Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00331
(663 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 138 3e-33
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 138 3e-33
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 138 3e-33
At1g29760.1 68414.m03639 expressed protein 31 0.68
At5g52440.1 68418.m06507 HCF106 protein identical to HCF106 [Ara... 29 2.8
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.8
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 28 4.8
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 4.8
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 4.8
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 4.8
At2g33460.1 68415.m04101 p21-rho-binding domain-containing prote... 28 4.8
At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 28 6.4
At1g50820.1 68414.m05715 hypothetical protein 28 6.4
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 138 bits (334), Expect = 3e-33
Identities = 63/84 (75%), Positives = 72/84 (85%)
Frame = +3
Query: 3 GVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYPA 182
G +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDGKVRTD TYPA
Sbjct: 25 GAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDGKVRTDKTYPA 84
Query: 183 GFMDVVSIEKTNELFRLIYDVKGR 254
GFMDVVSI KTNE FRL+YD KGR
Sbjct: 85 GFMDVVSIPKTNENFRLLYDTKGR 108
Score = 124 bits (300), Expect = 4e-29
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +2
Query: 248 GQITIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIAT 427
G+ +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK ND+I+LD+
Sbjct: 107 GRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEA 166
Query: 428 TKIMDFIKFESGNLCMITGGRNLGRVG 508
KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 167 NKIVEFIKFDVGNVVMVTGGRNRGRVG 193
Score = 61.3 bits (142), Expect = 6e-10
Identities = 26/41 (63%), Positives = 32/41 (78%)
Frame = +1
Query: 496 GACGPIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGK 618
G G I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGK 230
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 138 bits (334), Expect = 3e-33
Identities = 63/84 (75%), Positives = 72/84 (85%)
Frame = +3
Query: 3 GVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYPA 182
G +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDGKVRTD TYPA
Sbjct: 25 GAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDGKVRTDKTYPA 84
Query: 183 GFMDVVSIEKTNELFRLIYDVKGR 254
GFMDVVSI KTNE FRL+YD KGR
Sbjct: 85 GFMDVVSIPKTNENFRLLYDTKGR 108
Score = 124 bits (299), Expect = 5e-29
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +2
Query: 248 GQITIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIAT 427
G+ +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK ND+I+LD+
Sbjct: 107 GRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEE 166
Query: 428 TKIMDFIKFESGNLCMITGGRNLGRVG 508
KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 167 NKIVEFIKFDVGNVVMVTGGRNRGRVG 193
Score = 61.3 bits (142), Expect = 6e-10
Identities = 26/41 (63%), Positives = 32/41 (78%)
Frame = +1
Query: 496 GACGPIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGK 618
G G I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGK 230
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 138 bits (334), Expect = 3e-33
Identities = 63/84 (75%), Positives = 72/84 (85%)
Frame = +3
Query: 3 GVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYPA 182
G +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDGKVRTD TYPA
Sbjct: 25 GAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDGKVRTDKTYPA 84
Query: 183 GFMDVVSIEKTNELFRLIYDVKGR 254
GFMDVVSI KTNE FRL+YD KGR
Sbjct: 85 GFMDVVSIPKTNENFRLLYDTKGR 108
Score = 124 bits (299), Expect = 5e-29
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +2
Query: 248 GQITIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIAT 427
G+ +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK ND+I+LD+
Sbjct: 107 GRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEE 166
Query: 428 TKIMDFIKFESGNLCMITGGRNLGRVG 508
KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 167 NKIVEFIKFDVGNVVMVTGGRNRGRVG 193
Score = 61.3 bits (142), Expect = 6e-10
Identities = 26/41 (63%), Positives = 32/41 (78%)
Frame = +1
Query: 496 GACGPIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGK 618
G G I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGK 230
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.68
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3
Query: 166 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 74
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At5g52440.1 68418.m06507 HCF106 protein identical to HCF106
[Arabidopsis thaliana] GI:4894914; contains Pfam profile
PF02416: mttA/Hcf106 family
Length = 260
Score = 29.1 bits (62), Expect = 2.8
Identities = 21/94 (22%), Positives = 39/94 (41%), Gaps = 1/94 (1%)
Frame = +2
Query: 323 GPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGR-NLG 499
GPK + + + G+T+R P I+ + D +T + + + R N
Sbjct: 106 GPKGLAEVARNLGKTLRTFQPTIRELQDVSRDFKSTLEREIGLDDISTPNVYNQNRTNPV 165
Query: 500 RVGPSCPARDIPAPSTLCTSRTPRDTPSPRG*TT 601
+ P P +P+ T+ P D+ SP+ T+
Sbjct: 166 QPPPPPPPPSVPSTEAPVTANDPNDSQSPKAYTS 199
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 145 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 29
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = -2
Query: 647 ARPRDVRLRALPIMNTLFNLVAKVCPVESLMCTMSKEPGCLSRDTMGPH 501
A P++ + LPI+ L+ KVCP + L T G + PH
Sbjct: 263 ALPKNQKNYCLPIVLEFCKLLRKVCPDQKLYVTCRNTLGSMLETFDNPH 311
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 78 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 212
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 78 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 212
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 78 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 212
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At2g33460.1 68415.m04101 p21-rho-binding domain-containing protein
contains Pfam PF00786: P21-Rho-binding domain
Length = 224
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -1
Query: 639 TRCTPSCLADYEHVVQPRGEGVSRGVLDVHNVEGAGMSLAG 517
T TPS + D++ +G+ VSRG + +N +G AG
Sbjct: 47 TNTTPSWMNDFKTQEHEKGQVVSRGNSNKYNPQGTNQRGAG 87
>At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein
kinase, putative
Length = 658
Score = 27.9 bits (59), Expect = 6.4
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 353 HDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITG 484
H+ + +P ++N+ I+LDI++ I F NL +TG
Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167
>At1g50820.1 68414.m05715 hypothetical protein
Length = 528
Score = 27.9 bits (59), Expect = 6.4
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +3
Query: 240 DVKGRSQSTVSPLRRLSTSCVKSSVWRPDLRMFRTW*RMMAA 365
D + RS + L+ LSTS +K WR + F++W R MA+
Sbjct: 29 DCQNRSSALSVHLKALSTS-IKFWGWRFPNKKFKSWARKMAS 69
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,329,655
Number of Sequences: 28952
Number of extensions: 362186
Number of successful extensions: 1063
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1002
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1062
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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