Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00404
(762 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65760.1 68418.m08275 serine carboxypeptidase S28 family prot... 85 4e-17
At2g24280.1 68415.m02901 serine carboxypeptidase S28 family prot... 65 4e-11
At5g22860.2 68418.m02673 serine carboxypeptidase S28 family prot... 61 7e-10
At5g22860.1 68418.m02672 serine carboxypeptidase S28 family prot... 61 7e-10
At3g28680.1 68416.m03579 prolylcarboxypeptidase-related weak sim... 52 4e-07
At2g18080.1 68415.m02102 serine carboxypeptidase S28 family prot... 45 5e-05
At4g36195.1 68417.m05150 serine carboxypeptidase S28 family prot... 42 3e-04
At4g36190.1 68417.m05149 serine carboxypeptidase S28 family prot... 33 0.16
At1g67640.1 68414.m07716 lysine and histidine specific transport... 32 0.36
At1g66235.1 68414.m07518 expressed protein ; expression supporte... 31 0.84
At2g34580.1 68415.m04248 hypothetical protein 30 1.9
At2g41060.1 68415.m05070 RNA recognition motif (RRM)-containing ... 28 5.9
At2g19380.1 68415.m02260 RNA recognition motif (RRM)-containing ... 28 5.9
At2g23300.1 68415.m02781 leucine-rich repeat transmembrane prote... 28 7.8
>At5g65760.1 68418.m08275 serine carboxypeptidase S28 family protein
similar to SP|P42785 Lysosomal Pro-X carboxypeptidase
precursor (EC 3.4.16.2) (Prolylcarboxypeptidase) (PRCP)
(Proline carboxypeptidase) {Homo sapiens}; contains Pfam
profile PF05577: Serine carboxypeptidase S28
Length = 515
Score = 85.4 bits (202), Expect = 4e-17
Identities = 38/83 (45%), Positives = 51/83 (61%), Gaps = 5/83 (6%)
Frame = +1
Query: 529 GWDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKYNVYPRENMARLEYGG---- 696
GW++QACTEMVMP+ S +N MF +N++ Y E+C+ + V PR E+GG
Sbjct: 365 GWNWQACTEMVMPMSSNQENSMFPGYGFNYSSYKEECWNTFRVNPRPKWVTTEFGGHDIA 424
Query: 697 -DLLQAASNIVFSYGLLDPWTAG 762
L SNI+FS GLLDPW+ G
Sbjct: 425 TTLKSFGSNIIFSNGLLDPWSGG 447
Score = 66.1 bits (154), Expect = 2e-11
Identities = 34/83 (40%), Positives = 48/83 (57%), Gaps = 1/83 (1%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPIHMFPGMTKCDLFNRIVTANFKR-NFYCSENIQKSW 179
GMLAA++R+KYPH+ GA+A+SAPI F + + F I + +FKR + C I+ SW
Sbjct: 195 GMLAAWMRLKYPHIAIGALASSAPILQFEDVVPPETFYDIASNDFKRESSSCFNTIKDSW 254
Query: 180 HLIRSYSSSVNGSEYLHKTLNLC 248
I + NG L KT + C
Sbjct: 255 DAIIAEGQKENGLLQLTKTFHFC 277
Score = 62.5 bits (145), Expect = 3e-10
Identities = 30/69 (43%), Positives = 45/69 (65%)
Frame = +2
Query: 257 NATDINSLIEYLESAYVTLAMVNYPFASSFLTPLPAQPVAVVCQYLNQNLANESLLEGIA 436
N+TD L ++L+SAY LAMV+YP+ + F+ PLP P+ VC+ ++ +N S+L+ I
Sbjct: 281 NSTD--DLSDWLDSAYSYLAMVDYPYPADFMMPLPGHPIREVCRKIDGAGSNASILDRIY 338
Query: 437 KAIDVYANY 463
I VY NY
Sbjct: 339 AGISVYYNY 347
>At2g24280.1 68415.m02901 serine carboxypeptidase S28 family protein
contains Pfam profile: PF05577 Serine carboxypeptidase
S28
Length = 494
Score = 65.3 bits (152), Expect = 4e-11
Identities = 32/83 (38%), Positives = 46/83 (55%), Gaps = 5/83 (6%)
Frame = +1
