Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00588X
(595 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48540.2 68414.m05428 leucine-rich repeat family protein 34 0.062
At1g48540.1 68414.m05427 leucine-rich repeat family protein 34 0.062
At1g78610.1 68414.m09161 mechanosensitive ion channel domain-con... 33 0.11
At3g15690.2 68416.m01989 biotin carboxyl carrier protein of acet... 31 0.77
At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-contain... 29 2.3
At4g18370.1 68417.m02724 protease HhoA, chloroplast (SPPA) (HHOA... 28 4.1
At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse... 28 5.4
At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-th... 28 5.4
At1g67060.1 68414.m07626 expressed protein 28 5.4
At1g54510.1 68414.m06217 protein kinase family protein contains ... 28 5.4
At1g28200.1 68414.m03461 GRAM domain-containing protein / ABA-re... 28 5.4
At4g01100.1 68417.m00148 mitochondrial substrate carrier family ... 27 7.1
At5g59920.1 68418.m07514 DC1 domain-containing protein contains ... 27 9.4
>At1g48540.2 68414.m05428 leucine-rich repeat family protein
Length = 1051
Score = 34.3 bits (75), Expect = 0.062
Identities = 22/87 (25%), Positives = 40/87 (45%)
Frame = +3
Query: 291 FSTKRVFADASSINNHLYNLVTGGDYINAVKTVRSLVDNQGSDVCRDVVSQLVSHGIKNA 470
F+ R + S ++ HL LV + + ++ + +L QG DV +++S +
Sbjct: 222 FNHLRTVSYLSQVSCHLVKLVLRNNALTTLRGIENLKSLQGLDVSYNIISNFSELEFLWS 281
Query: 471 MSFAYKLWHEGHKTSSKITSRANSNSY 551
+S +LW EG+ RA+ SY
Sbjct: 282 LSQLKELWLEGNPVCCARWYRAHVFSY 308
>At1g48540.1 68414.m05427 leucine-rich repeat family protein
Length = 1063
Score = 34.3 bits (75), Expect = 0.062
Identities = 22/87 (25%), Positives = 40/87 (45%)
Frame = +3
Query: 291 FSTKRVFADASSINNHLYNLVTGGDYINAVKTVRSLVDNQGSDVCRDVVSQLVSHGIKNA 470
F+ R + S ++ HL LV + + ++ + +L QG DV +++S +
Sbjct: 222 FNHLRTVSYLSQVSCHLVKLVLRNNALTTLRGIENLKSLQGLDVSYNIISNFSELEFLWS 281
Query: 471 MSFAYKLWHEGHKTSSKITSRANSNSY 551
+S +LW EG+ RA+ SY
Sbjct: 282 LSQLKELWLEGNPVCCARWYRAHVFSY 308
>At1g78610.1 68414.m09161 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 856
Score = 33.5 bits (73), Expect = 0.11
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = -1
Query: 577 DEFNSLLVEYELEFAREVIFDDVLWPSCHSLYANDMAFLMPCETSCETTS 428
D+ N + E+E + A IF +V P +YAND+ +P + + +T S
Sbjct: 519 DKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLS 568
>At3g15690.2 68416.m01989 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 263
Score = 30.7 bits (66), Expect = 0.77
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -1
Query: 67 DVTGSVAVVHRHDGDPVGY 11
DVTG V + R DG+PVGY
Sbjct: 227 DVTGEVVKILREDGEPVGY 245
>At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-containing
protein similar to SP|Q06881 Biotin carboxyl carrier
protein of acetyl-CoA carboxylase (BCCP) {Anabaena sp.};
contains Pfam profile PF00364: Biotin-requiring enzyme
Length = 274
Score = 29.1 bits (62), Expect = 2.3
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1
Query: 76 VVPDVTGSVAVVHRHDGDPVGY 11
V DV+G + + R DG+PVGY
Sbjct: 235 VESDVSGEIVKILREDGEPVGY 256
>At4g18370.1 68417.m02724 protease HhoA, chloroplast (SPPA) (HHOA)
identical to SP|Q9SEL7 Protease HhoA, chloroplast
precursor (EC 3.4.21.-) {Arabidopsis thaliana}
