Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00424
(789 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 153 4e-38
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 153 4e-38
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 153 4e-38
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 58 2e-09
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 52 1e-07
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 29 0.57
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 26 5.4
SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual 26 7.1
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 7.1
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.4
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 153 bits (370), Expect = 4e-38
Identities = 69/85 (81%), Positives = 76/85 (89%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFN
Sbjct: 250 DVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFN 309
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNVSVK++RRG V GDSKN+PP G
Sbjct: 310 VKNVSVKDIRRGNVCGDSKNDPPMG 334
Score = 144 bits (348), Expect = 2e-35
Identities = 63/96 (65%), Positives = 76/96 (79%)
Frame = +3
Query: 222 CW*LQKQPT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 401
C + P G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 402 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
E +PK +KSGDA I +VPSKP+CVE+F ++ PL
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPL 419
Score = 42.3 bits (95), Expect = 8e-05
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2
Query: 509 GRFAVRDMRQTVAVGVIKAV 568
GRFAVRDMRQTVAVGVIKAV
Sbjct: 420 GRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 153 bits (370), Expect = 4e-38
Identities = 69/85 (81%), Positives = 76/85 (89%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFN
Sbjct: 250 DVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFN 309
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNVSVK++RRG V GDSKN+PP G
Sbjct: 310 VKNVSVKDIRRGNVCGDSKNDPPMG 334
Score = 144 bits (348), Expect = 2e-35
Identities = 63/96 (65%), Positives = 76/96 (79%)
Frame = +3
Query: 222 CW*LQKQPT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 401
C + P G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 402 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
E +PK +KSGDA I +VPSKP+CVE+F ++ PL
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPL 419
Score = 42.3 bits (95), Expect = 8e-05
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2
Query: 509 GRFAVRDMRQTVAVGVIKAV 568
GRFAVRDMRQTVAVGVIKAV
Sbjct: 420 GRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 153 bits (370), Expect = 4e-38
Identities = 69/85 (81%), Positives = 76/85 (89%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFN
Sbjct: 250 DVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFN 309
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNVSVK++RRG V GDSKN+PP G
Sbjct: 310 VKNVSVKDIRRGNVCGDSKNDPPMG 334
Score = 144 bits (348), Expect = 2e-35
Identities = 63/96 (65%), Positives = 76/96 (79%)
Frame = +3
Query: 222 CW*LQKQPT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 401
C + P G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 402 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
E +PK +KSGDA I +VPSKP+CVE+F ++ PL
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPL 419
Score = 42.3 bits (95), Expect = 8e-05
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2
Query: 509 GRFAVRDMRQTVAVGVIKAV 568
GRFAVRDMRQTVAVGVIKAV
Sbjct: 420 GRFAVRDMRQTVAVGVIKAV 439
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 57.6 bits (133), Expect = 2e-09
Identities = 30/76 (39%), Positives = 45/76 (59%), Gaps = 2/76 (2%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVG 174
DV+ I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN G
Sbjct: 259 DVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCG 318
Query: 175 FNVKNVSVKELRRGYV 222
++++ ++L+RG +
Sbjct: 319 LLLRSIKREQLKRGMI 334
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 51.6 bits (118), Expect = 1e-07
Identities = 26/88 (29%), Positives = 44/88 (50%)
Frame = +3
Query: 237 KQPT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 416
K P F AQ+ +L P ++ GY+ V+ HTA FA++ K+D +T + ++
Sbjct: 551 KNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKP 609
Query: 417 PKSIKSGDAAIVNLVPSKPLCVESFQEF 500
P G I L P+C+E F+++
Sbjct: 610 PMFATKGMKIIAELETQTPVCMERFEDY 637
Score = 41.1 bits (92), Expect = 2e-04
Identities = 23/78 (29%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +1
Query: 10 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVK 186
K +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+
Sbjct: 476 KYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVR 535
Query: 187 NVSVKELRRGYVAGDSKN 240
+++ GYV +KN
Sbjct: 536 G-DDSDVQTGYVLTSTKN 552
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 29.5 bits (63), Expect = 0.57
Identities = 12/56 (21%), Positives = 32/56 (57%)
Frame = +1
Query: 97 NITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGLQI 264
++ ++ ++++ H + EA+ + +KN+ V+ GY + + +PP+G+Q+
Sbjct: 991 DVDIDLFNIKLEHTSEAEALDSHTAIYELKNILVQ----GYSQEEFRKSPPRGMQL 1042
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 207 QFLDGHVLYVETYIVSRYSFLESFV 133
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 26.2 bits (55), Expect = 5.4
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = -2
Query: 113 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIP 18
+SVV+ + +T V + + VST TGTV +P
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVP 117
>SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 25.8 bits (54), Expect = 7.1
Identities = 16/56 (28%), Positives = 26/56 (46%)
Frame = +1
Query: 112 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGLQILQLKS 279
V+ +E+H E + +PG+ N S+K + A + + KG I LKS
Sbjct: 289 VRDIELHSEKINWMLPGE----NTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKS 340
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 7.1
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -1
Query: 231 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 115
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 62 NTPVSTLPTGTVPIPPIL 9
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.4
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 448 MAASPDLMDFGLTSVDLPVRRSTFS 374
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,310,867
Number of Sequences: 5004
Number of extensions: 68933
Number of successful extensions: 219
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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