Query: 529 GWDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKYNVYPRENMARLEYGGDLLQ 708
GW +QACTEMVMP+ + Q+ M + + E C +Y V PR + E+GG ++
Sbjct: 352 GWQYQACTEMVMPMSCSNQS-MLPPYENDSEAFQEQCMTRYGVKPRPHWITTEFGGMRIE 410
Query: 709 A-----ASNIVFSYGLLDPWTAG 762
SNI+FS G+ DPW+ G
Sbjct: 411 TVLKRFGSNIIFSNGMQDPWSRG 433
Score = 63.7 bits (148), Expect = 1e-10
Identities = 31/83 (37%), Positives = 48/83 (57%), Gaps = 1/83 (1%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPIHMFPGMTKCDLFNRIVTANFK-RNFYCSENIQKSW 179
GMLAA+ R+KYPH+ GA+A+SAPI F + F ++ +FK + C + I++SW
Sbjct: 180 GMLAAWFRLKYPHITIGALASSAPILHFDNIVPLTSFYDAISQDFKDASINCFKVIKRSW 239
Query: 180 HLIRSYSSSVNGSEYLHKTLNLC 248
+ + S+ NG + L K C
Sbjct: 240 EELEAVSTMKNGLQELSKKFRTC 262
Score = 50.8 bits (116), Expect = 1e-06
Identities = 25/72 (34%), Positives = 40/72 (55%)
Frame = +2
Query: 284 EYLESAYVTLAMVNYPFASSFLTPLPAQPVAVVCQYLNQNLANESLLEGIAKAIDVYANY 463
++L A+V AMVNYP A++F+ PLP PV +C+ ++ S L+ A +Y NY
Sbjct: 273 DWLSGAFVYTAMVNYPTAANFMAPLPGYPVEQMCKIIDGFPRGSSNLDRAFAAASLYYNY 332
Query: 464 GKKTKQCVDYKE 499
K C + ++
Sbjct: 333 SGSEK-CFEMEQ 343
>At5g22860.2 68418.m02673 serine carboxypeptidase S28 family protein
contains Pfam profile: PF05577 serine carboxypeptidase
S28
Length = 439
Score = 61.3 bits (142), Expect = 7e-10
Identities = 31/82 (37%), Positives = 46/82 (56%), Gaps = 5/82 (6%)
Frame = +1
Query: 532 WDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKYNVYPRENMARLEYGGD---- 699
W +Q+C+E+VMPV Q+ MF +P+N T Y + C + V PR + +G
Sbjct: 357 WRWQSCSEIVMPVGYDKQDTMFPTAPFNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKL 416
Query: 700 -LLQAASNIVFSYGLLDPWTAG 762
L + SNI+FS GL DP++ G
Sbjct: 417 ILQKFGSNIIFSNGLSDPYSVG 438
Score = 56.0 bits (129), Expect = 3e-08
Identities = 32/83 (38%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPIHMFPGMTKCDLFNRIVTANFKR-NFYCSENIQKSW 179
GMLAA+ R+KYPH+ GA+A+SAP+ F + IVT FK + C I+ SW
Sbjct: 193 GMLAAWFRLKYPHIALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASERCYNTIRNSW 252
Query: 180 HLIRSYSSSVNGSEYLHKTLNLC 248
I + NG L K C
Sbjct: 253 IEIDRVAGKPNGLSILSKQFKTC 275
>At5g22860.1 68418.m02672 serine carboxypeptidase S28 family protein
contains Pfam profile: PF05577 serine carboxypeptidase
S28
Length = 502
Score = 61.3 bits (142), Expect = 7e-10
Identities = 31/82 (37%), Positives = 46/82 (56%), Gaps = 5/82 (6%)
Frame = +1
Query: 532 WDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKYNVYPRENMARLEYGGD---- 699
W +Q+C+E+VMPV Q+ MF +P+N T Y + C + V PR + +G
Sbjct: 357 WRWQSCSEIVMPVGYDKQDTMFPTAPFNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKL 416
Query: 700 -LLQAASNIVFSYGLLDPWTAG 762
L + SNI+FS GL DP++ G
Sbjct: 417 ILQKFGSNIIFSNGLSDPYSVG 438
Score = 56.0 bits (129), Expect = 3e-08
Identities = 32/83 (38%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPIHMFPGMTKCDLFNRIVTANFKR-NFYCSENIQKSW 179
GMLAA+ R+KYPH+ GA+A+SAP+ F + IVT FK + C I+ SW
Sbjct: 193 GMLAAWFRLKYPHIALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASERCYNTIRNSW 252
Query: 180 HLIRSYSSSVNGSEYLHKTLNLC 248
I + NG L K C
Sbjct: 253 IEIDRVAGKPNGLSILSKQFKTC 275
>At3g28680.1 68416.m03579 prolylcarboxypeptidase-related weak
similarity to SP|P42785| Lysosomal Pro-X
carboxypeptidase precursor (EC 3.4.16.2)
(Prolylcarboxypeptidase) (PRCP) (Proline
carboxypeptidase) {Homo sapiens}
Length = 199
Score = 52.0 bits (119), Expect = 4e-07
Identities = 29/82 (35%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +3
Query: 6 MLAAYIRIKYPHLVAGAIAASAPIHMFPGMTKCDLFNRIVTANFKR-NFYCSENIQKSWH 182
+LAA+ ++KYP++ GA+A+SAP+ F + IVT FK + C I KSW
Sbjct: 23 VLAAWFKLKYPYIALGALASSAPLLYFEDTLPKHGYFYIVTKVFKEMSKECHNKIHKSWD 82
Query: 183 LIRSYSSSVNGSEYLHKTLNLC 248
I ++ N L K LC
Sbjct: 83 EIDRIAAKPNSLSILSKNFKLC 104
>At2g18080.1 68415.m02102 serine carboxypeptidase S28 family protein
similar to SP|Q9NQE7 Thymus-specific serine protease
precursor (EC 3.4.-.-) {Homo sapiens}; contains Pfam
profile PF05577: Serine carboxypeptidase S28
Length = 365
Score = 45.2 bits (102), Expect = 5e-05
Identities = 28/76 (36%), Positives = 41/76 (53%), Gaps = 2/76 (2%)
Frame = +1
Query: 532 WDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKY--NVYPRENMARLEYGGDLL 705
W FQACTE+ + + D + N T + + C + +VYP+ + L YGGD L
Sbjct: 215 WWFQACTELGYFQVAP-KYDSVRSHQINTTFHLDLCKSLFGKDVYPKVDATNLYYGGDRL 273
Query: 706 QAASNIVFSYGLLDPW 753
AA+ I+F+ G DPW
Sbjct: 274 -AATKIIFTNGSEDPW 288
Score = 33.5 bits (73), Expect = 0.16
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPI 77
G L+A+ R+K+PHL G++A+SA +
Sbjct: 57 GALSAWFRLKFPHLTCGSLASSAVV 81
>At4g36195.1 68417.m05150 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 488
Score = 42.3 bits (95), Expect = 3e-04
Identities = 28/77 (36%), Positives = 37/77 (48%), Gaps = 3/77 (3%)
Frame = +1
Query: 532 WDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKY---NVYPRENMARLEYGGDL 702
W FQ CTE+ + ND + N T+Y D K VYP + L YG D
Sbjct: 338 WWFQVCTEVAYFQVAPA-NDSIRSHQIN-TEYHLDLCKSLFGKGVYPEVDATNLYYGSDR 395
Query: 703 LQAASNIVFSYGLLDPW 753
+ AA+ I+F+ G DPW
Sbjct: 396 I-AATKIIFTNGSQDPW 411
Score = 33.5 bits (73), Expect = 0.16
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPI 77
G L+A+ R+K+PHL G++A+SA +
Sbjct: 180 GALSAWFRLKFPHLTCGSLASSAVV 204
>At4g36190.1 68417.m05149 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 493
Score = 33.5 bits (73), Expect = 0.16
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = +3
Query: 3 GMLAAYIRIKYPHLVAGAIAASAPI 77
G L+A+ R+K+PHL G++A+SA +
Sbjct: 180 GALSAWFRLKFPHLTCGSLASSAVV 204
Score = 33.5 bits (73), Expect = 0.16
Identities = 27/87 (31%), Positives = 37/87 (42%), Gaps = 13/87 (14%)
Frame = +1
Query: 532 WDFQACTEMVMPVCSTGQNDMFEASPWNFTKYSEDCYKKY---NVYPRENMARLEYGGDL 702
W FQ CTE+ + ND + N T+Y D K VYP + L YG D
Sbjct: 338 WWFQVCTEVAYFQVAPA-NDSIRSHQIN-TEYHLDLCKSLFGKGVYPEVDATNLYYGSDK 395
Query: 703 LQAASN----------IVFSYGLLDPW 753
+ +S+ I+F+ G DPW
Sbjct: 396 IAVSSHLLILNFVATKIIFTNGSQDPW 422
