Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00478
(702 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 122 4e-29
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 111 1e-25
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 47 2e-06
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.16
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.21
SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 30 0.28
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.1
SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 27 3.4
SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.4
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 27 3.4
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 4.5
SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 26 4.5
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 7.9
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.9
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 122 bits (295), Expect = 4e-29
Identities = 62/81 (76%), Positives = 72/81 (88%)
Frame = +1
Query: 256 RPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLA 435
RP+LI+K+ IPVVMAGIIAIYGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLA
Sbjct: 47 RPDLIVKNTIPVVMAGIIAIYGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLA 104
Query: 436 AGFAIGIVGDAGVRGTAQQPR 498
AGFAIGIVGDAGVRGTAQQPR
Sbjct: 105 AGFAIGIVGDAGVRGTAQQPR 125
Score = 62.5 bits (145), Expect = 6e-11
Identities = 25/37 (67%), Positives = 32/37 (86%)
Frame = +2
Query: 137 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAM 247
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAM 43
Score = 41.1 bits (92), Expect = 1e-04
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +3
Query: 510 MILILIFAEVLGLYGLIVAIYL 575
MILILIFAEVLGLYGLIVA+ L
Sbjct: 130 MILILIFAEVLGLYGLIVALLL 151
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 111 bits (266), Expect = 1e-25
Identities = 50/81 (61%), Positives = 66/81 (81%)
Frame = +1
Query: 256 RPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLA 435
RPE++MKS+IPVVM+GII +YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+A
Sbjct: 47 RPEIVMKSLIPVVMSGIIGVYGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVA 106
Query: 436 AGFAIGIVGDAGVRGTAQQPR 498
AG+AIG+VGD GV+ +Q R
Sbjct: 107 AGYAIGVVGDRGVQSFMRQDR 127
Score = 50.0 bits (114), Expect = 3e-07
Identities = 21/37 (56%), Positives = 27/37 (72%)
Frame = +2
Query: 137 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAM 247
PIY FFG G ++++FS LGA YGTA +G GIAA+
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAV 43
Score = 39.1 bits (87), Expect = 6e-04
Identities = 18/22 (81%), Positives = 20/22 (90%)
Frame = +3
Query: 510 MILILIFAEVLGLYGLIVAIYL 575
M+LILIFAEVLGLYGLIV + L
Sbjct: 132 MVLILIFAEVLGLYGLIVGLIL 153
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 47.2 bits (107), Expect = 2e-06
Identities = 23/83 (27%), Positives = 41/83 (49%), Gaps = 6/83 (7%)
Frame = +1
Query: 259 PELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQE--PANY----PLYKGFIHLGAGLAVG 420
P + K++I ++ ++AIY L++A++ + + + PA + Y GF G+ VG
Sbjct: 80 PRIKTKNLISIIFCEVVAIYSLIIAIVFSAKINDINPAGFYTKSHYYTGFALFWGGITVG 139
Query: 421 FSGLAAGFAIGIVGDAGVRGTAQ 489
L G +GI G + AQ
Sbjct: 140 LCNLICGVCVGITGSSAALADAQ 162
Score = 29.1 bits (62), Expect = 0.64
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = +2
Query: 155 FGVMGAASAIIFSALGAAYGTAKSGTGI 238
+G++G AS + F +GAA+G GT I
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSI 72
Score = 27.5 bits (58), Expect = 2.0
Identities = 10/23 (43%), Positives = 18/23 (78%)
Frame = +3
Query: 507 RMILILIFAEVLGLYGLIVAIYL 575
+++++ IF VLGL+GLIV + +
Sbjct: 169 KVLVVEIFGSVLGLFGLIVGLLI 191
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 31.1 bits (67), Expect = 0.16
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +1
Query: 280 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 459
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 460 GDAGVRGT 483
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.21
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -2
Query: 485 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 384
+VP P++P MP P+A P A AP NP
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase
Byr2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 659
Score = 30.3 bits (65), Expect = 0.28
Identities = 24/78 (30%), Positives = 36/78 (46%), Gaps = 5/78 (6%)
Frame = -2
Query: 575 QVDGDDKSVKTQYFSENKNKNHSTNNLGC*AVPR-----TPASPTMPMAKPAARPENPTA 411
Q +G++KS K FSE+ + NH + + P +P SPT ++ E T
Sbjct: 329 QSNGNEKSSKYNVFSESAHGNHQVLSFSPGSSPSFIEQPSPISPTSTTSEDTNTLEEDTD 388
Query: 410 KPAPKWMNPL*RG*LAGS 357
+ KW+ RG L GS
Sbjct: 389 DQSIKWI----RGALIGS 402
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.1
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -2
Query: 485 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 312
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 311 AIIPA 297
A PA
Sbjct: 479 APAPA 483
>SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating
transcription Rct1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 432
Score = 26.6 bits (56), Expect = 3.4
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 95 TGPKNCDDYLHTLTPRNYFCTDK 27
TG KNCDD+L +P + D+
Sbjct: 402 TGNKNCDDHLRDKSPERRYRYDR 424
>SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 281
Score = 26.6 bits (56), Expect = 3.4
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 3/31 (9%)
Frame = -2
Query: 581 CVQVDGDD-KSV--KTQYFSENKNKNHSTNN 498
C++VD +D K + K+QY +EN N N S NN
Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTN-SINN 262
>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 447
Score = 26.6 bits (56), Expect = 3.4
Identities = 16/59 (27%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
Frame = -2
Query: 584 FCVQVDGDDKSVKTQYFSENKNKNHSTNNLGC*--AVPRT-PASPTMPMAKPAARPENP 417
F Q+ + + + + SE++ ++ TN+ G ++P++ P SP M P++ P NP
Sbjct: 126 FNPQLPSNSNTNRFDFGSESQLSSNYTNDTGLSLLSLPKSVPHSPAMHKRTPSSDPNNP 184
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 393 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 280
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 629
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = +1
Query: 439 GFAIGIVGDAGVRGTAQQPRXXXXXXXXXXXXKYWVFTDLSSPS 570
G A+G G+ G R +++P Y+ F DLS P+
Sbjct: 520 GSAVGQNGNVGPRQISEEPMSIIFNLGISNNWAYYYFRDLSFPA 563
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 25.4 bits (53), Expect = 7.9
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2
Query: 95 TGPKNCDDYLHTL 57
TGP N DDY+H +
Sbjct: 369 TGPSNTDDYIHRI 381
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -2
Query: 485 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 396
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,090,602
Number of Sequences: 5004
Number of extensions: 65119
Number of successful extensions: 222
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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