Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00382
(683 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 225 3e-59
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 220 8e-58
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 219 1e-57
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 47 1e-05
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 47 1e-05
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 46 3e-05
At2g22730.1 68415.m02694 transporter-related low similarity to s... 30 1.6
At4g36610.1 68417.m05196 hydrolase, alpha/beta fold family prote... 29 2.2
At5g60570.1 68418.m07594 kelch repeat-containing F-box family pr... 27 8.8
At4g33410.1 68417.m04748 signal peptide peptidase family protein... 27 8.8
At4g24390.2 68417.m03498 F-box family protein (FBX14) similar to... 27 8.8
At4g24390.1 68417.m03497 F-box family protein (FBX14) similar to... 27 8.8
At3g07100.1 68416.m00845 protein transport protein Sec24, putati... 27 8.8
At2g38630.1 68415.m04745 expressed protein 27 8.8
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 225 bits (549), Expect = 3e-59
Identities = 107/167 (64%), Positives = 132/167 (79%), Gaps = 1/167 (0%)
Frame = +1
Query: 7 QRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPY 186
QRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P+
Sbjct: 8 QRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVTFRHPF 67
Query: 187 KFKTRKELFIAPEALHRPICLL*-KKATLEVGNVMPVGAMPEGNIVCNLEEKMGDRGRLA 363
+FK +KELF+A E ++ L KKATL VGNV+P+ ++PEG +VCN+E +GDRG LA
Sbjct: 68 RFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRGVLA 127
Query: 364 RASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGR 504
RASG++A VI HNPD+ TR+KLPSG+KK++PS R M+G VAGGGR
Sbjct: 128 RASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGR 174
Score = 106 bits (254), Expect = 2e-23
Identities = 45/58 (77%), Positives = 49/58 (84%)
Frame = +3
Query: 510 KPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGKHQHIGKASTVKRGTSAGRK 683
KP+LKAG AYHKY+VKRN WP VRGVAMNPVEHPHGGG HQHIG ASTV+R G+K
Sbjct: 177 KPMLKAGNAYHKYRVKRNSWPKVRGVAMNPVEHPHGGGNHQHIGHASTVRRDAPPGQK 234
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 220 bits (537), Expect = 8e-58
Identities = 104/167 (62%), Positives = 130/167 (77%), Gaps = 1/167 (0%)
Frame = +1
Query: 7 QRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPY 186
QRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P+
Sbjct: 8 QRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVAFRHPF 67
Query: 187 KFKTRKELFIAPEALHRPICLL*-KKATLEVGNVMPVGAMPEGNIVCNLEEKMGDRGRLA 363
+FK +KELF+A E ++ L KKATL VGNV+P+ ++PEG ++CN+E +GDRG A
Sbjct: 68 RFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFA 127
Query: 364 RASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGR 504
RASG++A VI HNPD +R+KLPSG+KK++PS R M+G VAGGGR
Sbjct: 128 RASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGGGR 174
Score = 110 bits (265), Expect = 7e-25
Identities = 46/58 (79%), Positives = 50/58 (86%)
Frame = +3
Query: 510 KPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGKHQHIGKASTVKRGTSAGRK 683
KP+LKAG AYHKY+VKRNCWP VRGVAMNPVEHPHGGG HQHIG ASTV+R G+K
Sbjct: 177 KPMLKAGNAYHKYRVKRNCWPKVRGVAMNPVEHPHGGGNHQHIGHASTVRRDAPPGKK 234
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 219 bits (535), Expect = 1e-57
Identities = 107/175 (61%), Positives = 134/175 (76%), Gaps = 2/175 (1%)
Frame = +1
Query: 7 QRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDP 183
QRKGA GSVF SHT RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA V FR P
Sbjct: 8 QRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLARVAFRHP 67
Query: 184 YKFKTRKELFIAPEALHRPICLL*-KKATLEVGNVMPVGAMPEGNIVCNLEEKMGDRGRL 360
+++ +KELF+A E ++ L KKA L VGNV+P+G++PEG ++CN+E +GDRG L
Sbjct: 68 FRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDRGAL 127
Query: 361 ARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIANLF*K 525
ARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M+G VAGGGR F K
Sbjct: 128 ARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEKPFLK 182
Score = 112 bits (270), Expect = 2e-25
Identities = 48/58 (82%), Positives = 49/58 (84%)
Frame = +3
Query: 510 KPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGKHQHIGKASTVKRGTSAGRK 683
KP LKAG AYHKYK KRNCWP VRGVAMNPVEHPHGGG HQHIG ASTV+R SAG K
Sbjct: 178 KPFLKAGNAYHKYKAKRNCWPVVRGVAMNPVEHPHGGGNHQHIGHASTVRRDKSAGAK 235
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 46.8 bits (106), Expect = 1e-05
Identities = 24/73 (32%), Positives = 40/73 (54%)
Frame = +1
Query: 274 VGNVMPVGAMPEGNIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 453
+G+ MP+G M G I+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 454 LPSSNRGMVGIVA 492
+ + R +G V+
Sbjct: 118 INAKCRATIGTVS 130
Score = 41.1 bits (92), Expect = 7e-04
Identities = 18/31 (58%), Positives = 22/31 (70%), Gaps = 4/31 (12%)
Frame = +3
Query: 546 YKVKRNCW----PYVRGVAMNPVEHPHGGGK 626
