Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00515
(450 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 39 0.002
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 35 0.022
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 34 0.051
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 29 1.5
At3g26430.1 68416.m03294 GDSL-motif lipase/hydrolase family prot... 29 1.5
At1g74010.1 68414.m08571 strictosidine synthase family protein s... 29 1.9
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 28 2.5
At3g47780.1 68416.m05205 ABC transporter family protein transpor... 27 5.9
At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 27 5.9
At5g14770.1 68418.m01733 pentatricopeptide (PPR) repeat-containi... 27 7.7
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 38.7 bits (86), Expect = 0.002
Identities = 17/59 (28%), Positives = 29/59 (49%)
Frame = +2
Query: 68 KVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQELLNAKNYP 244
K+ P+ + L Y+LD IY+W+G+ +E+ A A+E L ++N P
Sbjct: 261 KMEPIDGDLSKSMLENTKCYLLDC-GAEIYIWVGRVTQVDERKAASQSAEEFLASENRP 318
Score = 28.3 bits (60), Expect = 2.5
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +2
Query: 59 GKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQEL 223
GK + PF + +L+ + +ILDT IY + G + E+++A+ Q L
Sbjct: 144 GKRAIRLKQVPFARSSLNHDDVFILDT-EEKIYQFNGANSNIQERAKALEVVQYL 197
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 35.1 bits (77), Expect = 0.022
Identities = 15/45 (33%), Positives = 27/45 (60%)
Frame = +2
Query: 92 FKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQELL 226
F Q++L+ ++ ++LD S +YVWIG + K +A+T + L
Sbjct: 639 FVQDDLTTEDVFLLDCQS-EVYVWIGSNSNIKSKEEALTLGLKFL 682
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 33.9 bits (74), Expect = 0.051
Identities = 19/75 (25%), Positives = 39/75 (52%)
Frame = +2
Query: 2 EFERNEASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQAT 181
+ +R+ S+ L + +K T + F Q++L ++ +ILD + ++VW+G+Q
Sbjct: 615 KIKRDGESDPHLFSCTYTNESLKATEIFN-FTQDDLMTEDIFILDCHT-EVFVWVGQQVD 672
Query: 182 ANEKSQAMTKAQELL 226
+K QA+ + L
Sbjct: 673 PKKKPQALDIGENFL 687
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 29.1 bits (62), Expect = 1.5
Identities = 17/53 (32%), Positives = 29/53 (54%)
Frame = +2
Query: 59 GKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQ 217
GK V PF + +L+ + YILDT S I+ + G ++ E+++A+ Q
Sbjct: 146 GKHVVHVKEVPFARSSLNHDDIYILDTKS-KIFQFNGSNSSIQERAKALEVVQ 197
>At3g26430.1 68416.m03294 GDSL-motif lipase/hydrolase family protein
similar to early nodulin ENOD8 [Medicago sativa]
GI:304037; contains InterPro Entry IPR001087 Lipolytic
enzyme, G-D-S-L family
Length = 380
Score = 29.1 bits (62), Expect = 1.5
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -3
Query: 214 SFRHGLRFLVCGSLLPDPNVNVARYGVQNI 125
+F HG F GS + PN +A+ GV I
Sbjct: 97 NFSHGANFATAGSTVRPPNATIAQSGVSPI 126
>At1g74010.1 68414.m08571 strictosidine synthase family protein
similar to strictosidine synthase [Rauvolfia
serpentina][SP|P15324]; contains strictosidine synthase
domain PF03088
Length = 325
Score = 28.7 bits (61), Expect = 1.9
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 5 FERNEASNVILSEVSDATGKIKVTPLSKPF 94
F +NE N +LSEV++ G + + L+ PF
Sbjct: 290 FLKNEFGNTLLSEVNEFNGHLYIGTLTGPF 319
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 28.3 bits (60), Expect = 2.5
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 59 GKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQEL 223
GK V PF + +L+ + +ILDT IY + G + E+++A+ Q L
Sbjct: 146 GKRAVHLKQVPFARSSLNHDDVFILDT-KEKIYQFNGANSNIQERAKALVVIQYL 199
Score = 27.1 bits (57), Expect = 5.9
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +1
Query: 256 VTRVLQNTEPAAFKQYFFTW 315
+TRV+Q EP +FK F +W
Sbjct: 325 ITRVIQGYEPHSFKSNFDSW 344
>At3g47780.1 68416.m05205 ABC transporter family protein transport
protein ABC-C, Homo sapiens, PIR2:S71363
Length = 935
Score = 27.1 bits (57), Expect = 5.9
Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +1
Query: 85 EAFQARELESTERVYFGHHIWQHLRLDREAGYRKREISGHDESSGIVER*ELSFV-GAVT 261
E A +L +T+R F HIW + +AG R ++ S +V L F+ G T
Sbjct: 258 EIAAAYDLLNTDRNNFNVHIWYNSTYKDDAGNRLIKLIRVPRSVNLVSNAYLQFLQGPGT 317
Query: 262 RVL 270
R+L
Sbjct: 318 RML 320
>At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4
other kinesin-like proteins of A. thaliana: F02P16.12
(PID:g2191180), katA (D11371), katB (D21137), and katC
(D21138); contains non-consensus AT-AC splice sites at
intron 10
Length = 1087
Score = 27.1 bits (57), Expect = 5.9
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +2
Query: 17 EASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYV 160
E + ++ +EV D G I+V +PF QE Q+ +GNI +
Sbjct: 362 EENRLLYNEVQDLKGTIRVYCRVRPFFQEQKDMQSTVDYIGENGNIII 409
>At5g14770.1 68418.m01733 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 938
Score = 26.6 bits (56), Expect = 7.7
Identities = 16/67 (23%), Positives = 31/67 (46%)
Frame = +2
Query: 26 NVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAM 205
NV++SE ++ ++ L K + +SP + ISG + N+K+ +
Sbjct: 822 NVLISEFANVGKMLQARELLKEMGKRGVSPNTSTYCTMISGLCKLCTHPDVEWNKKAMYL 881
Query: 206 TKAQELL 226
+A+ LL
Sbjct: 882 AEAKGLL 888
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,660,978
Number of Sequences: 28952
Number of extensions: 157856
Number of successful extensions: 481
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 464
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 481
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -