Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00409
(801 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g66610.1 68418.m08396 LIM domain-containing protein contains ... 46 4e-05
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 41 8e-04
At5g66620.1 68418.m08397 LIM domain-containing protein contains ... 38 0.008
At4g36860.1 68417.m05226 LIM domain-containing protein low simil... 37 0.018
At5g17890.1 68418.m02098 LIM domain-containing protein / disease... 36 0.041
At5g66630.1 68418.m08398 LIM domain-containing protein contains ... 33 0.17
At3g51340.1 68416.m05620 aspartyl protease family protein contai... 33 0.17
At5g24740.1 68418.m02920 expressed protein 31 1.2
At3g55770.1 68416.m06197 LIM domain-containing protein similar t... 29 3.6
At3g26240.1 68416.m03274 DC1 domain-containing protein contains ... 28 6.3
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 28 6.3
At2g39900.1 68415.m04904 LIM domain-containing protein similar t... 28 6.3
At2g24480.1 68415.m02925 zinc finger (C3HC4-type RING finger) fa... 28 6.3
At5g59930.1 68418.m07515 DC1 domain-containing protein / UV-B li... 28 8.3
At2g39830.1 68415.m04892 LIM domain-containing protein contains ... 28 8.3
At2g07630.1 68415.m00881 hypothetical protein 28 8.3
At1g08540.1 68414.m00946 RNA polymerase sigma subunit SigB (sigB... 28 8.3
At1g01780.1 68414.m00097 LIM domain-containing protein similar t... 28 8.3
>At5g66610.1 68418.m08396 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 529
Score = 45.6 bits (103), Expect = 4e-05
Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = +3
Query: 111 AVCNSCDKVIQ-GRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQH 242
++C+ C I+ GR V AL WHPE F C C KPI ++ +H
Sbjct: 199 SICDGCKSAIEYGRSVHALGVNWHPECFCCRYCDKPIAMHEYKEH 243
Score = 29.5 bits (63), Expect = 2.7
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = +1
Query: 511 ALDAKWHRDCFTCMKCRNPV 570
AL WH +CF C C P+
Sbjct: 216 ALGVNWHPECFCCRYCDKPI 235
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 41.1 bits (92), Expect = 8e-04
Identities = 18/46 (39%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = +3
Query: 114 VCNSCDKVI-QGRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNN 248
+C C+ I GR + LN WHPE F C C +PI +F N
Sbjct: 171 ICAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPISEYEFSTSGN 216
Score = 37.5 bits (83), Expect = 0.010
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +1
Query: 514 LDAKWHRDCFTCMKCRNPVTDSTFSVMENQPLCGKC 621
L++ WH +CF C C P+++ FS N P C
Sbjct: 188 LNSLWHPECFRCYGCSQPISEYEFSTSGNYPFHKAC 223
Score = 29.5 bits (63), Expect = 2.7
Identities = 15/66 (22%), Positives = 22/66 (33%), Gaps = 1/66 (1%)
Frame = +2
Query: 293 CHGCGDPITD-RVIQALGVSWHAHHFVXXXXXXXXXXXXFMEQAGRPYCSDCYADKFATR 469
C GC I R + L WH F F P+ CY +++ +
Sbjct: 172 CAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPISEYEFSTSGNYPFHKACYRERYHPK 231
Query: 470 CKGCGN 487
C C +
Sbjct: 232 CDVCSH 237
>At5g66620.1 68418.m08397 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 644
Score = 37.9 bits (84), Expect = 0.008
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = +3
Query: 108 PAVCNSCDKVIQ-GRIVTALNKKWHPEHFVCNTCRKPI 218
P++C C+ ++ G V L WHP F C C KPI
Sbjct: 283 PSLCGGCNFAVEHGGSVNILGVLWHPGCFCCRACHKPI 320
>At4g36860.1 68417.m05226 LIM domain-containing protein low
similarity to LIM-domain protein [Branchiostoma
floridae] GI:3360516, DRAL/Slim3/FHL2 [Homo sapiens]
