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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00315
         (678 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   124   9e-30
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...    70   4e-13
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...    69   8e-13
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    67   2e-12
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    47   3e-06
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    41   1e-04
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    39   8e-04
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    31   0.20 
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    27   2.5  
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce...    26   4.4  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    26   4.4  
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc...    26   5.8  
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    26   5.8  
SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosacch...    25   7.6  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  124 bits (300), Expect = 9e-30
 Identities = 55/60 (91%), Positives = 59/60 (98%)
 Frame = +1

Query: 76  DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 255
           +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61



 Score =  122 bits (294), Expect = 5e-29
 Identities = 52/56 (92%), Positives = 54/56 (96%)
 Frame = +3

Query: 252 QRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 419
           +RGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NR
Sbjct: 61  KRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNR 116



 Score = 64.1 bits (149), Expect = 2e-11
 Identities = 30/33 (90%), Positives = 31/33 (93%)
 Frame = +1

Query: 508 REKMTQIMFETFNTPAMYVAIQAVLSLYASGRT 606
           REKMTQI+FETFN PA YVAIQAVLSLYASGRT
Sbjct: 116 REKMTQIIFETFNAPAFYVAIQAVLSLYASGRT 148



 Score = 25.4 bits (53), Expect = 7.6
 Identities = 10/12 (83%), Positives = 10/12 (83%)
 Frame = +2

Query: 641 TRAIYEGYALPH 676
           T  IYEGYALPH
Sbjct: 162 TVPIYEGYALPH 173


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 30/53 (56%), Positives = 39/53 (73%)
 Frame = +1

Query: 94  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 252
           + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+
Sbjct: 12  ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQN 64



 Score = 64.9 bits (151), Expect = 1e-11
 Identities = 27/50 (54%), Positives = 37/50 (74%), Gaps = 1/50 (2%)
 Frame = +3

Query: 255 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPL 401
           RG+L ++ PIE GI+ NW DME+IW + + + +L   PEEHP+LLTE PL
Sbjct: 66  RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115



 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 16/33 (48%), Positives = 24/33 (72%)
 Frame = +1

Query: 508 REKMTQIMFETFNTPAMYVAIQAVLSLYASGRT 606
           +EK+ +  +ET N PA+  ++Q VL+LYAS RT
Sbjct: 121 KEKIAEYFYETLNVPALSFSLQPVLALYASART 153


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score = 68.5 bits (160), Expect = 8e-13
 Identities = 27/63 (42%), Positives = 45/63 (71%)
 Frame = +3

Query: 255 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR*VHRC 434
           R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P    +LLTE P+NP ANR    C
Sbjct: 62  RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANR-EKMC 120

Query: 435 RTM 443
            TM
Sbjct: 121 ETM 123



 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 29/59 (49%), Positives = 38/59 (64%), Gaps = 2/59 (3%)
 Frame = +1

Query: 82  EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQS 252
           E A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++
Sbjct: 2   ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60



 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 20/31 (64%), Positives = 23/31 (74%)
 Frame = +1

Query: 508 REKMTQIMFETFNTPAMYVAIQAVLSLYASG 600
           REKM + MFE +    +YVAIQAVLSLYA G
Sbjct: 116 REKMCETMFERYGFGGVYVAIQAVLSLYAQG 146


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 67.3 bits (157), Expect = 2e-12
 Identities = 27/51 (52%), Positives = 33/51 (64%)
 Frame = +3

Query: 267 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 419
           +L YPI HG + NWD ME+ W  + +  LR  PE+H  LLTE PLNP  NR
Sbjct: 89  SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENR 139



 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
 Frame = +1

Query: 94  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 243
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G  +  K SY+  +
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 17/30 (56%), Positives = 23/30 (76%)
 Frame = +1

Query: 508 REKMTQIMFETFNTPAMYVAIQAVLSLYAS 597
           RE   +IMFE+FN   +Y+A+QAVL+L AS
Sbjct: 139 RENTAEIMFESFNCAGLYIAVQAVLALAAS 168


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 46.8 bits (106), Expect = 3e-06
 Identities = 25/70 (35%), Positives = 35/70 (50%)
 Frame = +3

Query: 264 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR*VHRCRTM 443
           + +K  I +G V NWD    +W +    +L+  P EHP+L+TE   NP  NR V    TM
Sbjct: 63  MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENR-VKTLETM 121

