BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00322
(292 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC56F8.15 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 2.2
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 25 2.2
SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces po... 25 3.0
SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos... 24 3.9
SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Ma... 24 5.2
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 23 6.9
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 23 9.1
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 23 9.1
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 23 9.1
SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|c... 23 9.1
>SPAC56F8.15 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 176
Score = 25.0 bits (52), Expect = 2.2
Identities = 19/54 (35%), Positives = 25/54 (46%)
Frame = -3
Query: 284 FFFNVDVQLVETLQIIFMLSLAQFYVLR*PCQIS*YFY*FQH*FTIHLNSYNLC 123
FFF +Q+ I+ LS +L PC I + F H FTI Y+LC
Sbjct: 27 FFF---LQMTPFYSILCFLSFFAL-LLHLPCSIYSHTLHFFHHFTIACYHYSLC 76
>SPAC144.09c |sfc2||RNA polymerase III transcription factor
TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
Length = 374
Score = 25.0 bits (52), Expect = 2.2
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = -3
Query: 146 HLNSYNLCHINSKSVTIHLDG 84
HL + CH N K + H DG
Sbjct: 69 HLKIHKRCHTNVKPFSCHYDG 89
>SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 432
Score = 24.6 bits (51), Expect = 3.0
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -2
Query: 258 SGNITNNLHVVTSAVLCFTVALSDFMIFLLIPTL 157
S N+T+NLH + C S ++ ++IP++
Sbjct: 184 SNNVTSNLHHIRIISKCSLKLKSMVLVSMIIPSI 217
>SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 24.2 bits (50), Expect = 3.9
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +1
Query: 22 WKTSHSAVNALNSSQIYK*NPPS 90
W+T S AL ++ +YK PP+
Sbjct: 255 WETYQSRKAALTANDVYKMQPPN 277
>SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 535
Score = 23.8 bits (49), Expect = 5.2
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2
Query: 228 VTSAVLCFTVALSDFMIFLLIPTLIH 151
VT ++CF +A+ D ++ L P L H
Sbjct: 111 VTQRIICFLLAI-DVLVVFLSPILRH 135
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 23.4 bits (48), Expect = 6.9
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -2
Query: 207 FTVALSDFMIFLLIPTLIHYTFE*LQFMSH 118
F+VA++ F+I L TL+ YT +F+ H
Sbjct: 182 FSVAITFFIIGLFTWTLVEYTMH--RFLFH 209
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 23.0 bits (47), Expect = 9.1
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +1
Query: 13 SP*WKTSHSAVNALNSSQIYK 75
+P +K SH V +NSS Y+
Sbjct: 1916 TPNFKPSHELVQLINSSPFYR 1936
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 23.0 bits (47), Expect = 9.1
Identities = 7/23 (30%), Positives = 11/23 (47%)
Frame = -3
Query: 95 HLDGGFYLYICDELSAFTAEWLV 27
H G+++Y C + WLV
Sbjct: 459 HFHWGYHIYACAVIGLLDPSWLV 481
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 23.0 bits (47), Expect = 9.1
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = +3
Query: 6 STFSVMENQPLCGKCA*FITNI-*IKSPIEMYSNTFRIDVT*IVTIQMYSELMLELIKIS 182
ST ++ +PLC C F+T IK+P + + I + T SEL+L++++ S
Sbjct: 603 STLTLHSLEPLCEFCVSFLTQANYIKNPY-LRAKLAEILYFGVQTHVGRSELLLDVVRTS 661
>SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 958
Score = 23.0 bits (47), Expect = 9.1
Identities = 11/43 (25%), Positives = 22/43 (51%)
Frame = -2
Query: 249 ITNNLHVVTSAVLCFTVALSDFMIFLLIPTLIHYTFE*LQFMS 121
IT + T + FT+ + DF++ ++I ++ F +Q S
Sbjct: 546 ITTKIEYFTICAIVFTMTVVDFVVGIVIGIIMACVFFVIQASS 588
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,025,661
Number of Sequences: 5004
Number of extensions: 15286
Number of successful extensions: 30
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30
length of database: 2,362,478
effective HSP length: 62
effective length of database: 2,052,230
effective search space used: 69775820
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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