Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00460
(739 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g07610.1 68418.m00872 F-box family protein similar to unknown... 30 1.8
At2g16160.1 68415.m01852 hypothetical protein includes At2g06610... 29 2.4
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 29 3.2
At3g59100.1 68416.m06589 glycosyl transferase family 48 protein ... 29 4.3
At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ... 29 4.3
At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ... 29 4.3
At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ... 29 4.3
At1g49630.3 68414.m05566 peptidase M16 family protein / insulina... 28 7.4
At1g49630.2 68414.m05565 peptidase M16 family protein / insulina... 28 7.4
At1g49630.1 68414.m05564 peptidase M16 family protein / insulina... 28 7.4
At1g23090.1 68414.m02887 sulfate transporter, putative similar t... 28 7.4
At3g19170.1 68416.m02434 peptidase M16 family protein / insulina... 27 9.8
At2g40116.1 68415.m04933 phosphoinositide-specific phospholipase... 27 9.8
>At5g07610.1 68418.m00872 F-box family protein similar to unknown
protein (emb|CAB85517.1)
Length = 420
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +2
Query: 401 SELRILQKYFPLNSWIPKYSLRLVKLQPLFHQMFHATYYHSTRLH 535
S LR+ + +SW KY++ L L F +M YY R++
Sbjct: 306 SNLRVYEMESNSSSWSVKYNVDLEPLAAAFPEMIRTEYYTDRRIY 350
>At2g16160.1 68415.m01852 hypothetical protein includes At2g06610,
At5g28266, At3g42620, At4g07696, At2g06690, At2g16160,
At2g05480, At2g12140, At1g45080, At2g16330
Length = 217
Score = 29.5 bits (63), Expect = 2.4
Identities = 20/68 (29%), Positives = 26/68 (38%)
Frame = +2
Query: 71 QFLTEDYANNGIELNNRFGDDASEKIPLKNLSKLPEFKIATQLPKDAEFSLFLPKHQEMA 250
QFL E IE+ N D K+P K + K F + Q + EF F +
Sbjct: 114 QFLPEYVIGALIEVGNLTKDYRGRKLPFKIMDKQKFFNVKHQNDQAFEFQRFFQRLTGPQ 173
Query: 251 NDFLAFSW 274
N SW
Sbjct: 174 NIIALVSW 181
>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
Membrane-associated protein Hem (Dhem-2)
(Swiss-Prot:P55162) [Drosophila melanogaster]; weak
similarity to Nck-associated protein 1 (NAP 1)
(p125Nap1) (Membrane-associated protein HEM-2)
(Swiss-Prot:P55161) [Rattus norvegicus]
Length = 1339
Score = 29.1 bits (62), Expect = 3.2
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 313 CAFARVNLNPQLFNYCYSVALMHRRDTRKVRVKNFAEVFPSKFLD 447
CAF RVNL + ++ DTRK+R + F + +F D
Sbjct: 202 CAFVRVNLFAEKVLEAVGPSIFLSADTRKLRNEGFLSPYHPRFPD 246
>At3g59100.1 68416.m06589 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1934
Score = 28.7 bits (61), Expect = 4.3
Identities = 10/24 (41%), Positives = 15/24 (62%), Gaps = 2/24 (8%)
Frame = +3
Query: 591 INLH--HYHWHLVYPFTANDLSIV 656
+NLH HY WH +P N++ +V
Sbjct: 715 MNLHIGHYQWHEFFPHATNNIGVV 738
>At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 453
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +2
Query: 167 KLPEFKIATQLPKDAEFSLFLPKHQEM-ANDFLAFSWMYQRTNYKICYRH 313
K+P++K ++ KD + LPK + DF + ++ +Q NY + RH
Sbjct: 241 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH 290
>At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +2
Query: 167 KLPEFKIATQLPKDAEFSLFLPKHQEM-ANDFLAFSWMYQRTNYKICYRH 313
K+P++K ++ KD + LPK + DF + ++ +Q NY + RH
Sbjct: 255 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH 304
>At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +2
Query: 167 KLPEFKIATQLPKDAEFSLFLPKHQEM-ANDFLAFSWMYQRTNYKICYRH 313
K+P++K ++ KD + LPK + DF + ++ +Q NY + RH
Sbjct: 255 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH 304
>At1g49630.3 68414.m05566 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 27.9 bits (59), Expect = 7.4
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = -3
Query: 518 DNRLRGTSGGITAAVSRA*VNT--WESKNLEGNTSAKFL 408
+NRLRG+ GI AA A +N W S+ + G + +FL
Sbjct: 773 ENRLRGSGQGIAAARMDAMLNVAGWMSEQMGGLSYLEFL 811
>At1g49630.2 68414.m05565 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 27.9 bits (59), Expect = 7.4
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = -3
Query: 518 DNRLRGTSGGITAAVSRA*VNT--WESKNLEGNTSAKFL 408
+NRLRG+ GI AA A +N W S+ + G + +FL
Sbjct: 773 ENRLRGSGQGIAAARMDAMLNVAGWMSEQMGGLSYLEFL 811
>At1g49630.1 68414.m05564 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 27.9 bits (59), Expect = 7.4
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = -3
Query: 518 DNRLRGTSGGITAAVSRA*VNT--WESKNLEGNTSAKFL 408
+NRLRG+ GI AA A +N W S+ + G + +FL
Sbjct: 773 ENRLRGSGQGIAAARMDAMLNVAGWMSEQMGGLSYLEFL 811
>At1g23090.1 68414.m02887 sulfate transporter, putative similar to
sulfate transporter [Arabidopsis thaliana] GI:2285885;
contains Pfam profiles PF00916: Sulfate transporter
family, PF01740: STAS domain
Length = 631
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/29 (41%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 392 PEKSEL-RILQKYFPLNSWIPKYSLRLVK 475
P +++L R Q FP+ W P+YS L+K
Sbjct: 39 PNRTKLIRAAQYIFPILQWCPEYSFSLLK 67
>At3g19170.1 68416.m02434 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = -3
Query: 518 DNRLRGTSGGITAAVSRA*VNT--WESKNLEGNTSAKFL 408
+NRLRG+ GI AA A +N W S+ + G + +FL
Sbjct: 774 ENRLRGSGHGIAAARMDAMLNIAGWMSEQMGGLSYLEFL 812
>At2g40116.1 68415.m04933 phosphoinositide-specific phospholipase C
family protein contains Pfam profile: PF00388
phosphatidylinositol-specific phospholipase C
Length = 613
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +3
Query: 135 LLRRYPSRTSANSQNLKLQLNYPRTLNSHCFYLNIKKWQMTSWRSHGCTR 284
LLR YP T NS N K + + T + N++ + + W HG R
Sbjct: 403 LLRIYPKGTRFNSSNYKPLIGW--THGAQMIAFNMQGYGKSLWLMHGMFR 450
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,346,691
Number of Sequences: 28952
Number of extensions: 351723
Number of successful extensions: 896
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 885
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 896
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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