Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00424
(789 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 149 3e-36
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 149 3e-36
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 149 3e-36
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 149 3e-36
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 106 2e-23
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 55 5e-08
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 49 3e-06
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 48 7e-06
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 38 0.008
At4g27900.2 68417.m04005 expressed protein 32 0.50
At4g27900.1 68417.m04004 expressed protein 32 0.50
At1g79000.1 68414.m09212 p300/CBP acetyltransferase-related prot... 31 1.2
At4g29900.1 68417.m04254 calcium-transporting ATPase, plasma mem... 29 4.6
At1g27900.1 68414.m03419 RNA helicase, putative similar to SP|Q1... 29 4.6
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 6.1
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 149 bits (360), Expect = 3e-36
Identities = 66/85 (77%), Positives = 78/85 (91%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFN
Sbjct: 240 DVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFN 299
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNV+VK+L+RGYVA +SK++P KG
Sbjct: 300 VKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 133 bits (322), Expect = 1e-31
Identities = 56/89 (62%), Positives = 72/89 (80%)
Frame = +3
Query: 243 PT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 422
P GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK
Sbjct: 321 PAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPK 380
Query: 423 SIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
+K+GDA +V + P+KP+ VE+F E+PPL
Sbjct: 381 FLKNGDAGMVKMTPTKPMVVETFSEYPPL 409
Score = 42.7 bits (96), Expect = 3e-04
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2
Query: 500 PTPGRFAVRDMRQTVAVGVIKAVN 571
P GRFAVRDMRQTVAVGVIK+V+
Sbjct: 407 PPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 149 bits (360), Expect = 3e-36
Identities = 66/85 (77%), Positives = 78/85 (91%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFN
Sbjct: 240 DVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFN 299
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNV+VK+L+RGYVA +SK++P KG
Sbjct: 300 VKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 133 bits (322), Expect = 1e-31
Identities = 56/89 (62%), Positives = 72/89 (80%)
Frame = +3
Query: 243 PT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 422
P GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK
Sbjct: 321 PAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPK 380
Query: 423 SIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
+K+GDA +V + P+KP+ VE+F E+PPL
Sbjct: 381 FLKNGDAGMVKMTPTKPMVVETFSEYPPL 409
Score = 42.7 bits (96), Expect = 3e-04
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2
Query: 500 PTPGRFAVRDMRQTVAVGVIKAVN 571
P GRFAVRDMRQTVAVGVIK+V+
Sbjct: 407 PPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 149 bits (360), Expect = 3e-36
Identities = 66/85 (77%), Positives = 78/85 (91%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFN
Sbjct: 240 DVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFN 299
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNV+VK+L+RGYVA +SK++P KG
Sbjct: 300 VKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 133 bits (322), Expect = 1e-31
Identities = 56/89 (62%), Positives = 72/89 (80%)
Frame = +3
Query: 243 PT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 422
P GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK
Sbjct: 321 PAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPK 380
Query: 423 SIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
+K+GDA +V + P+KP+ VE+F E+PPL
Sbjct: 381 FLKNGDAGMVKMTPTKPMVVETFSEYPPL 409
Score = 42.7 bits (96), Expect = 3e-04
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2
Query: 500 PTPGRFAVRDMRQTVAVGVIKAVN 571
P GRFAVRDMRQTVAVGVIK+V+
Sbjct: 407 PPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 149 bits (360), Expect = 3e-36
Identities = 66/85 (77%), Positives = 78/85 (91%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 180
DVYKIGGIGTVPVGRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFN
Sbjct: 240 DVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFN 299
Query: 181 VKNVSVKELRRGYVAGDSKNNPPKG 255
VKNV+VK+L+RGYVA +SK++P KG
Sbjct: 300 VKNVAVKDLKRGYVASNSKDDPAKG 324
Score = 133 bits (322), Expect = 1e-31
Identities = 56/89 (62%), Positives = 72/89 (80%)
Frame = +3
Query: 243 PT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 422
P GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK
Sbjct: 321 PAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPK 380
Query: 423 SIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
+K+GDA +V + P+KP+ VE+F E+PPL
Sbjct: 381 FLKNGDAGMVKMTPTKPMVVETFSEYPPL 409
Score = 42.7 bits (96), Expect = 3e-04
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2
Query: 500 PTPGRFAVRDMRQTVAVGVIKAVN 571
P GRFAVRDMRQTVAVGVIK+V+
Sbjct: 407 PPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 106 bits (254), Expect = 2e-23
Identities = 44/75 (58%), Positives = 58/75 (77%)
Frame = +3
Query: 285 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 464