GI:7209525; contains Pfam profile PF00412: LIM domain
Length = 351
Score = 36.7 bits (81), Expect = 0.018
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 526 WHRDCFTCMKCRNPVTDSTFSVMENQPLCGKC 621
WH +CF C C P+ D FS+ N+P C
Sbjct: 5 WHPECFCCNACDKPIIDYEFSMSGNRPYHKLC 36
Score = 34.7 bits (76), Expect = 0.072
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = +3
Query: 174 WHPEHFVCNTCRKPIDGAKFHQHNN 248
WHPE F CN C KPI +F N
Sbjct: 5 WHPECFCCNACDKPIIDYEFSMSGN 29
Score = 28.3 bits (60), Expect = 6.3
Identities = 13/50 (26%), Positives = 17/50 (34%)
Frame = +2
Query: 338 LGVSWHAHHFVXXXXXXXXXXXXFMEQAGRPYCSDCYADKFATRCKGCGN 487
+G WH F F RPY CY ++ +C C N
Sbjct: 1 MGGVWHPECFCCNACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHN 50
>At5g17890.1 68418.m02098 LIM domain-containing protein / disease
resistance protein-related low similarity to disease
resistance protein RPP4 [Arabidopsis thaliana]
GI:20270890; contains Pfam profiles PF00412: LIM domain,
PF00931: NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1613
Score = 35.5 bits (78), Expect = 0.041
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 117 CNSCDKVIQGRI-VTALNKKWHPEHFVCNTCRKPI 218
C C I+ I + A WHP+ F C CR+PI
Sbjct: 1240 CKDCKSAIEDGISINAYGSVWHPQCFCCLRCREPI 1274
Score = 32.3 bits (70), Expect = 0.39
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +1
Query: 511 ALDAKWHRDCFTCMKCRNPVTDSTFS 588
A + WH CF C++CR P+ + S
Sbjct: 1255 AYGSVWHPQCFCCLRCREPIAMNEIS 1280
>At5g66630.1 68418.m08398 LIM domain-containing protein contains low
similarity to Pfam profile PF00412: LIM domain
Length = 702
Score = 33.5 bits (73), Expect = 0.17
Identities = 14/45 (31%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = +3
Query: 111 AVCNSCDKVIQGR-IVTALNKKWHPEHFVCNTCRKPIDGAKFHQH 242
++C C+ ++ V L WHP F C +C KPI + H
Sbjct: 345 SMCGGCNSAVKHEESVNILGVLWHPGCFCCRSCDKPIAIHELENH 389
>At3g51340.1 68416.m05620 aspartyl protease family protein contains
Eukaryotic and viral aspartyl proteases active site,
PROSITE:PS00141
Length = 518
Score = 33.5 bits (73), Expect = 0.17
Identities = 23/76 (30%), Positives = 33/76 (43%), Gaps = 5/76 (6%)
Frame = -2
Query: 596 SITENVESVTGFLHFMHVKQSRCHLASSARWLYRRLD-----FRTPCSEWRTCLHSNRSN 432
SI N+ FL F+H + L + A W LD F PC+ TC+H +
Sbjct: 75 SIGSNLTLALNFLGFLHY--ANVSLGTPATWFLVALDTGSDLFWLPCNCGTTCIHDLKDA 132
Query: 431 RDGQPVP*TLHRQSPS 384
R + VP L+ + S
Sbjct: 133 RFSESVPLNLYTPNAS 148
>At5g24740.1 68418.m02920 expressed protein
Length = 3306
Score = 30.7 bits (66), Expect = 1.2
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -3
Query: 283 VMFGEAGHAMTPLLCWWNLAPSIGLRQVLQTKCSG 179
V F + G P+ WN+ IGL V+ T CSG
Sbjct: 3189 VDFRKQGFLGVPIDLVWNIEREIGLESVIHTDCSG 3223
>At3g55770.1 68416.m06197 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 199
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +1
Query: 526 WHRDCFTCMKCRNPVTDSTFSVMENQPLC 612
+H+ CF C C++ + S++S ME C
Sbjct: 30 YHKSCFKCTHCKSRLQLSSYSSMEGVLYC 58
>At3g26240.1 68416.m03274 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 922
Score = 28.3 bits (60), Expect = 6.3
Identities = 17/45 (37%), Positives = 23/45 (51%), Gaps = 5/45 (11%)
Frame = +1
Query: 505 HLALDAKWH--RDCFTCMKCRNPVTDSTFSVMENQ---PLCGKCA 624
HL L +K + R+C C K T TF+ +E + LC KCA
Sbjct: 731 HLFLTSKPNEERNCSVCAKAPGEYTKETFNCIEEECDYALCFKCA 775
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.3 bits (60), Expect = 6.3
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = +1