>At1g67640.1 68414.m07716 lysine and histidine specific transporter,
putative similar to lysine and histidine specific
transporter GB:AAC49885 GI:2576361 (Arabidopsis
thaliana); contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 441
Score = 32.3 bits (70), Expect = 0.36
Identities = 15/42 (35%), Positives = 25/42 (59%)
Frame = -3
Query: 547 TPESPSRLRPNFGIVPFFIIYTLLGFLPIISVNVYSFGNSLE 422
TPE PS++ G+V +I+ + + P+ V Y FGNS++
Sbjct: 250 TPEKPSKIAMWKGVVVAYIVVAIC-YFPVAFVCYYIFGNSVD 290
>At1g66235.1 68414.m07518 expressed protein ; expression supported
by MPSS
Length = 265
Score = 31.1 bits (67), Expect = 0.84
Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 90 GMTKCDLFNRIVTANFK-RNFYCSENIQKSWHLIRSYSSSVNGSEYLHKTLNLCG 251
G + D+FN+ + ++F W++IR++ +G+ K LNLCG
Sbjct: 70 GASSDDIFNQAKEMLMQDKHFKSGWKFDHVWNIIRNFEKFKDGATQAKKVLNLCG 124
>At2g34580.1 68415.m04248 hypothetical protein
Length = 203
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/65 (24%), Positives = 37/65 (56%), Gaps = 1/65 (1%)
Frame = +2
Query: 86 SRNDEVRSLQ*DRNGQFQAEFLLLGKHTKIMALDKELLIISKRFRVSSQNAK-SMWGTNA 262
+RN + LQ ++ Q +L KH I ++++ L++ + ++++QN K ++ +N
Sbjct: 17 TRNQRLNLLQAEKELQVNKAQILASKHATIQSIERRCLMLEQ--KIAAQNLKITILRSNI 74
Query: 263 TDINS 277
D++S
Sbjct: 75 DDLDS 79
>At2g41060.1 68415.m05070 RNA recognition motif (RRM)-containing
protein similar to UBP1 interacting protein 1a
[Arabidopsis thaliana] GI:19574236; contains InterPro
entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM)
Length = 451
Score = 28.3 bits (60), Expect = 5.9
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 316 YGELSLCL*LSDAVTSAACGRGLSIFKSKFGKR 414
YGE+ C + D V+ + G G +FKS+ G R
Sbjct: 151 YGEIEDCKCVVDKVSGQSKGYGFILFKSRSGAR 183
>At2g19380.1 68415.m02260 RNA recognition motif (RRM)-containing
protein similar to UBP1 interacting protein 1a
[Arabidopsis thaliana] GI:19574236; contains InterPro
entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM); contains Pfam profile PF00096:
Zinc finger, C2H2 type
Length = 613
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +1
Query: 313 SYGELSLCL*LSDAVTSAACGRGLSIFKSKFGKR-IITRGNCQSYRRL 453
SYGE+ C + D T G G +FK++ G R + R + Y R+
Sbjct: 430 SYGEIEECSVVMDKDTGRGKGYGFVMFKTRKGAREALKRPEKRMYNRI 477
>At2g23300.1 68415.m02781 leucine-rich repeat transmembrane protein
kinase, putative
Length = 773
Score = 27.9 bits (59), Expect = 7.8
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Frame = -3
Query: 583 APSNKQALPSLCTPESPSRLRPN--FGIVPFFIIYTLLGFLPIISVNVYSFGNSL 425
+PS+ + C PES L+PN + + F +I L I+S++ GN L
Sbjct: 645 SPSSVGPISPYCAPESLRNLKPNPKWDVFGFGVILLELLTGKIVSIDEVGVGNGL 699
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,306,044
Number of Sequences: 28952
Number of extensions: 374107
Number of successful extensions: 962
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 952
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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