BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00489 (772 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 96 6e-21 SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 42 7e-05 SPCC4G3.07c |phf1|swp1, saf50|PHD finger containing protein Phf1... 29 0.74 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 1.3 SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 27 2.2 SPAPB2B4.03 |cig2|cyc17|cyclin Cig2|Schizosaccharomyces pombe|ch... 27 3.0 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 5.2 SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 26 5.2 SPAC26H5.05 |||IPT/TIG ankyrin repeat protein|Schizosaccharomyce... 26 5.2 >SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 95.9 bits (228), Expect = 6e-21 Identities = 42/86 (48%), Positives = 64/86 (74%), Gaps = 2/86 (2%) Frame = +1 Query: 511 NMDDFKRVYSNED-EAKSIPYFWEKFDPENYSIWYAEYKYPEELAK-VFMSCNLITGMFQ 684 +++++KRVYSN+D + ++P+F+E FDPENYS+W +Y YPE+L + VFM+ NLI G FQ Sbjct: 264 DIEEYKRVYSNQDTRSGALPWFFEHFDPENYSVWKVDYSYPEDLKQPVFMTNNLIGGFFQ 323 Query: 685 RLDKMRKQAFASVCLFGEDNNSTISG 762 RL+ RK F + GE+ ++TI+G Sbjct: 324 RLEASRKYIFGCCVVIGENGDNTITG 349 Score = 33.9 bits (74), Expect = 0.026 Identities = 17/69 (24%), Positives = 35/69 (50%) Frame = +3 Query: 45 FNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRS 224 +++Q + A++ + D L ++T+LV R+TLAD+ L +VL S + Sbjct: 121 YDEQAAKEAETAIALIFARFDEELASKTYLVGSRLTLADIFFTCFLKFGATYVLTKSYLA 180 Query: 225 SLINVQRWF 251 ++ R++ Sbjct: 181 KYTHIYRYY 189 >SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 220 Score = 42.3 bits (95), Expect = 7e-05 Identities = 21/80 (26%), Positives = 44/80 (55%) Frame = +3 Query: 15 WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAF 194 WV + G + + ++ + + + + +LK+ + + RT+LV +R TLAD+ S L F Sbjct: 116 WVGMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFF 175 Query: 195 QHVLDPSVRSSLINVQRWFL 254 ++D R L ++ R+++ Sbjct: 176 NSIIDEKTRKELPHLTRYYI 195 >SPCC4G3.07c |phf1|swp1, saf50|PHD finger containing protein Phf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 461 Score = 29.1 bits (62), Expect = 0.74 Identities = 18/53 (33%), Positives = 25/53 (47%) Frame = +2 Query: 572 SGRSLTPRTIPFGMPNTNTLRNSLRCS*AVTLLRVCSRG*TKCANRLSHPSAC 730 SGR + R + + PN L+ R VTL VC RG + +NR+ C Sbjct: 163 SGRKIQ-RPVAYN-PNATALKRKSRKVDMVTLCSVCQRGHSPLSNRIVFCDGC 213 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 28.3 bits (60), Expect = 1.3 Identities = 13/31 (41%), Positives = 17/31 (54%) Frame = +1 Query: 79 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 171 TY PP Y D+FS APS+ + S+S Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287 >SPBC36.10 |||mitochondrial intermembrane space protein sorting protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 27.5 bits (58), Expect = 2.2 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -3 Query: 767 PHPEMVELLSSPNRQTDAKACLRILSNL 684 PHPE+ + + +A AC++ LSNL Sbjct: 113 PHPELEATVFQQEARIEALACMKRLSNL 140 >SPAPB2B4.03 |cig2|cyc17|cyclin Cig2|Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 27.1 bits (57), Expect = 3.0 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = -3 Query: 488 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYL 381 RRGPWTP ++ + +L +++ L +H L Sbjct: 324 RRGPWTPKLVESSGGYEEHELKEIAYIMLHYHNKPL 359 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 26.2 bits (55), Expect = 5.2 Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 4/41 (9%) Frame = -3 Query: 770 HPH-PEMVELLSS---PNRQTDAKACLRILSNLWNIPVIRL 660 +PH P V+ L+ PN + CL IL WN PV+ L Sbjct: 80 YPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWN-PVLNL 119 >SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 964 Score = 26.2 bits (55), Expect = 5.2 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +2 Query: 491 SMPKGTSTWMISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPNTN 625 S P +ST+ S+V LN + G+ + TIP G P+T+ Sbjct: 204 SQPVPSSTFSRSAVVPNPSLPLNPSVLQGQVMNNPTIPKGTPSTS 248 >SPAC26H5.05 |||IPT/TIG ankyrin repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1151 Score = 26.2 bits (55), Expect = 5.2 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +2 Query: 518 MISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPNTNTLRNSLRC 649 +++ +TP + +L SGRSL T G+ N +T + C Sbjct: 843 LVTDTTTPYLSDFSLVNESGRSLLHLTAACGLSNASTFLCNAGC 886 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,094,598 Number of Sequences: 5004 Number of extensions: 62071 Number of successful extensions: 179 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 371330890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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