Query: 526 WHRDCFTCMKCRNPVTDSTFSVMENQPLC 612
+H+ CF C C+ + S +S M+ C
Sbjct: 30 YHKSCFRCTHCKGTLVISNYSSMDGVLYC 58
>At2g39900.1 68415.m04904 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 200
Score = 28.3 bits (60), Expect = 6.3
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +1
Query: 526 WHRDCFTCMKCRNPVTDSTFSVMENQPLC 612
+H+ CF C C++ + S +S ME C
Sbjct: 30 YHKACFKCSHCKSRLQLSNYSSMEGVVYC 58
>At2g24480.1 68415.m02925 zinc finger (C3HC4-type RING finger)
family protein low similarity to RING-H2 finger protein
RHY1a [Arabidopsis thaliana] GI:3790593; contains Pfam
profile PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 198
Score = 28.3 bits (60), Expect = 6.3
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +2
Query: 164 EQEMASRALCLQHLP*ANRWSQVPPAQQRCH--CVPCFTKHHSPRCHGCGDPI 316
E+E + A+CL+++ + + Q+P + H CV + H+ C C P+
Sbjct: 144 EEESETCAICLENMSRSENYCQMPYCKHCYHEGCVTKWVIGHNNSCPLCRKPV 196
>At5g59930.1 68418.m07515 DC1 domain-containing protein / UV-B
light-insensitive protein, putative similar to ULI3
(UV-B light insensitive) [Arabidopsis thaliana]
GI:17225050; contains Pfam profile PF03107: DC1 domain
Length = 656
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -1
Query: 417 CSMNPPPPISFLHPPQTKW*ACHDTPRAWITRSVMGSP 304
C MNPPPP + HP CHD P ++ + +P
Sbjct: 108 CGMNPPPP-AIEHP------ICHDHPLVFLKKRQEKTP 138
>At2g39830.1 68415.m04892 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 503
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/55 (25%), Positives = 25/55 (45%), Gaps = 1/55 (1%)
Frame = +3
Query: 114 VCNSCDKVI-QGRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVIACPASP 275
+C C+ I G + + +HPE F C++C I + ++ G+I P
Sbjct: 161 ICGGCNSDIGSGNYLGCMGTFFHPECFRCHSCGYAITEHEIPTNDAGLIEYRCHP 215
>At2g07630.1 68415.m00881 hypothetical protein
Length = 458
Score = 27.9 bits (59), Expect = 8.3
Identities = 13/43 (30%), Positives = 18/43 (41%)
Frame = +1
Query: 514 LDAKWHRDCFTCMKCRNPVTDSTFSVMENQPLCGKCA*FITNI 642
+D W C KC VT + ++ Q C C ITN+
Sbjct: 263 IDRDWSWYYIVCKKCNKKVTKVVMTSLKAQLWCETCRAPITNV 305
>At1g08540.1 68414.m00946 RNA polymerase sigma subunit SigB (sigB) /
sigma factor 2 (SIG2) identical to sigma factor SigB
[Arabidopsis thaliana] GI:5478446, plastid RNA
polymerase sigma-subunit [Arabidopsis thaliana]
GI:2879922, sigma factor [Arabidopsis thaliana]
GI:2597831, sigma factor 2 (SIG2) [Arabidopsis thaliana]
GI:2353173; contains Pfam profiles PF04545: Sigma-70,
region 4, PF04539: Sigma-70 region 3, PF04542: Sigma-70
region 2
Length = 572
Score = 27.9 bits (59), Expect = 8.3
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Frame = -3
Query: 475 LAASGELVCIAIGAIGTA---SLFHEPSTANLLLASTADE 365
+AA+ E V +A A+ A +LF + NLL +STAD+
Sbjct: 108 VAAAAEAVALARAAVKVAKDATLFKNSNNTNLLTSSTADK 147
>At1g01780.1 68414.m00097 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 205
Score = 27.9 bits (59), Expect = 8.3
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = +1
Query: 526 WHRDCFTCMKCRNPVTDSTFSVMENQPLC 612
+H+ CF C C+ + S +S M+ C
Sbjct: 30 YHKSCFRCTHCKGTLQMSNYSSMDGVLYC 58
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,279,806
Number of Sequences: 28952
Number of extensions: 422788
Number of successful extensions: 1560
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1329
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1558
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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