Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00441
(371 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34555.1 68417.m04910 40S ribosomal protein S25, putative 70 4e-13
At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E) ribo... 69 1e-12
At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B) 69 1e-12
At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A) 67 3e-12
At5g64950.1 68418.m08170 mitochondrial transcription termination... 29 0.99
At1g14500.1 68414.m01719 ankyrin repeat family protein contains ... 29 1.3
At2g17250.1 68415.m01992 expressed protein weak similarity to Ri... 28 2.3
At5g55500.1 68418.m06912 beta-(1,2)-xylosyltransferase (XYLT) id... 27 4.0
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta... 27 4.0
At5g41380.1 68418.m05028 hypothetical protein 27 5.3
At5g27640.1 68418.m03311 eukaryotic translation initiation facto... 27 5.3
At2g23170.1 68415.m02768 auxin-responsive GH3 family protein sim... 27 5.3
At1g24706.1 68414.m03104 expressed protein 27 5.3
At5g54770.1 68418.m06822 thiazole biosynthetic enzyme, chloropla... 26 7.0
At1g48180.1 68414.m05378 expressed protein ; expression supporte... 26 7.0
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 26 9.2
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 26 9.2
At4g36670.1 68417.m05203 mannitol transporter, putative similar ... 26 9.2
At2g02810.1 68415.m00226 UDP-galactose/UDP-glucose transporter c... 26 9.2
>At4g34555.1 68417.m04910 40S ribosomal protein S25, putative
Length = 108
Score = 70.1 bits (164), Expect = 4e-13
Identities = 30/51 (58%), Positives = 42/51 (82%)
Frame = +2
Query: 107 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELREK 259
+NN VLFD+ TY+KL E P++KLITP+++S+RL++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQATYDKLLSEAPKFKLITPSILSDRLRINGSLARRAIRELMAK 87
Score = 29.1 bits (62), Expect = 0.99
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = +1
Query: 256 KGLIKQVVQHHGQVIYTRATKG 321
KG I+ V H Q IYTRAT G
Sbjct: 87 KGTIRMVSAHSSQQIYTRATHG 108
>At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E)
ribosomal protein S25, Lycopersicon esculentum,
PIR2:S40089
Length = 108
Score = 68.9 bits (161), Expect = 1e-12
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +2
Query: 107 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELREK 259
+NN VLFD+ TY+KL E P++KLITP+++S+R+++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQATYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAK 87
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +1
Query: 256 KGLIKQVVQHHGQVIYTRAT 315
KG+I+ V H Q IYTRAT
Sbjct: 87 KGVIRMVAAHSSQQIYTRAT 106
>At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B)
Length = 108
Score = 68.5 bits (160), Expect = 1e-12
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +2
Query: 107 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELREK 259
+NN VLFD+ TY+KL E P++KLITP+++S+R+++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQGTYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAK 87
Score = 29.1 bits (62), Expect = 0.99
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +1
Query: 256 KGLIKQVVQHHGQVIYTRAT 315
KGLI+ V H Q IYTRAT
Sbjct: 87 KGLIRMVSAHSSQQIYTRAT 106
