Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00418
(639 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces... 106 2e-24
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 30 0.25
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 27 3.0
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 26 4.0
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 9.2
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 25 9.2
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 25 9.2
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 9.2
>SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 195
Score = 106 bits (255), Expect = 2e-24
Identities = 49/85 (57%), Positives = 64/85 (75%)
Frame = +2
Query: 254 KKFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRI 433
KKF+ +HV+F+ R+ILPKP K+RV QKRPRSRTLT+V++AILED+VFP EI+GKR
Sbjct: 85 KKFADRHVIFIAQRRILPKPGRKSRVT--QKRPRSRTLTAVHNAILEDIVFPTEIIGKRT 142
Query: 434 RVKLDGSQLIKVHLDKNQQTTIEHK 508
R DG + IKV LD T+++K
Sbjct: 143 RQATDGRKTIKVFLDNRDANTVDYK 167
Score = 71.3 bits (167), Expect = 1e-13
Identities = 36/80 (45%), Positives = 55/80 (68%), Gaps = 2/80 (2%)
Frame = +3
Query: 24 KIIKASGAEADSFETSISQALVELETNS-DLKAQLRELYITKAKEIELHN-KKSIIIYVP 197
KI+K S ++ + ++Q L +LE++S D+ +LR L IT A+E+E+ KK+I+++VP
Sbjct: 6 KIVKRSSSQPTETDLLVAQCLYDLESSSKDMAKELRPLQITSAREVEVGGGKKAIVVFVP 65
Query: 198 MPKLKAFQKFQIRLVRELER 257
P LKAF K Q RL RELE+
Sbjct: 66 QPLLKAFHKCQARLTRELEK 85
Score = 35.1 bits (77), Expect = 0.009
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = +1
Query: 508 MDTFQSVYKKLTGREVTFEFP 570
+ +F SVY KLTG+ VTFEFP
Sbjct: 168 LGSFSSVYHKLTGKNVTFEFP 188
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 30.3 bits (65), Expect = 0.25
Identities = 24/80 (30%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
Frame = +2
Query: 272 HVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRIRVKLDG 451
H V V D+ + P N+Q R +L+ + D + + V E V R K G
Sbjct: 1594 HTVLVLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFVVNGEYVEVR---KEAG 1650
Query: 452 SQLIKVHLD-KNQQTTIEHK 508
S ++ LD K+ Q EHK
Sbjct: 1651 SYILNPSLDLKHTQEMFEHK 1670
>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 472
Score = 26.6 bits (56), Expect = 3.0
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -1
Query: 516 GVHLCSIVVCWFLSKCTLMSC 454
GV+L SI++ FLS C L C
Sbjct: 138 GVYLYSILILLFLSHCVLRCC 158
>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 404
Score = 26.2 bits (55), Expect = 4.0
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +1
Query: 343 KEATLKDIDLCVRCYPRGLGLPC 411
++AT++++D C C RGL + C
Sbjct: 110 RKATIRNVDYCSACGGRGLFICC 132
>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 703
Score = 25.0 bits (52), Expect = 9.2
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = -3
Query: 361 P*AWPLLFVSNTSFVAGLRQDLTVSN 284
P A +LF+S TSF++G+ Q + ++N
Sbjct: 373 PPAAMILFISCTSFISGILQLVLLNN 398
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 9.2
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +2
Query: 305 PKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKR 430
P S + R N+++ RSR S + + LED+++ V R
Sbjct: 49 PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTR 90
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 25.0 bits (52), Expect = 9.2
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 492 VCWFLSKCTLMSCEPSNLTLMR 427
+C FL K T SC NL L++
Sbjct: 207 LCSFLPKSTYRSCRSDNLDLLK 228
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 25.0 bits (52), Expect = 9.2
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Frame = +3
Query: 123 LRELYITKAKEIELHNKKSIIIYVPMPKLKAFQKFQIR----LVRELERSSVVNM*SLLE 290
L +L + + I HN K + P P LK+ + R LV + + S++ SLL+
Sbjct: 1292 LEKLVFSMLRYIRFHNSKQSSKHSPSPTLKSVGPWAPRRVDVLVTSIGKDSILEKCSLLQ 1351
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,388,655
Number of Sequences: 5004
Number of extensions: 48034
Number of successful extensions: 138
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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