Length = 323
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = +3
Query: 3 ESKYPTGSPSCLWTTATLPVTSGTTASKLLPSARGQSLSGALQT 134
+S + G+P T T+ V SG + +PS G+S+S A+QT
Sbjct: 216 QSCFAIGNPYGYENTLTIGVVSGL--GREIPSPNGKSISEAIQT 257
>At5g27380.1 68418.m03269 glutathione synthetase (GSH2)
non-consensus AT donor splice site at exon 6, AC
acceptor splice site at exon 7; identical to
Swiss-Prot:P46416 glutathione synthetase, chloroplast
precursor (Glutathione synthase) (GSH synthetase)
(GSH-S) [Arabidopsis thaliana]
Length = 478
Score = 27.9 bits (59), Expect = 5.4
Identities = 8/26 (30%), Positives = 17/26 (65%)
Frame = -1
Query: 541 EFAREVIFDDVLWPSCHSLYANDMAF 464
EF +++++D ++W S H L D ++
Sbjct: 16 EFVQKLVYDALVWSSLHGLVVGDKSY 41
>At4g34570.1 68417.m04912 bifunctional dihydrofolate
reductase-thymidylate synthase 2 / DHFR-TS (THY-2)
identical to SP|Q05763
Length = 565
Score = 27.9 bits (59), Expect = 5.4
Identities = 9/35 (25%), Positives = 18/35 (51%)
Frame = -1
Query: 502 PSCHSLYANDMAFLMPCETSCETTSRQTSEPWLST 398
PSC +++ ++ + C+T T +PW S+
Sbjct: 191 PSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSS 225
>At1g67060.1 68414.m07626 expressed protein
Length = 234
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = -1
Query: 592 IVMFADEFNSLLVEYELEFAREVIFDDVLWPSCHSLYANDMAFLMPCETSC 440
+++F NSL Y+ I+DD++ HS A A + PC T C
Sbjct: 165 VILFIGVMNSLFSVYD-------IYDDLISRRVHSSDAEKFAEICPCCTGC 208
>At1g54510.1 68414.m06217 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 612
Score = 27.9 bits (59), Expect = 5.4
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +3
Query: 57 PVTSGTTASKLLPSARGQSL 116
PV+ G T SK++PSAR SL
Sbjct: 388 PVSGGGTTSKIIPSARRTSL 407
>At1g28200.1 68414.m03461 GRAM domain-containing protein /
ABA-responsive protein-related similar to ABA-responsive
protein [Hordeum vulgare] GI:4103635; contains Pfam
profile PF02893: GRAM domain
Length = 259
Score = 27.9 bits (59), Expect = 5.4
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 7/64 (10%)
Frame = +3
Query: 243 LWSRYE--LPADLRTQTEFSTKRV-----FADASSINNHLYNLVTGGDYINAVKTVRSLV 401
LWS Y+ LPA+ ST RV + SI+NH + + Y +AVK+++ V
Sbjct: 195 LWSYYKVVLPANQLKAVNPSTSRVNTSDKYIQVISIDNHEFWFMGFVTYESAVKSLQEAV 254
Query: 402 DNQG 413
+ G
Sbjct: 255 QSHG 258
>At4g01100.1 68417.m00148 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 352
Score = 27.5 bits (58), Expect = 7.1
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 181 YSDNSDDIQNLERELGKKGLYYGAGTNC 264
YS ++++ R G +GL+ G GTNC
Sbjct: 76 YSGTVQGLKHIWRTEGLRGLFKGNGTNC 103
>At5g59920.1 68418.m07514 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 710
Score = 27.1 bits (57), Expect = 9.4
Identities = 12/42 (28%), Positives = 25/42 (59%)
Frame = -3
Query: 515 RCLVALVPQLVRERHGVLDAMRDELRDDVATDVGALVVDETA 390
RC V+++ +++++ D D++ DDV+ D V D+T+
Sbjct: 624 RCKVSVILKILKDSEDGSDDGSDDVSDDVSDDPSNDVSDDTS 665
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,503,479
Number of Sequences: 28952
Number of extensions: 210214
Number of successful extensions: 841
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 812
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 841
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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