>At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A)
Length = 125
Score = 67.3 bits (157), Expect = 3e-12
Identities = 28/51 (54%), Positives = 42/51 (82%)
Frame = +2
Query: 107 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELREK 259
+NN VLFD+ TY+KL E P++KLITP+++S+RL++ GSLAR+A+ +L K
Sbjct: 53 VNNMVLFDQATYDKLMSEAPKFKLITPSILSDRLRINGSLARKAIRDLMVK 103
>At5g64950.1 68418.m08170 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 391
Score = 29.1 bits (62), Expect = 0.99
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -3
Query: 342 SLCDWIIALGRTRVDHLPMMLDYLFDETFSLSSMSALLAREPRTFNLSD 196
S C W++ + LP + YL S +++LL R+PR FNLS+
Sbjct: 166 SRCGWLLLSRDPNLFLLPN-ISYLETCGIVGSQLASLLRRQPRIFNLSE 213
>At1g14500.1 68414.m01719 ankyrin repeat family protein contains
Pfam domain, PF00023: Ankyrin repeat
Length = 436
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/49 (24%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = -3
Query: 171 YCGTSLYSFSYVGLSN--NTWLFNLSRTFPLDHFFFLALPPPDPSFFFC 31
+C LY+F + + + TW F + + + + +A+ P+P F C
Sbjct: 342 FCCALLYTFCLLPIGSLFTTWFFWIGASLGVSYALAMAIISPNPLLFLC 390
>At2g17250.1 68415.m01992 expressed protein weak similarity to
Ribosome biogenesis protein MAK21 (Swiss-Prot:Q12176)
[Saccharomyces cerevisiae]
Length = 577
Score = 27.9 bits (59), Expect = 2.3
Identities = 13/47 (27%), Positives = 29/47 (61%)
Frame = +2
Query: 110 NNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIEL 250
+++ + +KPT +K E L++PA +S+R+K++ + A + + L
Sbjct: 245 SDESISEKPTDKKKKTEKGDSTLLSPATISKRMKLKFTKAWISFLRL 291
>At5g55500.1 68418.m06912 beta-(1,2)-xylosyltransferase (XYLT)
identical to SP|Q9LDH0
Length = 534
Score = 27.1 bits (57), Expect = 4.0
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 366 FFFFNLYHSLCDWIIALGRTRVDHLP 289
F + NL+H++ DW A +RV LP
Sbjct: 244 FEYANLFHTVTDWYSAYVSSRVTGLP 269
>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
putative contains Pfam domain PF01851:
Proteasome/cyclosome repeat
Length = 1103
Score = 27.1 bits (57), Expect = 4.0
Identities = 23/69 (33%), Positives = 29/69 (42%), Gaps = 4/69 (5%)
Frame = -3
Query: 318 LGRTRVDHLPMMLD--YLFDETFSLSSMSALLAREP--RTFNLSDTTAGVISLYCGTSLY 151
LG LP+ L YL + S S + L RE T +S T I YC +L
Sbjct: 598 LGEALTRFLPLGLGLLYLGKQVGSQSYLIIFLVRESVEATAEVSKTFNEKIRKYCDMTLL 657
Query: 150 SFSYVGLSN 124
S +Y G N
Sbjct: 658 SCAYAGTGN 666
>At5g41380.1 68418.m05028 hypothetical protein
Length = 315
Score = 26.6 bits (56), Expect = 5.3
Identities = 18/43 (41%), Positives = 22/43 (51%)
Frame = -3
Query: 276 YLFDETFSLSSMSALLAREPRTFNLSDTTAGVISLYCGTSLYS 148
Y+FD + S SS S P F+ SDTT L+C LYS
Sbjct: 8 YIFDGSSS-SSSSVFRNSSPDMFS-SDTTT-TTDLFCNNELYS 47
>At5g27640.1 68418.m03311 eukaryotic translation initiation factor 3
subunit 9 / eIF-3 eta / eIF3b (TIF3B1) nearly identical
to SP|Q9C5Z1 Eukaryotic translation initiation factor 3
subunit 9 (eIF-3 eta) (eIF3 p110) (eIF3b) {Arabidopsis
thaliana}
Length = 712
Score = 26.6 bits (56), Expect = 5.3
Identities = 21/77 (27%), Positives = 31/77 (40%), Gaps = 2/77 (2%)
Frame = -3
Query: 264 ETFSLSSMSALLAREPRTFNLSDTTAGVISLYCGTSLYSFSYVGLSNNTWLFNLSRTFPL 85
E F++ + + E F +D Y T++ S + W FN + +
Sbjct: 537 EFFNVDELETMATAEH--FMATDIEWDPTGRYVATAVTSVHEMENGFTIWSFNGIMLYRI 594
Query: 84 --DHFFFLALPPPDPSF 40
