Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00368
(632 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 161 4e-40
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 109 1e-24
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 109 1e-24
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 107 6e-24
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 79 2e-15
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 4e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 4e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 45 5e-05
At5g13650.2 68418.m01585 elongation factor family protein contai... 41 8e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 41 8e-04
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.84
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy... 29 3.4
At2g25730.1 68415.m03084 expressed protein 28 5.9
At1g73960.1 68414.m08565 expressed protein similar to TATA bindi... 28 5.9
At5g16420.1 68418.m01919 pentatricopeptide (PPR) repeat-containi... 27 7.8
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 7.8
At3g63180.1 68416.m07097 expressed protein 27 7.8
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 161 bits (391), Expect = 4e-40
Identities = 72/132 (54%), Positives = 97/132 (73%)
Frame = +2
Query: 218 KMLVCMSANCSARLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKA 397
+++ RL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q GTP++ +KA
Sbjct: 712 RVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKA 771
Query: 398 YLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPWRPCEPQSKPYNVVQETRKRKGLKE 577
YLPV ESFGF++ LR+ T GQAFPQCVFDHW+++ P EP ++ +V + RKRKGLKE
Sbjct: 772 YLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKGLKE 831
Query: 578 GLPDLTQYLDKL 613
+ L+++ DKL
Sbjct: 832 AMTPLSEFEDKL 843
Score = 134 bits (323), Expect = 6e-32
Identities = 59/84 (70%), Positives = 71/84 (84%)
Frame = +3
Query: 3 EGTAPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAI 182
E T PN++VD KGVQYLNEIKDSVVAGFQWA+KEG +AEEN+RG+ F + DV LH+DAI
Sbjct: 640 ETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAI 699
Query: 183 HRGGGQIIPTTRRCLYACLLTAQP 254
HRGGGQ+IPT RR +YA +TA+P
Sbjct: 700 HRGGGQVIPTARRVIYASQITAKP 723
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 109 bits (263), Expect = 1e-24
Identities = 62/120 (51%), Positives = 73/120 (60%), Gaps = 12/120 (10%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
RLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF
Sbjct: 840 RLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFET 899
Query: 434 DLRSNTGGQAFPQCVFDHWQVLP---------WRPCEP---QSKPYNVVQETRKRKGLKE 577
DLR +T GQAF VFDHW ++P RP EP Q + +TR+RKG+ E
Sbjct: 900 DLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959
Score = 78.2 bits (184), Expect = 4e-15
Identities = 36/84 (42%), Positives = 52/84 (61%), Gaps = 4/84 (4%)
Frame = +3
Query: 15 PNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAI 182
PNIL+D + + + +KDS+V GFQW A+EG + +E +R V+F I D + + +
Sbjct: 756 PNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPL 815
Query: 183 HRGGGQIIPTTRRCLYACLLTAQP 254
HRG GQ+IPT RR Y+ L A P
Sbjct: 816 HRGSGQMIPTARRVAYSAFLMATP 839
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 109 bits (263), Expect = 1e-24
Identities = 62/120 (51%), Positives = 73/120 (60%), Gaps = 12/120 (10%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
RLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF
Sbjct: 840 RLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFET 899
Query: 434 DLRSNTGGQAFPQCVFDHWQVLP---------WRPCEP---QSKPYNVVQETRKRKGLKE 577
DLR +T GQAF VFDHW ++P RP EP Q + +TR+RKG+ E
Sbjct: 900 DLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959
Score = 78.2 bits (184), Expect = 4e-15
Identities = 36/84 (42%), Positives = 52/84 (61%), Gaps = 4/84 (4%)
Frame = +3
Query: 15 PNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAI 182
PNIL+D + + + +KDS+V GFQW A+EG + +E +R V+F I D + + +
Sbjct: 756 PNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPL 815
Query: 183 HRGGGQIIPTTRRCLYACLLTAQP 254
HRG GQ+IPT RR Y+ L A P
Sbjct: 816 HRGSGQMIPTARRVAYSAFLMATP 839
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 107 bits (257), Expect = 6e-24
Identities = 61/120 (50%), Positives = 73/120 (60%), Gaps = 12/120 (10%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
RLMEPVY EIQ P V IY VL+RRRG+V + GTP +IVKA+LPV ESFGF
Sbjct: 826 RLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFET 885
Query: 434 DLRSNTGGQAFPQCVFDHWQVLP---------WRPCEP---QSKPYNVVQETRKRKGLKE 577
DLR +T GQAF VFDHW ++P RP EP Q + +TR+RKG+ E
Sbjct: 886 DLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 945
Score = 76.2 bits (179), Expect = 2e-14
Identities = 31/64 (48%), Positives = 43/64 (67%)
Frame = +3
Query: 63 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 242
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 762 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 821
Query: 243 TAQP 254