Query: 444 HFASRPVGPY 473
             + R    Y
Sbjct: 122 FESLRCPATY 131



 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 16/34 (47%), Positives = 24/34 (70%)
 Frame = +1

Query: 79  EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 180
           +EV+A+V+D GS   + GF+G+D P+ V PS  G
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42



 Score = 29.9 bits (64), Expect = 0.35
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = +1

Query: 508 REKMTQIMFETFNTPAMYVAIQAVLSLYASGR 603
           R K  + MFE+   PA Y+A Q   + +ASG+
Sbjct: 114 RVKTLETMFESLRCPATYLAKQETCAAFASGK 145


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 17/43 (39%), Positives = 27/43 (62%)
 Frame = +3

Query: 279 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 407
           P E  +++NWD ME++  +TF  +L +   EHP+ +TE   NP
Sbjct: 86  PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANP 127



 Score = 35.9 bits (79), Expect = 0.005
 Identities = 15/32 (46%), Positives = 22/32 (68%)
 Frame = +1

Query: 94  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 189
           LV+DNGS   +AG+ G+  P+ VF ++V R R
Sbjct: 27  LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58



 Score = 28.7 bits (61), Expect = 0.82
 Identities = 10/28 (35%), Positives = 17/28 (60%)
 Frame = +1

Query: 508 REKMTQIMFETFNTPAMYVAIQAVLSLY 591
           R  MT+++FE +N P++   I  + S Y
Sbjct: 131 RSTMTELLFELYNAPSVAYGIDGLFSFY 158


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 20/57 (35%), Positives = 33/57 (57%)
 Frame = +1

Query: 79  EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 249
           EE+ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58



 Score = 34.3 bits (75), Expect = 0.016
 Identities = 13/44 (29%), Positives = 25/44 (56%)
 Frame = +3

Query: 288 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 419
           +GI+ +W+     W      +L+V P E+ +++TE   NP++ R
Sbjct: 74  NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVR 117


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 30.7 bits (66), Expect = 0.20
 Identities = 23/67 (34%), Positives = 28/67 (41%), Gaps = 5/67 (7%)
 Frame = -2

Query: 260 TSLLCASS----PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTT 96
           TS  C +S    PT  S  P+ PT+ P     P    N     S P         PL+TT
Sbjct: 389 TSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATT 448

Query: 95  NAATSSS 75
           N  TS+S
Sbjct: 449 NCTTSTS 455


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = +1

Query: 139 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 252
           G+  PRA F  ++  P H G+++ M  KD   G+E  S
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMS 537


>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 964

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 2/78 (2%)
 Frame = -2

Query: 440 SPASMNSPVGLGVEGSLSEQDGVFLGGDTQLIVE--GMMPNLLHVIPVSDDSVFDGVFEG 267
           SP S + PVG      L  Q G+ + G+T + V+  G MP L   +PVS     D     
Sbjct: 430 SPPSQHPPVGQIPSQYLPYQAGLKVPGNTPIPVKQVGGMP-LQSPLPVSMKPSADD--HS 486

Query: 266 QDTSLLCASSPTYESFCP 213
           + T      +PT  S+ P
Sbjct: 487 RATPTRSVEAPTLPSYAP 504


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
 Frame = -2

Query: 239 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 123
           S T +SF   P+PT  P    LPT   NT +    P+ PAL
Sbjct: 64  SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101


>SPCP1E11.04c |pal1||membrane associated protein Pal1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 425

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 10/18 (55%), Positives = 12/18 (66%)
 Frame = -3

Query: 133 NRPCTYRSHCLLPTRQLP 80
           NRP  YRSH  + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 7/21 (33%), Positives = 13/21 (61%)
 Frame = +3

Query: 279 PIEHGIVTNWDDMEKIWHHTF 341
           PI+ G V +W+ ++  W H +
Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133


>SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 297

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = +3

Query: 597 RSYTGIVLDSGDVSPHVPSTRDT 665
           R YTG+V  SGD+S  + S  D+
Sbjct: 117 REYTGLVTISGDISRGLKSAGDS 139


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,088,884
Number of Sequences: 5004
Number of extensions: 71092
Number of successful extensions: 238
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 235
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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