BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00459 (766 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1A10.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 2.2 SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 27 2.9 SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 27 3.9 SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 26 5.1 SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 26 5.1 SPAC29E6.07 ||SPAC30.11|sequence orphan|Schizosaccharomyces pomb... 26 5.1 SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 26 6.8 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 25 9.0 SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.0 SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 9.0 SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||... 25 9.0 >SPAPB1A10.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 27.5 bits (58), Expect = 2.2 Identities = 18/74 (24%), Positives = 32/74 (43%), Gaps = 5/74 (6%) Frame = -1 Query: 703 DESVHFNTHLALVREYPGDIALVELRSDED-----THCGATAATGVVHVYSVAEMPVVEV 539 +E VH+NT+ +V E + + EL D D + T T H ++ +P + Sbjct: 212 EEPVHYNTYDGVVNEPNSSVIIEELDDDYDELNDPMNNNDTPITNSTHSAQMSNLPTHDS 271 Query: 538 SEASVHGPVVEKSS 497 + + G V S+ Sbjct: 272 MDIDMGGAVPSTST 285 >SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 27.1 bits (57), Expect = 2.9 Identities = 13/36 (36%), Positives = 20/36 (55%) Frame = +1 Query: 502 TSRQRARVRSLHSPQLQAFQLQNKREQHR*QPSHHS 609 T+ Q +++ LH QLQ+ Q Q K+ Q + P S Sbjct: 249 TAAQLQQLKQLHYRQLQSVQQQQKQHQQKKTPQSGS 284 >SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1|||Manual Length = 380 Score = 26.6 bits (56), Expect = 3.9 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +3 Query: 315 HAYIDDVFQKHKESAYVRPYTRSELENPGVQVRSVSVETPG 437 H + V KH E T++ELE +R ++TPG Sbjct: 56 HMGPEKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPG 96 >SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2|Schizosaccharomyces pombe|chr 2|||Manual Length = 659 Score = 26.2 bits (55), Expect = 5.1 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = -2 Query: 108 PVWQSNGTSRDSFFNVSSPAFH 43 P++QSNG + S +NV S + H Sbjct: 326 PLYQSNGNEKSSKYNVFSESAH 347 >SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 781 Score = 26.2 bits (55), Expect = 5.1 Identities = 15/38 (39%), Positives = 19/38 (50%) Frame = +1 Query: 229 HDPEHRYLDNLVLSLMRPQRCAIHSSTAGTPTSMTFSR 342 H P H L NL+ M P H S A +PTS + S+ Sbjct: 115 HKPHHSGLKNLLEKAMHPG----HKSNANSPTSESPSK 148 >SPAC29E6.07 ||SPAC30.11|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 116 Score = 26.2 bits (55), Expect = 5.1 Identities = 13/42 (30%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = -2 Query: 723 SPAF-RTVTNLSISIHILRWSENIQGTLRSSNFGVMKIRTVV 601 SP F +T T + IHI ++SE + L + + +RT++ Sbjct: 56 SPLFIKTTTRPTALIHITKYSEALASRLLKGHMTQLSLRTIL 97 >SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 688 Score = 25.8 bits (54), Expect = 6.8 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 5/44 (11%) Frame = +1 Query: 34 IDEMERWRRNVEEAIATGTVRLPNGQTRPL-----DIDTLGNML 150 ID + +W R+V E + GT+ L G T L D++ +G+M+ Sbjct: 236 IDALLQWTRSVAECLKNGTL-LKIGNTDSLSSDVMDVEDMGSMM 278 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 25.4 bits (53), Expect = 9.0 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = +1 Query: 190 SIHNNGHSFTAYMHDPEHRYLDNLV 264 SI+NNG +HD E Y+ L+ Sbjct: 162 SIYNNGKGIPIEIHDKEKIYIPELI 186 >SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 867 Score = 25.4 bits (53), Expect = 9.0 Identities = 10/27 (37%), Positives = 14/27 (51%) Frame = -1 Query: 712 QDSDESVHFNTHLALVREYPGDIALVE 632 +D DE + N L+ +YP DI E Sbjct: 217 EDEDEDNNNNNRWLLIEQYPSDIVAYE 243 >SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 780 Score = 25.4 bits (53), Expect = 9.0 Identities = 17/53 (32%), Positives = 22/53 (41%) Frame = +1 Query: 202 NGHSFTAYMHDPEHRYLDNLVLSLMRPQRCAIHSSTAGTPTSMTFSRSTRNLP 360 N F Y HDP R L + + A S T P+S+T+S S P Sbjct: 73 NASDFHKYSHDPFQR--------LFKAKGNASFSKTKSFPSSVTYSPSEETFP 117 >SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 25.4 bits (53), Expect = 9.0 Identities = 9/30 (30%), Positives = 16/30 (53%) Frame = +3 Query: 315 HAYIDDVFQKHKESAYVRPYTRSELENPGV 404 H +D+V ++H+ + P S LE G+ Sbjct: 474 HKMVDEVLERHRHRYEINPAFVSRLEQGGI 503 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,252,428 Number of Sequences: 5004 Number of extensions: 69145 Number of successful extensions: 258 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 251 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 258 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 367316502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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