YK ++ W P VRGVAMNP +HPHGGG+
Sbjct: 140 YKAGQSRWLGIRPKVRGVAMNPCDHPHGGGE 170
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 46.8 bits (106), Expect = 1e-05
Identities = 24/73 (32%), Positives = 40/73 (54%)
Frame = +1
Query: 274 VGNVMPVGAMPEGNIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 453
+G+ MP+G M G I+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 454 LPSSNRGMVGIVA 492
+ + R +G V+
Sbjct: 118 INAKCRATIGTVS 130
Score = 41.1 bits (92), Expect = 7e-04
Identities = 18/31 (58%), Positives = 22/31 (70%), Gaps = 4/31 (12%)
Frame = +3
Query: 546 YKVKRNCW----PYVRGVAMNPVEHPHGGGK 626
YK ++ W P VRGVAMNP +HPHGGG+
Sbjct: 140 YKAGQSRWLGIRPKVRGVAMNPCDHPHGGGE 170
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 45.6 bits (103), Expect = 3e-05
Identities = 20/27 (74%), Positives = 21/27 (77%), Gaps = 1/27 (3%)
Frame = +3
Query: 543 KYKVKRNCWPYVRGVAM-NPVEHPHGG 620
K +KRN W VRGVAM NPVEHPHGG
Sbjct: 411 KLMIKRNMWAKVRGVAMMNPVEHPHGG 437
>At2g22730.1 68415.m02694 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003974, GI:12003976, GI:12003972,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 510
Score = 29.9 bits (64), Expect = 1.6
Identities = 28/103 (27%), Positives = 39/103 (37%), Gaps = 2/103 (1%)
Frame = -1
Query: 557 FDLVLVVCP--SSFQNRFAIRPPPATIPTMPLLLDGRTFLAPDGSFTLVRLASGLCPITV 384
F V ++C + F + ATIP LL G TFL FT L S I +
Sbjct: 337 FGAVTIICGIVGTLSGGFILDRVTATIPNAFKLLSGATFLGAVFCFTAFTLKSLYGFIAL 396
Query: 383 AKFPEARARRPLSPIFSSRLHTMLPSGIAPTGITFPTSRVAFF 255
E +P+ LH + PS + P + T + F
Sbjct: 397 FALGELLVFATQAPVNYVCLHCVKPS-LRPLSMAISTVAIHIF 438
>At4g36610.1 68417.m05196 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas fluorescens] GI:1871461; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 317
Score = 29.5 bits (63), Expect = 2.2
Identities = 21/78 (26%), Positives = 32/78 (41%)
Frame = +1
Query: 244 CLL*KKATLEVGNVMPVGAMPEGNIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTR 423
CL+ L V +PVG G + + E D R SG+ T+ +A R
Sbjct: 116 CLVKGLRILGVDKFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNR 175
Query: 424 VKLPSGAKKVLPSSNRGM 477
+ S +LP+S G+
Sbjct: 176 LGFSSSTDLLLPTSVTGL 193
>At5g60570.1 68418.m07594 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 393
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = +1
Query: 184 YKFKTRKELFIAPEALHRPICLL*KKATLEVGNVMPV--GAMPEGNIVCNLEEKMGDRGR 357
+K+ R ++ E +HRP CL A+ +G + V G GNI+ + E GR
Sbjct: 161 WKYSLRSRCWVKCEGMHRPRCLF---ASGSLGGIAIVAGGTDMNGNILASAELYDSSSGR 217
>At4g33410.1 68417.m04748 signal peptide peptidase family protein
contains Pfam domain PF04258: Membrane protein of
unknown function (DUF435)
Length = 372
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2
Query: 547 YLWYALPAFKIGL 509
Y+WYALP + IGL
Sbjct: 301 YIWYALPGYAIGL 313
>At4g24390.2 68417.m03498 F-box family protein (FBX14) similar to
transport inhibitor response 1 protein GI:8777429 from
[Arabidopsis thaliana]
Length = 623
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/76 (23%), Positives = 34/76 (44%)
Frame = +1
Query: 85 YAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPICLL*KKA 264
+ E +++ + D + P L + R P+ K R F++ E LHR + +
Sbjct: 224 FPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLT 283
Query: 265 TLEVGNVMPVGAMPEG 312
+L G+ P +P+G
Sbjct: 284 SLGTGSFSP-DNVPQG 298
>At4g24390.1 68417.m03497 F-box family protein (FBX14) similar to
transport inhibitor response 1 protein GI:8777429 from
[Arabidopsis thaliana]
Length = 623
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/76 (23%), Positives = 34/76 (44%)
Frame = +1
Query: 85 YAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPICLL*KKA 264
+ E +++ + D + P L + R P+ K R F++ E LHR + +
Sbjct: 224 FPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLT 283
Query: 265 TLEVGNVMPVGAMPEG 312
+L G+ P +P+G
Sbjct: 284 SLGTGSFSP-DNVPQG 298
>At3g07100.1 68416.m00845 protein transport protein Sec24, putative
similar to protein transport protein Sec24A
(SEC24-related protein) [Homo sapiens] SWISS-PROT:O95486
Length = 1038
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/30 (30%), Positives = 14/30 (46%)
Frame = +3
Query: 204 GALHCSRSSTQANLFIVKESNS*SWKCDAC 293
G + C R T N F+ + W+C+ C
Sbjct: 367 GIIRCRRCRTYVNPFVTFTDSGRKWRCNIC 396
>At2g38630.1 68415.m04745 expressed protein
Length = 467
Score = 27.5 bits (58), Expect = 8.8
Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 2/60 (3%)
Frame = -3
Query: 279 SNFKSCFLSQ*TNWPV*S--FGSNEELLPCLELVWIAEVYNSQRCTSTRVMDYILNNSLD 106
+N K CFL+ + + S + N + L + V+ ++ Y+S +C STR+ +YIL D
Sbjct: 117 TNKKVCFLNVSPDEVIRSLFYNKNNDSLITVS-VYASDNYSSLKCRSTRI-EYILRGQAD 174
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,901,318
Number of Sequences: 28952
Number of extensions: 350356
Number of successful extensions: 1006
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 997
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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