Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00507
(656 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 111 6e-26
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 48 1e-06
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 38 0.001
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 36 0.007
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 32 0.063
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 31 0.15
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 28 1.4
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.4
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 1.8
SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c... 25 7.3
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 25 9.6
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 111 bits (268), Expect = 6e-26
Identities = 60/164 (36%), Positives = 84/164 (51%), Gaps = 1/164 (0%)
Frame = +1
Query: 22 MATDNLTALLYKPNDLRLVQTPIPEISDD-EVLLRMDCVGICGSDVHYWQKGQCGHFVLE 198
MA +L K D + P ++DD +V + + GICGSDVHYW++G G F+L+
Sbjct: 1 MAPAEKAFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILK 60
Query: 199 EPMIMGHEASGVVAKIGSKSRI*QLVIEWP*SPACRVGTASSVRQDDTTYAPT*SSAPRP 378
+PMI+GHE++GVV ++G + P C R P A P
Sbjct: 61 KPMILGHESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATP 120
Query: 379 QLHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLAVGIHACKR 510
G L YY DFC KLP +++EEGAL EP++V +HA R
Sbjct: 121 PYDGTLRTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTR 164
Score = 92.7 bits (220), Expect = 4e-20
Identities = 50/133 (37%), Positives = 70/133 (52%), Gaps = 1/133 (0%)
Frame = +3
Query: 255 VKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATPPVTRKPRQILQARSRFLLQ 434
V +L GD VA+EPG CR C++C++GRY+LCP M F ATPP R F +
Sbjct: 80 VSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPYDGTLRTYYITTEDFCTK 139
Query: 435 AS*SRDHGRRSATGAPRG-GHSRVQEGGVSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVL 611
+ A P + G + G VLV+G G +GLL M AKA+GA ++
Sbjct: 140 LP-KQISVEEGALFEPMSVAVHAMTRGNLKCGSRVLVMGCGTVGLLMMAVAKAYGAIDIV 198
Query: 612 IIDILQSRLDFAK 650
+D SR++FA+
Sbjct: 199 AVDASPSRVEFAQ 211
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 48.0 bits (109), Expect = 1e-06
Identities = 24/67 (35%), Positives = 40/67 (59%), Gaps = 2/67 (2%)
Frame = +1
Query: 58 PNDLRLVQTPIPEISDDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEASG 231
P +++ + P+ E DEVL+ + G+C +D+H Q G + L + P+I GHE +G
Sbjct: 18 PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAG 73
Query: 232 VVAKIGS 252
VV K+G+
Sbjct: 74 VVVKVGA 80
Score = 30.7 bits (66), Expect = 0.19
Identities = 35/136 (25%), Positives = 56/136 (41%), Gaps = 3/136 (2%)
Frame = +3
Query: 255 VKNLTVGDRVAIE-PGVPCRYCEFCKTGRYHLCPDMIFCA-TPPVTRKPRQILQARSRFL 428
V L +GDRV ++ C CE+C +CP + T T + I A +
Sbjct: 82 VTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHIQLSGYTVDGTFQHYCIANATHATI 141
Query: 429 LQAS*SRDHGRRSATGAPRGGHSRVQEGGVSAGHVVLVLGA-GPIGLLTMLTAKAFGAHK 605
+ S + A + ++E V G + + GA G +G L + AKA A +
Sbjct: 142 IPESVPLEVA-APIMCAGITCYRALKESKVGPGEWICIPGAGGGLGHLAVQYAKAM-AMR 199
Query: 606 VLIIDILQSRLDFAKS 653
V+ ID + + KS
Sbjct: 200 VVAIDTGDDKAELVKS 215
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 38.3 bits (85), Expect = 0.001
Identities = 16/33 (48%), Positives = 18/33 (54%)
Frame = +3
Query: 252 EVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 350
EV NL +GDRV I + C C FCK Y C
Sbjct: 109 EVNNLEIGDRVVIAFDLACGQCSFCKRHEYAAC 141
Score = 37.9 bits (84), Expect = 0.001
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = +3
Query: 510 GGVSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILQSRLDFAK 650
G V G V + G GPIGL A+ GA KV+ I+++ R++ A+
Sbjct: 217 GEVKKGDTVAIWGMGPIGLYAGRWAQILGASKVIGIEVVPERIELAR 263
Score = 36.3 bits (80), Expect = 0.004
Identities = 21/68 (30%), Positives = 39/68 (57%), Gaps = 2/68 (2%)
Frame = +1
Query: 58 PNDLRLVQTPIPEISDD-EVLLRMDCVGIC-GSDVHYWQKGQCGHFVLEEPMIMGHEASG 231
P ++++ + P P I+ +V+++ IC GSD H + G +E+ I+GHE+ G
Sbjct: 45 PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCG 101
Query: 232 VVAKIGSK 255
+VA+ G +
Sbjct: 102 IVAEKGDE 109
