Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00489
(772 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 72 4e-13
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 72 4e-13
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 36 0.022
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 33 0.16
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 32 0.48
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 30 1.9
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 29 2.6
At2g19280.1 68415.m02250 pentatricopeptide (PPR) repeat-containi... 29 2.6
At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 29 4.5
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 29 4.5
At1g26360.1 68414.m03216 hydrolase, alpha/beta fold family prote... 28 5.9
At4g14096.1 68417.m02176 F-box family protein contains F-box dom... 28 7.9
At3g28910.1 68416.m03608 myb family transcription factor (MYB30)... 28 7.9
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 72.1 bits (169), Expect = 4e-13
Identities = 31/86 (36%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
Frame = +1
Query: 514 MDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEELAKVFMSCNLITGMFQ 684
+DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E F++ N + G Q
Sbjct: 267 LDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQ 326
Query: 685 RLDKMRKQAFASVCLFGEDNNSTISG 762
R+D RK +F + + G + + G
Sbjct: 327 RMDLARKYSFGKMLICGSEGPFKVKG 352
Score = 50.4 bits (115), Expect = 1e-06
Identities = 27/79 (34%), Positives = 40/79 (50%)
Frame = +3
Query: 15 WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAF 194
W P +G F+ E A S L L+ L+ HL + TFLV +TLAD++ L F
Sbjct: 112 WFAPRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGF 171
Query: 195 QHVLDPSVRSSLINVQRWF 251
V+ S+ +V+R+F
Sbjct: 172 ATVMTKKFTSAFPHVERYF 190
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 72.1 bits (169), Expect = 4e-13
Identities = 31/86 (36%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
Frame = +1
Query: 514 MDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEELAKVFMSCNLITGMFQ 684
+DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E F++ N + G Q
Sbjct: 268 LDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQ 327
Query: 685 RLDKMRKQAFASVCLFGEDNNSTISG 762
R+D RK +F + + G + + G
Sbjct: 328 RMDLARKYSFGKMLICGSEGPFKVKG 353
Score = 51.2 bits (117), Expect = 7e-07
Identities = 29/79 (36%), Positives = 40/79 (50%)
Frame = +3
Query: 15 WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAF 194
W P +G M ++ E A S L AL L+ HL + T+LV ITLAD+I L F
Sbjct: 112 WFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGF 171
Query: 195 QHVLDPSVRSSLINVQRWF 251
V+ S +V+R+F
Sbjct: 172 ATVMTKKFTSEFPHVERYF 190
>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
domain-containing protein contains Pfam domain PF00043:
Glutathione S-transferase, C-terminal domain
Length = 399
Score = 36.3 bits (80), Expect = 0.022
Identities = 16/46 (34%), Positives = 31/46 (67%)
Frame = +3
Query: 48 NKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLL 185
+++ + A ++L + L ++ HL + +L ER+TLADV +F+TL+
Sbjct: 261 SQEAYDGAVNELFSTLDEIEDHLGSNRYLCGERLTLADVCLFTTLI 306
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 33.5 bits (73), Expect = 0.16
Identities = 19/55 (34%), Positives = 28/55 (50%)
Frame = +3
Query: 87 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWF 251
+ LK LD HLLTR+++ + + D+ VF+ L S +NV RWF
Sbjct: 11 SGLKKLDEHLLTRSYITGYQASKDDITVFT--------ALSKPPTSEFVNVSRWF 57
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 31.9 bits (69), Expect = 0.48
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +3
Query: 87 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWF 251
A LK LD HLLTR+++ + + D+ VF+ L P+ S +N RW+
Sbjct: 11 AGLKKLDEHLLTRSYITGYQASKDDITVFAAL------AKPPT--SQYVNASRWY 57
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = +2
Query: 497 PKGTSTWM-ISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPNT-NTLRNSL 643
P+ W + + PT L ++L G S+TP IPF T +RN++
Sbjct: 552 PQAIQGWTSVGHEAAPTTITLTIYLDDGGSVTPVYIPFRSDQTVRDIRNAI 602
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 29.5 bits (63), Expect = 2.6
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +1
Query: 31 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRES 150
L++ + N N +S + WPP K W D H + E+
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEA 234
>At2g19280.1 68415.m02250 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 693
Score = 29.5 bits (63), Expect = 2.6
Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 159 DVIVFSTLLHAFQHVLDPSVRSSLIN--VQRWFLPSPTSH 272
DV++++TL+H + V D LI VQR LP+ ++H
Sbjct: 617 DVVLYNTLIHGYCSVGDIEKACELIGLMVQRGMLPNESTH 656
>At5g07270.1 68418.m00829 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 513
Score = 28.7 bits (61), Expect = 4.5
Identities = 20/50 (40%), Positives = 23/50 (46%), Gaps = 7/50 (14%)
Frame = +1
Query: 64 NVQSLTYWPP*-------KYWTDIFSHAPSLLPRESHLPMSLSSVHCCML 192
NV S+T PP ++ F PS L RE LPMSL CML
Sbjct: 344 NVPSVTVGPPGSIPCPLCRHGITAFKRLPSSLTREMKLPMSLGFCAPCML 393
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 28.7 bits (61), Expect = 4.5
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = -1
Query: 235 FISDERTLGSSTCWKACSSVLKTMTSASVILSVTRKVRVRRCPS 104
F+ ++ +C K CSS LKT+ S + S ++ +C S
Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVS 199
>At1g26360.1 68414.m03216 hydrolase, alpha/beta fold family protein
similar to SP|Q40708 PIR7A protein {Oryza sativa},
ethylene-induced esterase [Citrus sinensis] GI:14279437,
polyneuridine aldehyde esterase [Rauvolfia serpentina]
GI:6651393; contains Pfam profile PF00561: alpha/beta
hydrolase fold
Length = 444
Score = 28.3 bits (60), Expect = 5.9
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +2
Query: 491 SMPKGTSTWMISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPN 619
+ P GTS+ +S+V TP K+ +S T P PN
Sbjct: 130 AQPSGTSSGPVSAVQTPKKSSAGFVRSSSSRQRSSTDPMIKPN 172
>At4g14096.1 68417.m02176 F-box family protein contains F-box domain
Pfam:PF00646
Length = 468
Score = 27.9 bits (59), Expect = 7.9
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 512 TWMISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPN 619
TW +SVS PT +L F T R P+++ PN
Sbjct: 199 TWDFASVSVPTLKRLT-FSTQVRDEFPKSVSIDTPN 233
>At3g28910.1 68416.m03608 myb family transcription factor (MYB30)
identical to myb-like protein GB:AJ007289 [Arabidopsis
thaliana] (Plant J. 20 (1), 57-66 (1999))
Length = 323
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
Frame = -3
Query: 488 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYLFCPPSCSV---HQLTPG 339
++GPWTP QE P +W A+ +T L C SC + + L PG
Sbjct: 13 KKGPWTPEEDIILVTYIQEH-GPGNWRAVPTNTGLLRCSKSCRLRWTNYLRPG 64
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,727,977
Number of Sequences: 28952
Number of extensions: 351164
Number of successful extensions: 981
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 978
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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