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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00318
         (714 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    52   6e-08
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    49   6e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    42   1e-04
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    37   0.003
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    36   0.004
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    33   0.031
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    27   2.0  
SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccha...    27   2.7  
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos...    27   3.5  
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch...    26   4.7  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    26   4.7  
SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subu...    25   8.1  
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    25   8.1  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 52.4 bits (120), Expect = 6e-08
 Identities = 20/48 (41%), Positives = 34/48 (70%)
 Frame = +2

Query: 275 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGXRESNGI 418
           ++L +VDCTE G   C ++S+ GYPTL +F+ G+  S+Y+G R+ + +
Sbjct: 73  ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDAL 119



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 21/51 (41%), Positives = 29/51 (56%)
 Frame = +3

Query: 96  YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAA 248
           + C +AE  V  +     + +++     +V FYAPWCGHCK L PEY  AA
Sbjct: 17  FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAA 65



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
 Frame = +3

Query: 111 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 236
           ++ED++ L   +F   V+ +    LV FYAPWCGHCK L P Y
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTY 395



 Score = 31.9 bits (69), Expect = 0.094
 Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
 Frame = +2

Query: 260 TDDPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGXR 403
           +DD  V +AK+D TE   S     S+SG+PT+  F+  +  +   Y G R
Sbjct: 403 SDDSNVVVAKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDR 448


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 22/68 (32%), Positives = 36/68 (52%)
 Frame = +3

Query: 33  KAPAKFEMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 212
           + P  F +F +  F L+ G+         + ++L   +F   +     +LV+FYAPWCG+
Sbjct: 4   RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62

Query: 213 CKRLKPEY 236
           CK+L P Y
Sbjct: 63  CKKLVPTY 70



 Score = 32.7 bits (71), Expect = 0.054
 Identities = 17/44 (38%), Positives = 26/44 (59%), Gaps = 6/44 (13%)
 Frame = +2

Query: 290 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGXR 403
           VDC  +  ++ C Q+ V G+PT+K+     KG    S++YNG R
Sbjct: 86  VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = +3

Query: 120 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 236
           +V++L   +F   V+      LV FYA WCG+CKRL P Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180



 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 14/20 (70%), Positives = 15/20 (75%)
 Frame = +3

Query: 177 ALVMFYAPWCGHCKRLKPEY 236
           AL+ FYA WCGHCK L P Y
Sbjct: 42  ALIEFYATWCGHCKSLAPVY 61


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = +3

Query: 141 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 236
           S+F +++ Q    +V F+A WCG CK + P++
Sbjct: 9   SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 36.3 bits (80), Expect = 0.004
 Identities = 14/29 (48%), Positives = 18/29 (62%)
 Frame = +3

Query: 144 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 230
           D++  +S     +V FYA WCG CK LKP
Sbjct: 27  DYNTRISADKVTVVDFYADWCGPCKYLKP 55


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 33.5 bits (73), Expect = 0.031
 Identities = 15/33 (45%), Positives = 21/33 (63%)
 Frame = +3

Query: 132 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 230
           LTD+D  + +S+  T  + +Y P CG CKRL P
Sbjct: 31  LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62



 Score = 27.1 bits (57), Expect = 2.7
 Identities = 14/39 (35%), Positives = 22/39 (56%)
 Frame = +2

Query: 281 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNG 397
           +A ++C    K  C+Q+S+  +PT  +F K E   EY G
Sbjct: 333 VAHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVG 369


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = +3

Query: 192 YAPWCGHCKRLKPEYAVAA 248
           YA WCG CK + P ++  A
Sbjct: 27  YADWCGPCKAISPLFSQLA 45


>SPAC4F8.11 |||WD repeat protein, human WDR24
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 846

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 18/56 (32%), Positives = 28/56 (50%)
 Frame = +3

Query: 432 RAQVGPSSKELLTVADFEAFTSKDEVWLSDSSKRNLT*KRVP*NS*QIKGGGYFCS 599
           R +VG + +ELL V      +SK  V +SD  +++   K +  N   +K G  F S
Sbjct: 51  RTKVGVAGRELLKVLAVNPNSSKPPVCISDLLQKSTQTKHISCN--DVKWGSSFAS 104


>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 640

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +1

Query: 124 FSILQIPTFRLFYLNMIQPWSCF 192
           F  LQ+  FR  + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229


>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
           Rev3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1480

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +3

Query: 492 TSKDEVWLSDSSKRNLT 542
           TS  E+W S++S+RNLT
Sbjct: 237 TSLAEIWKSEASRRNLT 253


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = -2

Query: 668 AWDDTRPHCFCIRFFSRTSLAEE*AKVTSSLNL 570
           AW+ T  H +CI   SR++ ++  AK+   + L
Sbjct: 37  AWNVTNGHVYCIHLCSRSANSKSIAKLDLCIEL 69


>SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subunit
           Apc13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 135

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 10/18 (55%), Positives = 14/18 (77%)
 Frame = +1

Query: 580 EEVTFAHSSANEVLEKNR 633
           +E+T  HSSAN +L KN+
Sbjct: 45  DEMTIQHSSANLILMKNK 62


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 8/15 (53%), Positives = 11/15 (73%)
 Frame = +3

Query: 180 LVMFYAPWCGHCKRL 224
           L+ FYAPW   CK++
Sbjct: 24  LLNFYAPWAAPCKQM 38


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,852,482
Number of Sequences: 5004
Number of extensions: 54566
Number of successful extensions: 164
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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