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTP 60
Query: 465 SKPLCVESFQEFPPL 509
+KP+ VE++ +PPL
Sbjct: 61 TKPMVVEAYSAYPPL 75
Score = 40.3 bits (90), Expect = 0.001
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +2
Query: 500 PTPGRFAVRDMRQTVAVGVIKAV 568
P GRFA+RDMRQTV VGVIK+V
Sbjct: 73 PPLGRFAIRDMRQTVGVGVIKSV 95
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 55.2 bits (127), Expect = 5e-08
Identities = 31/76 (40%), Positives = 41/76 (53%), Gaps = 2/76 (2%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTE--VKSVEMHHEALQEAVPGDNVG 174
DV+ I G GTV GRVE G +K G V T V VEM + L EA+ GDNVG
Sbjct: 294 DVFSITGRGTVATGRVERGTVKVGETVDLVGLRETRSYTVTGVEMFQKILDEALAGDNVG 353
Query: 175 FNVKNVSVKELRRGYV 222
++ + +++RG V
Sbjct: 354 LLLRGIQKADIQRGMV 369
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 49.2 bits (112), Expect = 3e-06
Identities = 24/91 (26%), Positives = 43/91 (47%), Gaps = 2/91 (2%)
Frame = +3
Query: 243 PT*GAADFTAQVIVLN--HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 416
P +F AQ+ +L + GY +L H C+ E+K ++D +T K +
Sbjct: 415 PVPAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDLKTRKPMKKK 474
Query: 417 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
+K+G A + + + +C+E F +FP L
Sbjct: 475 VLFVKNGAAVVCRIQVTNSICIEKFSDFPQL 505
Score = 42.7 bits (96), Expect = 3e-04
Identities = 20/80 (25%), Positives = 41/80 (51%)
Frame = +1
Query: 10 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKN 189
K +GTV +G+VE+G ++ G +V P +V ++ + ++ A PG+N+ +
Sbjct: 338 KFKDMGTVVMGKVESGSIREGDSLVVMPNKEQVKVVAIYCDEDKVKRAGPGENLRVRITG 397
Query: 190 VSVKELRRGYVAGDSKNNPP 249
+ +++ G+V N P
Sbjct: 398 IEDEDILSGFVLSSIVNPVP 417
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 48.0 bits (109), Expect = 7e-06
Identities = 28/79 (35%), Positives = 41/79 (51%), Gaps = 5/79 (6%)
Frame = +1
Query: 1 DVYKIGGIGTVPVGRVETGVLKPGTIVVF-----APANITTEVKSVEMHHEALQEAVPGD 165
DV+ I G GTV GR+E GV+K G V + + V VEM + L GD
Sbjct: 274 DVFSIQGRGTVATGRIEQGVIKVGEEVEILGLREGGVPLKSTVTGVEMFKKILDNGQAGD 333
Query: 166 NVGFNVKNVSVKELRRGYV 222
NVG ++ + ++++RG V
Sbjct: 334 NVGLLLRGLKREDIQRGMV 352
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 37.9 bits (84), Expect = 0.008
Identities = 17/61 (27%), Positives = 33/61 (54%)
Frame = +1
Query: 40 GRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGY 219
G++E G ++PG+ V+ P+ ++S+E +A A GDNV ++ + ++ G
Sbjct: 488 GKLEAGAVRPGSKVMVMPSGDQGTIRSLERDSQACTIARAGDNVALALQGIDANQVMAGD 547
Query: 220 V 222
V
Sbjct: 548 V 548
Score = 37.5 bits (83), Expect = 0.010
Identities = 21/81 (25%), Positives = 38/81 (46%)
Frame = +3
Query: 255 AADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKS 434
A V+VL I G H A A ++ +D +TG+ T+ +P+ + +
Sbjct: 559 ATHLELMVLVLEGATPILLGSQLEFHVHHAKEAATVVKLVAMLDPKTGQPTKKSPRCLTA 618
Query: 435 GDAAIVNLVPSKPLCVESFQE 497
+A++ + P+CVE+F E
Sbjct: 619 KQSAMLEVSLQNPVCVETFSE 639
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.50
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 417 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.50
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 417 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 509
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At1g79000.1 68414.m09212 p300/CBP acetyltransferase-related protein
2 (PCAT2) contains Pfam domains PF02135: TAZ zinc finger
and PF00569: Zinc finger, ZZ type; identical to cDNA
p300/CBP acetyltransferase-related protein 2
GI:12597460
Length = 1691
Score = 30.7 bits (66), Expect = 1.2
Identities = 20/60 (33%), Positives = 27/60 (45%), Gaps = 3/60 (5%)
Frame = +2
Query: 191 CPSRNCVVVMLLVTPKTTHLRGCRFYSSSHCA*PSWSNLKRL---HTSVGLPHCPHCLQI 361
CP RNCV V L H+ C ++ C+ P K L H S P+CP C+ +
Sbjct: 651 CPDRNCVTVQKL----WKHMDSC---AAPQCSYPRCLPTKTLINHHRSCKEPNCPVCIPV 703
>At4g29900.1 68417.m04254 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA10)
identical to SP|Q9SZR1 Potential calcium-transporting
ATPase 10, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 10) {Arabidopsis thaliana};
similar to SP|Q9LF79 Calcium-transporting ATPase 8,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
8) {Arabidopsis thaliana}
Length = 1069
Score = 28.7 bits (61), Expect = 4.6
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -2
Query: 158 GTASWRASWCISTDLTSVVMLAGAKTTMVPGFNT 57
G + WR W S DLT ++++ A ++ G T
Sbjct: 178 GRSFWRFVWEASQDLTLIILIVAAVASLALGIKT 211
>At1g27900.1 68414.m03419 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 700
Score = 28.7 bits (61), Expect = 4.6
Identities = 16/42 (38%), Positives = 25/42 (59%)
Frame = -2
Query: 179 LKPTLSPGTASWRASWCISTDLTSVVMLAGAKTTMVPGFNTP 54
L+P+LS + C+S LT V ML+ A+TT++P + P
Sbjct: 428 LEPSLSRTLIEANETGCLSQALTVVAMLS-AETTLLPARSKP 468
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 28.3 bits (60), Expect = 6.1
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +3
Query: 357 KFAEIKEKVDRRTGKSTEVNPKSI 428
+F + KEK+DR GK E+N K +
Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,553,636
Number of Sequences: 28952
Number of extensions: 373871
Number of successful extensions: 1166
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1098
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1163
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1775300800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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