Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00484X
(366 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B) 100 3e-22
At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B) 100 3e-22
At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A) ribo... 100 3e-22
At1g53920.1 68414.m06138 GDSL-motif lipase/hydrolase family prot... 30 0.54
At5g04110.1 68418.m00397 DNA topoisomerase II family protein sii... 28 2.2
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 2.9
At1g63670.1 68414.m07205 expressed protein 27 3.8
At4g02020.1 68417.m00272 zeste-like protein 1 (EZA1) identical t... 27 5.0
At5g07900.1 68418.m00912 mitochondrial transcription termination... 26 8.8
At3g58240.1 68416.m06493 meprin and TRAF homology domain-contain... 26 8.8
At2g34240.1 68415.m04189 hypothetical protein contains Pfam prof... 26 8.8
At1g71220.1 68414.m08219 UDP-glucose:glycoprotein glucosyltransf... 26 8.8
At1g13460.2 68414.m01575 serine/threonine protein phosphatase 2A... 26 8.8
At1g13460.1 68414.m01574 serine/threonine protein phosphatase 2A... 26 8.8
>At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 100 bits (240), Expect = 3e-22
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +1
Query: 40 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 219
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59
Query: 220 SNKKTRHMLPNG 255
S+KKTRH LPNG
Sbjct: 60 SDKKTRHYLPNG 71
Score = 40.3 bits (90), Expect = 4e-04
Identities = 18/38 (47%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = +3
Query: 240 YAPKWFRKVLVHNVKELEI**C-KQEYCAEIAHGVSSR 350
Y P F+K +VHN ELE+ + YCAEIAH +S++
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTK 104
>At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 100 bits (240), Expect = 3e-22
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +1
Query: 40 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 219
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59
Query: 220 SNKKTRHMLPNG 255
S+KKTRH LPNG
Sbjct: 60 SDKKTRHYLPNG 71
Score = 40.3 bits (90), Expect = 4e-04
Identities = 18/38 (47%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = +3
Query: 240 YAPKWFRKVLVHNVKELEI**C-KQEYCAEIAHGVSSR 350
Y P F+K +VHN ELE+ + YCAEIAH +S++
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTK 104
>At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A)
ribosomal protein L32, human, PIR1:R5HU32
Length = 133
Score = 100 bits (240), Expect = 3e-22
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +1
Query: 40 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 219
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59
Query: 220 SNKKTRHMLPNG 255
S+KKTRH LPNG
Sbjct: 60 SDKKTRHYLPNG 71
Score = 40.3 bits (90), Expect = 4e-04
Identities = 19/38 (50%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = +3
Query: 240 YAPKWFRKVLVHNVKELEI**C-KQEYCAEIAHGVSSR 350
Y P F+K +VHN ELE+ + YCAEIAH VS++
Sbjct: 67 YLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTK 104
>At1g53920.1 68414.m06138 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich proteins
SP|P40603 SP|P40602 from {Arabidopsis thaliana};
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 385
Score = 29.9 bits (64), Expect = 0.54
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = +1
Query: 97 IRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPNGSV 261
+R Q D Y K++R WR G + +R + YL I GSN + L N S+
Sbjct: 146 LRTQLDHYKKVERLWRTNFGKEESKKRISRAVYL---ISIGSNDYSSIFLTNQSL 197
>At5g04110.1 68418.m00397 DNA topoisomerase II family protein
siimilar to DNA topoisomerase II subunit B [Thermotoga
maritima] GI:1622792; contains Pfam profiles PF00204:
DNA topoisomerase II (N-terminal region), PF00249:
Myb-like DNA-binding domain
Length = 546
Score = 27.9 bits (59), Expect = 2.2
Identities = 25/71 (35%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = +1
Query: 100 RHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPNGSVRS*FTM 279
+H SDR K KR W +P D+ V G+ G KK++ P SV S
Sbjct: 252 KHDSDRCFKNKRTWEQPWS-DDAVSTA-PGESSENTSGSSFEKKSKK--PKSSVSSSHLC 307
Query: 280 LKSWKS-DDAN 309
S +S DDAN
Sbjct: 308 FSSRQSADDAN 318
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 27.5 bits (58), Expect = 2.9
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +1
Query: 43 AIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPR 153
A+ P ++ + +K +++H + R DKL++ KPR
Sbjct: 194 AVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPR 230
>At1g63670.1 68414.m07205 expressed protein
Length = 689
Score = 27.1 bits (57), Expect = 3.8
Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 5/63 (7%)
Frame = +1
Query: 145 KPR---GIDNRVRRRFKGQYLMPNIGYGSNKKTRHM--LPNGSVRS*FTMLKSWKSDDAN 309
KPR +D++V RFK + GSNK + H P GS R ++++ D +
Sbjct: 261 KPRLSSSVDDKVYLRFKHLAKKLKLVVGSNKDSNHAENKPEGSSRGREIEAAAFRTSDVS 320
Query: 310 RST 318
ST
Sbjct: 321 TST 323
>At4g02020.1 68417.m00272 zeste-like protein 1 (EZA1) identical to
enhancer of zeste-like protein 1(EZA1) (GI:4185507)
[Arabidopsis thaliana]; similar to polycomb group
[Arabidopsis thaliana] GI:1903019 (curly leaf); contains
Pfam profile PF00856: SET domain
Length = 856
Score = 26.6 bits (56), Expect = 5.0
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +3
Query: 27 RNIQDGYKTCLQADNRQKEDE 89
RN+ G KTCL N +E+E
Sbjct: 517 RNLLSGLKTCLDVSNYMRENE 537
>At5g07900.1 68418.m00912 mitochondrial transcription termination
factor family protein / mTERF family protein low
similarity to SP|Q99551 Transcription termination
factor, mitochondrial precursor (mTERF) {Homo sapiens};
contains Pfam profile PF02536: mTERF
Length = 405
Score = 25.8 bits (54), Expect = 8.8
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 4/47 (8%)
Frame = -1
Query: 147 FTPIPLKFVIAIRLMPDKSLRPLFDDCRPVNR----SYSHLVCFFKK 19
F P FV+AI + K + ++D C V + S ++C FKK
Sbjct: 256 FNPQKSTFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKK 302
>At3g58240.1 68416.m06493 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 317
Score = 25.8 bits (54), Expect = 8.8
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = -1
Query: 141 PIPLKFVIAIRLMPDKSLRPLFDDCRPVNRSYSHLVCFFKKRD 13
P+ KF I+ S +P++ D + + H++ + KKRD
Sbjct: 4 PVDNKFTWVIKNFCSVSPKPIYSDQFLIGGNKWHILAYSKKRD 46
>At2g34240.1 68415.m04189 hypothetical protein contains Pfam
profiles PF04780: Protein of unknown function (DUF629),
PF04781: Protein of unknown function (DUF627)
Length = 712
Score = 25.8 bits (54), Expect = 8.8
Identities = 14/57 (24%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 85 TKRFIRHQSDRY-DKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPN 252
+++ I + ++Y DK+K ++ K + N +R R+ ++ + Y RH+L N
Sbjct: 216 SRKLIDYVENKYVDKVKEDFGKCVSVANNLRWRYWKCHICSQVYYCFTDCQRHILDN 272
>At1g71220.1 68414.m08219 UDP-glucose:glycoprotein
glucosyltransferase, putative similar to
UDP-glucose:glycoprotein glucosyltransferase precursor
GB:Q09332 [SP|Q09332] from Drosophila melanogaster,
[gi:7670746] and [gi:11346464] from Homo sapiens
Length = 1673
Score = 25.8 bits (54), Expect = 8.8
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +2
Query: 83 GRRDLSGINRIAMTNLRGIGVNLEVL 160
G +D S + RI + +LRG V+LEV+
Sbjct: 1253 GSQDQSSLKRITIDDLRGKVVHLEVV 1278
>At1g13460.2 68414.m01575 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit B', putative similar to
SWISS-PROT:Q28653 serine/threonine protein phosphatase
2A, 56 kDa regulatory subunit, delta isoform (PP2A, B
subunit, B' delta isoform, PP2A, B subunit, B56 delta
isoform, PP2A, B subunit, PR61 delta isoform, PP2A, B
subunit, R5 delta isoform, PP2A, B subunit, B'-gamma)
[Oryctolagus cuniculus]; contains Pfam domain, PF01603:
Protein phosphatase 2A regulatory B subunit (B56 family)
Length = 492
Score = 25.8 bits (54), Expect = 8.8
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +3
Query: 168 SPQAIQGSILDAQHRLRFQQEDPSYAPKWFRKVLVHNV 281
+PQ + ++DA L ++E+PS P W LV+ +
Sbjct: 153 NPQPRENKVIDA---LDLEEEEPSMDPTWPHLQLVYEI 187
>At1g13460.1 68414.m01574 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit B', putative similar to
SWISS-PROT:Q28653 serine/threonine protein phosphatase
2A, 56 kDa regulatory subunit, delta isoform (PP2A, B
subunit, B' delta isoform, PP2A, B subunit, B56 delta
isoform, PP2A, B subunit, PR61 delta isoform, PP2A, B
subunit, R5 delta isoform, PP2A, B subunit, B'-gamma)
[Oryctolagus cuniculus]; contains Pfam domain, PF01603:
Protein phosphatase 2A regulatory B subunit (B56 family)
Length = 492
Score = 25.8 bits (54), Expect = 8.8
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +3
Query: 168 SPQAIQGSILDAQHRLRFQQEDPSYAPKWFRKVLVHNV 281
+PQ + ++DA L ++E+PS P W LV+ +
Sbjct: 153 NPQPRENKVIDA---LDLEEEEPSMDPTWPHLQLVYEI 187
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,676,067
Number of Sequences: 28952
Number of extensions: 150159
Number of successful extensions: 438
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 423
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 435
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 477939072
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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