BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00603X (449 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g67520.1 68414.m07692 lectin protein kinase family protein co... 27 4.4 At3g55940.1 68416.m06216 phosphoinositide-specific phospholipase... 27 7.7 At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy... 27 7.7 At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy... 27 7.7 >At1g67520.1 68414.m07692 lectin protein kinase family protein contains Pfam domains PF00069: Protein kinase domain and PF01453: Lectin (probable mannose binding) Length = 587 Score = 27.5 bits (58), Expect = 4.4 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +3 Query: 135 NNENIYSGFWYFNFTVNT 188 N+EN+Y G W+ N +NT Sbjct: 55 NSENLYLGIWFNNLYLNT 72 >At3g55940.1 68416.m06216 phosphoinositide-specific phospholipase C, putative similar to phosphoinositide specific phospholipase C GI:857374 from [Arabidopsis thaliana] Length = 584 Score = 26.6 bits (56), Expect = 7.7 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +2 Query: 188 DVPYAVYIVHMLHVKDFIGGQLSSECQQM 274 D P + Y ++ H G QLSS+C ++ Sbjct: 106 DAPLSHYFIYTGHNSYLTGNQLSSDCSEL 134 >At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehydrogenase family protein weak similarity to SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 462 Score = 26.6 bits (56), Expect = 7.7 Identities = 14/65 (21%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Frame = +3 Query: 27 QHTF-TLEIENNCIFNLMNTCTYERYIKLQL*LETHYNNENIYSGFWYFNFTVNTMYPMQ 203 +H F + + + L+ C Y +Y++ +L T Y +E + GF N + P++ Sbjct: 89 EHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILKDGF-CLNMVDTPLCPLK 147 Query: 204 YISSI 218 ++++ Sbjct: 148 VVTNL 152 >At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehydrogenase family protein weak similarity to SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 462 Score = 26.6 bits (56), Expect = 7.7 Identities = 14/65 (21%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Frame = +3 Query: 27 QHTF-TLEIENNCIFNLMNTCTYERYIKLQL*LETHYNNENIYSGFWYFNFTVNTMYPMQ 203 +H F + + + L+ C Y +Y++ +L T Y +E + GF N + P++ Sbjct: 89 EHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILKDGF-CLNMVDTPLCPLK 147 Query: 204 YISSI 218 ++++ Sbjct: 148 VVTNL 152 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,240,855 Number of Sequences: 28952 Number of extensions: 207463 Number of successful extensions: 428 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 425 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 428 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 732537840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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