DHFF LA P PSF
Sbjct: 595 LKDHFFQLAWRPRPPSF 611
>At2g23170.1 68415.m02768 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 595
Score = 26.6 bits (56), Expect = 5.3
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -2
Query: 145 LICGFIKQHLVVQLVTNFSFGPLLLLGFAAT 53
++CG + +H V++L F+ G L +GF T
Sbjct: 213 MLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243
>At1g24706.1 68414.m03104 expressed protein
Length = 1781
Score = 26.6 bits (56), Expect = 5.3
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 108 NLSRTFPLDHFFFLALPPPDP 46
+L ++ P DHF LPPP P
Sbjct: 1562 SLEKSHPDDHFHSQGLPPPPP 1582
>At5g54770.1 68418.m06822 thiazole biosynthetic enzyme, chloroplast
(ARA6) (THI1) (THI4) identical to SP|Q38814 Thiazole
biosynthetic enzyme, chloroplast precursor (ARA6)
{Arabidopsis thaliana}
Length = 349
Score = 26.2 bits (55), Expect = 7.0
Identities = 14/33 (42%), Positives = 22/33 (66%), Gaps = 3/33 (9%)
Frame = -3
Query: 273 LFDETFSLSSMSAL---LAREPRTFNLSDTTAG 184
LFD +F S++SA + +PR+F++ TTAG
Sbjct: 18 LFDSSFHGSAISAAPISIGLKPRSFSVRATTAG 50
>At1g48180.1 68414.m05378 expressed protein ; expression supported
by MPSS
Length = 239
Score = 26.2 bits (55), Expect = 7.0
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -3
Query: 204 LSDTTAGVISLYCGTSLYSFSYVGL 130
L + + +YCGTS SYVGL
Sbjct: 149 LQQDASAITGIYCGTSGEPASYVGL 173
>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
domain-containing protein contains Pfam domain PF00043:
Glutathione S-transferase, C-terminal domain
Length = 399
Score = 25.8 bits (54), Expect = 9.2
Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 3/44 (6%)
Frame = -2
Query: 259 LFPKLDECSSRQGTSDL*SFR---YDSRCD*LVLWDFLVQFLIC 137
L P LD+ + + ++ R YD RC +LWD + ++C
Sbjct: 161 LIPSLDKANRCRNLKEVYKSRSGGYDGRCTVPMLWDLRKKDVVC 204
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 25.8 bits (54), Expect = 9.2
Identities = 11/21 (52%), Positives = 14/21 (66%), Gaps = 1/21 (4%)
Frame = -3
Query: 192 TAGVISLY-CGTSLYSFSYVG 133
T G I LY CG + Y FS++G
Sbjct: 25 TPGKIGLYVCGITAYDFSHIG 45
>At4g36670.1 68417.m05203 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 493
Score = 25.8 bits (54), Expect = 9.2
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -3
Query: 183 VISLYCGTSLYSFSYVGLSNNTWLFNLSRTFPL 85
V+S+ S +F +GL TW+++ S FPL
Sbjct: 377 VLSIVAAYSFVAFFSIGLGPITWVYS-SEVFPL 408
>At2g02810.1 68415.m00226 UDP-galactose/UDP-glucose transporter
contains transmembrane domains; identical to
UDP-galactose/UDP-glucose transporter (GI:22651763)
[Arabidopsis thaliana] similar to UGTrel1 (GI:1669564)
[Rattus rattus]; identical to cDNA
UDP-galactose/UDP-glucose transporter GI:22651762
Length = 332
Score = 25.8 bits (54), Expect = 9.2
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -2
Query: 166 WDFLVQFLICGFIKQHLVVQLVTNF 92
WD L ++ ICG + Q+ + ++NF
Sbjct: 245 WDIL-KYCICGAVGQNFIFMTISNF 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,450,278
Number of Sequences: 28952
Number of extensions: 174748
Number of successful extensions: 541
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 541
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 497853200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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