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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00497X
         (574 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g51690.1 68416.m05668 hypothetical protein low similarity to ...    32   0.31 
At3g53540.1 68416.m05912 expressed protein                             29   1.7  
At5g22940.1 68418.m02682 exostosin family protein contains Pfam ...    29   2.2  
At2g02680.1 68415.m00207 DC1 domain-containing protein contains ...    28   5.1  
At1g13180.1 68414.m01528 actin-related protein 3 (ARP3) identica...    28   5.1  
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    27   6.7  
At5g05510.1 68418.m00598 protein kinase-related low similarity t...    27   8.9  
At1g42460.1 68414.m04896 Ulp1 protease family protein contains P...    27   8.9  

>At3g51690.1 68416.m05668 hypothetical protein low similarity to
           SP|P07271 DNA repair and recombination protein PIF1,
           mitochondrial precursor {Saccharomyces cerevisiae}
          Length = 374

 Score = 31.9 bits (69), Expect = 0.31
 Identities = 17/56 (30%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
 Frame = +1

Query: 94  IRQLIDEALGEYACQAYNGEGSPATLLMEVRAYKQDDTPSDNKYLVS-RPGEGVQC 258
           I+ L+ E  GEY  ++YN +    + ++    ++ DD    N Y++S  PGE  +C
Sbjct: 96  IKTLLKEVYGEYFAKSYNPDFCHDSAIL---CHRDDDVDQINDYMLSLLPGEEKEC 148


>At3g53540.1 68416.m05912 expressed protein
          Length = 924

 Score = 29.5 bits (63), Expect = 1.7
 Identities = 19/64 (29%), Positives = 30/64 (46%)
 Frame = -1

Query: 553 SAYRQTGNTSSTYPYLSPDAILTPSLDQYTFSGCGYPSTSQGRLSSAPAVSVGGADNILV 374
           S  R   N S +    SP+  +TPSL ++ +   G PS S     +  + S G A++   
Sbjct: 577 SKSRPGSNKSHSSYNSSPEVSITPSLSKFVYMNDGIPSKSASPFKARSSFS-GDANSDTE 635

Query: 373 DTGA 362
           D+ A
Sbjct: 636 DSSA 639


>At5g22940.1 68418.m02682 exostosin family protein contains Pfam
           profile: PF03016 exostosin family
          Length = 469

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 11/37 (29%), Positives = 18/37 (48%)
 Frame = -3

Query: 266 HYPHWTPSPGRDTRYLLSEGVSSCLYARTSISNVAGL 156
           HYP W  S G D  ++ S    +C +A   ++   G+
Sbjct: 198 HYPFWNRSQGSDHVFVASHDFGACFHAMEDMAIEEGI 234


>At2g02680.1 68415.m00207 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 649

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = +1

Query: 46  VPYSSTLYEARENVLLIRQLIDEALGEYACQAYNG 150
           + ++S+L + + +  + RQ +D   G Y C A NG
Sbjct: 290 ISFTSSLLKGKWSCGVCRQEVDRKYGAYTCNACNG 324


>At1g13180.1 68414.m01528 actin-related protein 3 (ARP3) identical
           to actin-related protein 3 (ARP3) [Arabidopsis thaliana]
           GI:21427461; contains Pfam profile PF00022: Actin
          Length = 427

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 15/26 (57%), Positives = 16/26 (61%)
 Frame = -2

Query: 87  NVFSGFVQCTAVWHHGTVKSSVPEDF 10
           NV S  VQ  AVW  G+V SS PE F
Sbjct: 375 NVVSHPVQRFAVWFGGSVLSSTPEFF 400


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
           identical to ubiquitin-protein ligase 2 [Arabidopsis
           thaliana] GI:7108523; E3, HECT-domain protein family;
           similar to ubiquitin-protein ligase 2 GI:7108523 from
           [Arabidopsis thaliana]
          Length = 3658

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 23/62 (37%), Positives = 27/62 (43%), Gaps = 5/62 (8%)
 Frame = +1

Query: 10  EIFWYRGLNGP-----MVPYSSTLYEARENVLLIRQLIDEALGEYACQAYNGEGSPATLL 174
           EI    G NGP      +PYS  L      +LL   L   +LG YA    N  GS  +LL
Sbjct: 462 EIVCCSGSNGPEDDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLYGSEESLL 521

Query: 175 ME 180
            E
Sbjct: 522 PE 523


>At5g05510.1 68418.m00598 protein kinase-related low similarity to
           SP|O60566 Mitotic checkpoint serine/threonine-protein
           kinase BUB1 beta (EC 2.7.1.-) {Homo sapiens}
          Length = 471

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 14/29 (48%), Positives = 16/29 (55%)
 Frame = +2

Query: 320 HHSSRSRFQRSPVYRAGVDKDIVGAPDAD 406
           HH    R Q  PV    VDK IVG P+A+
Sbjct: 172 HHKVDERQQHEPVLNF-VDKAIVGKPEAE 199


>At1g42460.1 68414.m04896 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain
          Length = 762

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +1

Query: 25  RGLNGPMVPYSSTLYEARENVLLIRQLID 111
           RG  G  +PY STL+   E+V + R  +D
Sbjct: 113 RGTGGKQMPYYSTLFGLEEDVTVERAHLD 141


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,418,909
Number of Sequences: 28952
Number of extensions: 210703
Number of successful extensions: 738
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 738
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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