A P
Sbjct: 822 MATP 825
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 79.0 bits (186), Expect = 2e-15
Identities = 35/86 (40%), Positives = 51/86 (59%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
R++E +Y CE+ +G +Y VL+RRR + +E G+ +F V AY+PV+ESFGF
Sbjct: 873 RIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFAD 932
Query: 434 DLRSNTGGQAFPQCVFDHWQVLPWRP 511
+LR T G A V HW++L P
Sbjct: 933 ELRKGTSGGASALMVLSHWEMLEEDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 45.2 bits (102), Expect = 4e-05
Identities = 22/68 (32%), Positives = 38/68 (55%)
Frame = +2
Query: 257 LMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTAD 436
++EPV L E++ P G + G +N+R+G + Q ++ A +P+N FG++
Sbjct: 657 ILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTS 714
Query: 437 LRSNTGGQ 460
LRS T G+
Sbjct: 715 LRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 45.2 bits (102), Expect = 4e-05
Identities = 22/68 (32%), Positives = 38/68 (55%)
Frame = +2
Query: 257 LMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTAD 436
++EPV L E++ P G + G +N+R+G + Q ++ A +P+N FG++
Sbjct: 657 ILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTS 714
Query: 437 LRSNTGGQ 460
LRS T G+
Sbjct: 715 LRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 44.8 bits (101), Expect = 5e-05
Identities = 25/83 (30%), Positives = 42/83 (50%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
R++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F + +
Sbjct: 686 RMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQYVS 744
Query: 434 DLRSNTGGQAFPQCVFDHWQVLP 502
LR T G+A + V+P
Sbjct: 745 TLRGMTKGRASYTMQLAKFDVVP 767
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 40.7 bits (91), Expect = 8e-04
Identities = 21/84 (25%), Positives = 39/84 (46%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 434 DLRSNTGGQAFPQCVFDHWQVLPW 505
+ + + G A VFD + PW
Sbjct: 533 AILTASRGTAILNTVFDSYG--PW 554
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 40.7 bits (91), Expect = 8e-04
Identities = 21/84 (25%), Positives = 39/84 (46%)
Frame = +2
Query: 254 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 433
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 434 DLRSNTGGQAFPQCVFDHWQVLPW 505
+ + + G A VFD + PW
Sbjct: 532 AILTASRGTAILNTVFDSYG--PW 553
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 30.7 bits (66), Expect = 0.84
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +2
Query: 263 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 439
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 440 RSNTGGQA 463
+S T G A
Sbjct: 525 KSITSGYA 532
>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 455
Score = 28.7 bits (61), Expect = 3.4
Identities = 17/45 (37%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = +1
Query: 448 HRRTGLPAVRIRPLAGPPLET-VRTSEQALQRCTGNEKEERIEGR 579
HR TG+P I L GP + + V E A R G+EK + G+
Sbjct: 208 HRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGK 252
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 27.9 bits (59), Expect = 5.9
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = +1
Query: 1 PRVPPPTS---WSIAPKEFSTSMKLRTLLWLDSSGPLRKE 111
PR+ P + W + P + +T KL LLW S LR E
Sbjct: 423 PRLRPLVASMGWDLLPGKTATRRKLMRLLWTSDSQALRLE 462
>At1g73960.1 68414.m08565 expressed protein similar to TATA binding
protein associated factor (GI:2827282) [Homo sapiens];
similar to Transcription initiation factor TFIID 150 kDa
subunit (TAFII-150) (TAFII150) (Swiss-Prot:Q24325)
[Drosophila melanogaster]
Length = 1390
Score = 27.9 bits (59), Expect = 5.9
Identities = 26/84 (30%), Positives = 42/84 (50%), Gaps = 5/84 (5%)
Frame = +3
Query: 3 EGTAPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAI 182
E TA N+L++C K + L+E DSV + ++ A++N++ +R N Y V I
Sbjct: 117 EDTA-NLLINCCKPSKDLSEQLDSVT--LENGSQSSGEAKQNVKLIRIN-YWVEKIESGI 172
Query: 183 HRGGGQIIPTTR-----RCLYACL 239
H G I+ T RC + C+
Sbjct: 173 H-FDGNIVHTDNQMRRARCWFPCI 195
>At5g16420.1 68418.m01919 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 535
Score = 27.5 bits (58), Expect = 7.8
Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +2
Query: 488 WQVLPWRPCEPQSKPYNVVQETRKRKG-LKEGLPDLTQYLD 607
W + R C+P + YNV+ E + G +KEG+ L + L+
Sbjct: 423 WDDMYERKCKPNAFTYNVLIEGLSKNGNVKEGVRVLEEMLE 463
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 7.8
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +1
Query: 337 SWSRFRRVPGGRY-TYVHCEGL 399
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 7.8
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +3
Query: 396 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 500
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,634,681
Number of Sequences: 28952
Number of extensions: 351265
Number of successful extensions: 989
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 966
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 984
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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