expect = 3.9
Identities = 16/70 (22%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = +2
Query: 17 TITTKELGTVMRSLGQ-NPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEE 193
T + GT+ S+G + E + ++++ T DFPE L+ +++ EE
Sbjct: 545 TTSLHPFGTLYESIGTIGDPKVEYKAILHDFSCH---TYDFPESLSHCVKRLPTPIPAEE 601
Query: 194 IREAFRVFDK 223
+++ + + DK
Sbjct: 602 LQKRYNLRDK 611
>SPBC530.06c |||translation initiation factor eIF3 alpha subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 25.8 bits (54), Expect = 5.2
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 351 IDLAVAVDIGLANHLVDLLVSEFLSEVGHDVARSAAEMKPLP 226
+D++V +D+ +H L S LS HDV + E+ P
Sbjct: 28 VDVSVDIDVIFPDHTQTLSFSLLLSNTIHDVRQVILELMLAP 69
>SPAC4A8.09c |cwf21||complexed with Cdc5 protein Cwf21
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 293
Score = 25.4 bits (53), Expect = 6.9
Identities = 14/44 (31%), Positives = 19/44 (43%)
Frame = +1
Query: 10 RWHHHDQRAGHRDEVARTEPHRSRTSRHDQ*SRRGRKRHDRLSR 141
R H+D + + DE+ R SR + RR RHD R
Sbjct: 215 RRRHYDSYSSY-DELERRRSSNESYSRRSELPRRDYNRHDERER 257
>SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 700
Score = 25.4 bits (53), Expect = 6.9
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -1
Query: 429 YLNSLLFTHTLAGGSLRRHHGDELFVIDLAVAV 331
YLNSLL H G L++ H + LAVA+
Sbjct: 299 YLNSLLSVHEKDGVLLQKFHNSYVQYQKLAVAL 331
>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
Usp104|Schizosaccharomyces pombe|chr 1|||Manual
Length = 695
Score = 25.0 bits (52), Expect = 9.1
Identities = 9/38 (23%), Positives = 19/38 (50%)
Frame = +2
Query: 89 DMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIRE 202
D + + + T + PE + ++ K+KD + E + E
Sbjct: 479 DCLEVLQISVDDTSNIPEIIARLSEKLKDREESEAVTE 516
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,871,732
Number of Sequences: 5004
Number of extensions: 30195
Number of successful extensions: 141
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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