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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00518
         (607 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g35740.2 68417.m05073 DNA helicase (RECQl3) identical to DNA ...    31   0.45 
At4g35740.1 68417.m05072 DNA helicase (RECQl3) identical to DNA ...    31   0.45 
At5g50110.1 68418.m06205 methyltransferase-related contains weak...    30   1.0  
At5g57500.1 68418.m07185 expressed protein                             29   2.4  
At3g52260.2 68416.m05743 pseudouridine synthase family protein s...    28   5.5  
At3g52260.1 68416.m05744 pseudouridine synthase family protein s...    28   5.5  

>At4g35740.2 68417.m05073 DNA helicase (RECQl3) identical to DNA
           Helicase [Arabidopsis thaliana] GI:11121447; contains
           Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
           Helicase conserved C-terminal domain
          Length = 620

 Score = 31.5 bits (68), Expect = 0.45
 Identities = 22/81 (27%), Positives = 40/81 (49%), Gaps = 4/81 (4%)
 Frame = -3

Query: 395 LFPMR*SRAEVPRLGSKPSHAKNAPPTMLTIRAVKNPMILKSQ----*LLTDIQYKLTLD 228
           L  +R S A+VP L    + A      ++    ++NP++LKS      +  +++YK  LD
Sbjct: 89  LSTLRDSLADVPVLALTATAAPKVQKDVIDSLNLRNPLVLKSSFNRPNIFYEVRYKDLLD 148

Query: 227 NLYLFIDQQFLDNSGAQCSVL 165
           N Y  +    L + G  C+++
Sbjct: 149 NAYTDLG-NLLKSCGNICAII 168


>At4g35740.1 68417.m05072 DNA helicase (RECQl3) identical to DNA
           Helicase [Arabidopsis thaliana] GI:11121447; contains
           Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
           Helicase conserved C-terminal domain
          Length = 713

 Score = 31.5 bits (68), Expect = 0.45
 Identities = 22/81 (27%), Positives = 40/81 (49%), Gaps = 4/81 (4%)
 Frame = -3

Query: 395 LFPMR*SRAEVPRLGSKPSHAKNAPPTMLTIRAVKNPMILKSQ----*LLTDIQYKLTLD 228
           L  +R S A+VP L    + A      ++    ++NP++LKS      +  +++YK  LD
Sbjct: 182 LSTLRDSLADVPVLALTATAAPKVQKDVIDSLNLRNPLVLKSSFNRPNIFYEVRYKDLLD 241

Query: 227 NLYLFIDQQFLDNSGAQCSVL 165
           N Y  +    L + G  C+++
Sbjct: 242 NAYTDLG-NLLKSCGNICAII 261


>At5g50110.1 68418.m06205 methyltransferase-related contains weak
           similarity to Methyltransferase gidB (EC 2.1.-.-)
           (Glucose inhibited division protein B).
           (Swiss-Prot:Q93D95) [Streptococcus mutans]
          Length = 208

 Score = 30.3 bits (65), Expect = 1.0
 Identities = 22/78 (28%), Positives = 34/78 (43%)
 Frame = +1

Query: 259 RSYCDLRIMGFFTALIVNIVGGAFFAWEGFDPNRGTSARLQRIGNNGGKHCSFSPVVLRE 438
           R+  ++R++  +   +V I GG F A +G DP          +   GG     SPV    
Sbjct: 118 RAVAEMRVLAEYCLPLVRI-GGLFVAAKGHDPKEEVQNAENAVRLLGGSILQISPVDSHS 176

Query: 439 FNGRKHYIKVDCRRHDST 492
             G++    V CR+  ST
Sbjct: 177 PYGQR--TTVVCRKDHST 192


>At5g57500.1 68418.m07185 expressed protein
          Length = 318

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 13/31 (41%), Positives = 20/31 (64%)
 Frame = +2

Query: 113 KFDSQFVFVKLTKLFQEVTLSTELQSYREIV 205
           K D +FVF  LTK  Q+V ++ E+  Y +I+
Sbjct: 102 KVDVKFVFCNLTKEDQKVLVALEIMRYDDII 132


>At3g52260.2 68416.m05743 pseudouridine synthase family protein
           similar to SP|P39219 Ribosomal large subunit
           pseudouridine synthase A (EC 4.2.1.70) (Pseudouridylate
           synthase) (Uracil hydrolyase) {Escherichia coli};
           contains Pfam profile PF00849: RNA pseudouridylate
           synthase
          Length = 369

 Score = 27.9 bits (59), Expect = 5.5
 Identities = 15/36 (41%), Positives = 19/36 (52%)
 Frame = -1

Query: 526 QQLSVFTNLWSWCYHGGGSQL*YNASGH*IPVIRLG 419
           QQ +V T L  WC+    S +    S H +PV RLG
Sbjct: 124 QQRTVLTQL-QWCFGKNDSYIGSRESPHPVPVHRLG 158


>At3g52260.1 68416.m05744 pseudouridine synthase family protein
           similar to SP|P39219 Ribosomal large subunit
           pseudouridine synthase A (EC 4.2.1.70) (Pseudouridylate
           synthase) (Uracil hydrolyase) {Escherichia coli};
           contains Pfam profile PF00849: RNA pseudouridylate
           synthase
          Length = 294

 Score = 27.9 bits (59), Expect = 5.5
 Identities = 15/36 (41%), Positives = 19/36 (52%)
 Frame = -1

Query: 526 QQLSVFTNLWSWCYHGGGSQL*YNASGH*IPVIRLG 419
           QQ +V T L  WC+    S +    S H +PV RLG
Sbjct: 124 QQRTVLTQL-QWCFGKNDSYIGSRESPHPVPVHRLG 158


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,107,072
Number of Sequences: 28952
Number of extensions: 216426
Number of successful extensions: 401
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 401
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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