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 35.5 bits (78), Expect = 0.007
Identities = 20/59 (33%), Positives = 29/59 (49%)
Frame = +3
Query: 474 GAPRGGHSRVQEGGVSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILQSRLDFAK 650
G G + V +G V V+G G +GL M A A GA +++ IDI + +AK
Sbjct: 177 GVTTGFGAVTHSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAK 235
Score = 31.5 bits (68), Expect = 0.11
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 255 VKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 350
V N+ GD V + C+ C+FC++G+ +LC
Sbjct: 82 VINVRPGDHVILLYTPECKECKFCRSGKTNLC 113
Score = 28.3 bits (60), Expect = 1.0
Identities = 15/47 (31%), Positives = 25/47 (53%)
Frame = +1
Query: 109 EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVVAKIG 249
EV +++D +C +D Y G P+++GHE +G+V IG
Sbjct: 36 EVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIG 79
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 32.3 bits (70), Expect = 0.063
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3
Query: 252 EVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 350
+V + VGD V C+ C+FCK+G+ +LC
Sbjct: 84 QVTTVQVGDPVIALYTPECKTCKFCKSGKTNLC 116
Score = 29.1 bits (62), Expect = 0.59
Identities = 19/64 (29%), Positives = 33/64 (51%)
Frame = +1
Query: 58 PNDLRLVQTPIPEISDDEVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVV 237
P + VQ P + EV +++ G+C +D Y G+ + P+I+GHE +G+V
Sbjct: 24 PLSIENVQVFPPRVH--EVRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIV 78
Query: 238 AKIG 249
+G
Sbjct: 79 ESVG 82
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 31.1 bits (67), Expect = 0.15
Identities = 19/51 (37%), Positives = 27/51 (52%)
Frame = +3
Query: 504 QEGGVSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILQSRLDFAKSL 656
+E V G+ VLVLG G + + A A GA+ V + +L+FAK L
Sbjct: 159 KEHQVKPGNNVLVLGTGGVSTFALQFALAAGAN-VTVTSSSDEKLEFAKKL 208
>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 204
Score = 27.9 bits (59), Expect = 1.4
Identities = 15/46 (32%), Positives = 18/46 (39%)
Frame = -2
Query: 604 LCAPKALAVSMVRRPMGPAPSTSTTCPAETPPSCTRECPPRGAPVA 467
L P +V P GP PS S + TP + P AP A
Sbjct: 45 LTIPPPPSVDHSAPPSGPPPSYSNSAAPATPAASASSAAPAPAPAA 90
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.4
Identities = 15/32 (46%), Positives = 16/32 (50%)
Frame = -2
Query: 562 PMGPAPSTSTTCPAETPPSCTRECPPRGAPVA 467
P+G A TST P TPPS PP P A
Sbjct: 405 PLGNASRTSTP-PVPTPPSLPPSAPPSLPPSA 435
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/51 (33%), Positives = 19/51 (37%), Gaps = 3/51 (5%)
Frame = -2
Query: 598 APKALAVSMVRRPMGPAPSTSTTCPAETPPSCTRECP---PRGAPVALLLP 455
AP + R P T + P PPS P P GAP A LP
Sbjct: 400 APALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLP 450
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.5 bits (58), Expect = 1.8
Identities = 16/46 (34%), Positives = 19/46 (41%), Gaps = 2/46 (4%)
Frame = -2
Query: 619 SIMSTLCAPKALAVSMVRRPMGPAPSTSTTCPAET--PPSCTRECP 488
S ST P + S P PSTST+C T PP+ P
Sbjct: 309 STSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTP 354
>SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 625
Score = 25.4 bits (53), Expect = 7.3
Identities = 23/70 (32%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Frame = -2
Query: 337 RPVLQNSQYRHGTPGSMATRS----PTVRFLTSNRSWPLLLKLRVP*SLVLPARSVRIDP 170
R +L+N Q R G+ TRS P +RF N + PLL RV VL I
Sbjct: 338 RYILENGQSRPDRTGT-GTRSVFAPPQLRFSLRNNTLPLLTTKRVFLRGVLEELLWFIHG 396
Query: 169 SASSGHLTRR 140
++ HL+ +
Sbjct: 397 DTNANHLSEK 406
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 25.0 bits (52), Expect = 9.6
Identities = 17/67 (25%), Positives = 25/67 (37%), Gaps = 2/67 (2%)
Frame = +3
Query: 336 RYHLCPDMIFCATPPVTRKPRQILQARSRFLLQAS*SRDHGRRSATGAPRGGHSRVQEGG 515
R H P TP + +A+S + A G S GG++R + G
Sbjct: 12 RAHAVPSQDATTTPDNANNVPKEPRAQSMISIAADDLNQEGEMSDDNQQEGGNNRTSQNG 71
Query: 516 V--SAGH 530
S+GH
Sbjct: 72 TSGSSGH 78
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,830,061
Number of Sequences: 5004
Number of extensions: 57257
Number of successful extensions: 202
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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