BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00318 (714 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 52 6e-08 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 49 6e-07 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 42 1e-04 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 37 0.003 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.004 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 33 0.031 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 27 2.0 SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccha... 27 2.7 SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 3.5 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 26 4.7 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 26 4.7 SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subu... 25 8.1 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 25 8.1 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 52.4 bits (120), Expect = 6e-08 Identities = 20/48 (41%), Positives = 34/48 (70%) Frame = +2 Query: 275 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGXRESNGI 418 ++L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+G R+ + + Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDAL 119 Score = 48.4 bits (110), Expect = 1e-06 Identities = 21/51 (41%), Positives = 29/51 (56%) Frame = +3 Query: 96 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAA 248 + C +AE V + + +++ +V FYAPWCGHCK L PEY AA Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAA 65 Score = 47.6 bits (108), Expect = 2e-06 Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%) Frame = +3 Query: 111 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 236 ++ED++ L +F V+ + LV FYAPWCGHCK L P Y Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTY 395 Score = 31.9 bits (69), Expect = 0.094 Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 2/50 (4%) Frame = +2 Query: 260 TDDPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGXR 403 +DD V +AK+D TE S S+SG+PT+ F+ + + Y G R Sbjct: 403 SDDSNVVVAKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDR 448 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 49.2 bits (112), Expect = 6e-07 Identities = 22/68 (32%), Positives = 36/68 (52%) Frame = +3 Query: 33 KAPAKFEMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 212 + P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+ Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62 Query: 213 CKRLKPEY 236 CK+L P Y Sbjct: 63 CKKLVPTY 70 Score = 32.7 bits (71), Expect = 0.054 Identities = 17/44 (38%), Positives = 26/44 (59%), Gaps = 6/44 (13%) Frame = +2 Query: 290 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGXR 403 VDC + ++ C Q+ V G+PT+K+ KG S++YNG R Sbjct: 86 VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 1/40 (2%) Frame = +3 Query: 120 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 236 +V++L +F V+ LV FYA WCG+CKRL P Y Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180 Score = 39.1 bits (87), Expect = 6e-04 Identities = 14/20 (70%), Positives = 15/20 (75%) Frame = +3 Query: 177 ALVMFYAPWCGHCKRLKPEY 236 AL+ FYA WCGHCK L P Y Sbjct: 42 ALIEFYATWCGHCKSLAPVY 61 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 36.7 bits (81), Expect = 0.003 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = +3 Query: 141 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 236 S+F +++ Q +V F+A WCG CK + P++ Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 36.3 bits (80), Expect = 0.004 Identities = 14/29 (48%), Positives = 18/29 (62%) Frame = +3 Query: 144 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 230 D++ +S +V FYA WCG CK LKP Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP 55 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 33.5 bits (73), Expect = 0.031 Identities = 15/33 (45%), Positives = 21/33 (63%) Frame = +3 Query: 132 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 230 LTD+D + +S+ T + +Y P CG CKRL P Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62 Score = 27.1 bits (57), Expect = 2.7 Identities = 14/39 (35%), Positives = 22/39 (56%) Frame = +2 Query: 281 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNG 397 +A ++C K C+Q+S+ +PT +F K E EY G Sbjct: 333 VAHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVG 369 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 27.5 bits (58), Expect = 2.0 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +3 Query: 192 YAPWCGHCKRLKPEYAVAA 248 YA WCG CK + P ++ A Sbjct: 27 YADWCGPCKAISPLFSQLA 45 >SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 846 Score = 27.1 bits (57), Expect = 2.7 Identities = 18/56 (32%), Positives = 28/56 (50%) Frame = +3 Query: 432 RAQVGPSSKELLTVADFEAFTSKDEVWLSDSSKRNLT*KRVP*NS*QIKGGGYFCS 599 R +VG + +ELL V +SK V +SD +++ K + N +K G F S Sbjct: 51 RTKVGVAGRELLKVLAVNPNSSKPPVCISDLLQKSTQTKHISCN--DVKWGSSFAS 104 >SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizosaccharomyces pombe|chr 3|||Manual Length = 640 Score = 26.6 bits (56), Expect = 3.5 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +1 Query: 124 FSILQIPTFRLFYLNMIQPWSCF 192 F LQ+ FR + N+++PW CF Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/17 (58%), Positives = 14/17 (82%) Frame = +3 Query: 492 TSKDEVWLSDSSKRNLT 542 TS E+W S++S+RNLT Sbjct: 237 TSLAEIWKSEASRRNLT 253 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 26.2 bits (55), Expect = 4.7 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = -2 Query: 668 AWDDTRPHCFCIRFFSRTSLAEE*AKVTSSLNL 570 AW+ T H +CI SR++ ++ AK+ + L Sbjct: 37 AWNVTNGHVYCIHLCSRSANSKSIAKLDLCIEL 69 >SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subunit Apc13|Schizosaccharomyces pombe|chr 2|||Manual Length = 135 Score = 25.4 bits (53), Expect = 8.1 Identities = 10/18 (55%), Positives = 14/18 (77%) Frame = +1 Query: 580 EEVTFAHSSANEVLEKNR 633 +E+T HSSAN +L KN+ Sbjct: 45 DEMTIQHSSANLILMKNK 62 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 25.4 bits (53), Expect = 8.1 Identities = 8/15 (53%), Positives = 11/15 (73%) Frame = +3 Query: 180 LVMFYAPWCGHCKRL 224 L+ FYAPW CK++ Sbjct: 24 LLNFYAPWAAPCKQM 38 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,852,482 Number of Sequences: 5004 Number of extensions: 54566 Number of successful extensions: 164 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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