Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00310
(502 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35350.2 68417.m05022 cysteine endopeptidase, papain-type (XC... 34 0.047
At4g35350.1 68417.m05023 cysteine endopeptidase, papain-type (XC... 34 0.047
At1g29110.1 68414.m03563 cysteine proteinase, putative contains ... 34 0.047
At4g11320.1 68417.m01828 cysteine proteinase, putative contains ... 30 0.76
At2g34080.1 68415.m04172 cysteine proteinase, putative contains ... 30 0.76
At2g27420.1 68415.m03314 cysteine proteinase, putative contains ... 30 1.0
At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol pro... 29 1.3
At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP pr... 29 1.8
At5g11290.1 68418.m01318 expressed protein contains Pfam profile... 28 4.1
At1g29090.1 68414.m03561 peptidase C1A papain family protein con... 28 4.1
At4g30400.1 68417.m04318 zinc finger (C3HC4-type RING finger) fa... 27 5.4
At5g38790.1 68418.m04690 expressed protein predicted protein, Ar... 27 7.1
At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to calm... 27 7.1
At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to calm... 27 7.1
At4g31420.2 68417.m04461 zinc finger (C2H2 type) family protein ... 27 9.4
At4g31420.1 68417.m04460 zinc finger (C2H2 type) family protein ... 27 9.4
>At4g35350.2 68417.m05022 cysteine endopeptidase, papain-type (XCP1)
identical to papain-type cysteine endopeptidase XCP1
GI:6708181 from [Arabidopsis thaliana]
Length = 288
Score = 34.3 bits (75), Expect = 0.047
Identities = 15/45 (33%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = +3
Query: 123 PRHY-DLNQAKELFESFVKEHNREYKDDADRELHYQSFKKNLAEI 254
P H + ++ ELFES++ EH++ YK ++ ++ F++NL I
Sbjct: 38 PEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHI 82
Score = 29.9 bits (64), Expect = 1.0
Identities = 18/36 (50%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = +2
Query: 251 DNQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLP 355
D + NE N Y G+N+FAD T EE + R LGL P
Sbjct: 83 DQRNNEINSYW-LGLNEFADLTHEEFKGRYLGLAKP 117
>At4g35350.1 68417.m05023 cysteine endopeptidase, papain-type (XCP1)
identical to papain-type cysteine endopeptidase XCP1
GI:6708181 from [Arabidopsis thaliana]
Length = 355
Score = 34.3 bits (75), Expect = 0.047
Identities = 15/45 (33%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = +3
Query: 123 PRHY-DLNQAKELFESFVKEHNREYKDDADRELHYQSFKKNLAEI 254
P H + ++ ELFES++ EH++ YK ++ ++ F++NL I
Sbjct: 38 PEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHI 82
Score = 29.9 bits (64), Expect = 1.0
Identities = 18/36 (50%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = +2
Query: 251 DNQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLP 355
D + NE N Y G+N+FAD T EE + R LGL P
Sbjct: 83 DQRNNEINSYW-LGLNEFADLTHEEFKGRYLGLAKP 117
>At1g29110.1 68414.m03563 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 334
Score = 34.3 bits (75), Expect = 0.047
Identities = 20/70 (28%), Positives = 39/70 (55%), Gaps = 4/70 (5%)
Frame = +3
Query: 63 VSV-ALLIATVVMASSADTDPPR-HYDLNQAK--ELFESFVKEHNREYKDDADRELHYQS 230
VSV ++ +A +++ R H LN+ + + ++ + +R YKD++++E+ +
Sbjct: 2 VSVRSVFVALTILSMDLRISQARPHVTLNEQSIVDYHQQWMTQFSRVYKDESEKEMRLKV 61
Query: 231 FKKNLAEITN 260
FKKNL I N
Sbjct: 62 FKKNLKFIEN 71
>At4g11320.1 68417.m01828 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 371
Score = 30.3 bits (65), Expect = 0.76
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +3
Query: 144 QAKELFESFVKEHNREYKDDADRELHYQSFKKNLAEITN 260
+A +FES++ +H + Y A++E F+ NL ITN
Sbjct: 51 EATLMFESWMVKHGKVYDSVAEKERRLTIFEDNLRFITN 89
>At2g34080.1 68415.m04172 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 345
Score = 30.3 bits (65), Expect = 0.76
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 162 ESFVKEHNREYKDDADRELHYQSFKKNLAEITN 260
E ++ +REY+D+ ++ + FKKNL I N
Sbjct: 40 EQWMARFSREYRDELEKNMRRDVFKKNLKFIEN 72
>At2g27420.1 68415.m03314 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 348
Score = 29.9 bits (64), Expect = 1.0
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +3
Query: 147 AKELFESFVKEHNREYKDDADRELHYQSFKKNLAEITN 260
A E E ++ NR Y D+ ++ + FKKNL + N
Sbjct: 31 AIEKHEQWMARFNRVYSDETEKRNRFNIFKKNLEFVQN 68
>At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol
protease identical to SP|P43297 Cysteine proteinase
RD21A precursor (EC 3.4.22.-) {Arabidopsis thaliana},
thiol protease RD21A SP:P43297 from [Arabidopsis
thaliana]
Length = 462
Score = 29.5 bits (63), Expect = 1.3
Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = +2
Query: 227 IIQEKLGRDNQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLPAK 361
I ++ L ++ NEKN G+ +FAD T +E +S+ LG ++ K
Sbjct: 75 IFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 120
>At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP
protein (AALP) identical to AALP protein GI:7230640 from
[Arabidopsis thaliana]; similar to barley aleurain
Length = 358
Score = 29.1 bits (62), Expect = 1.8
Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +2
Query: 224 SIIQEKLGRDNQLNEKNPYTTFGINKFADYTPEE-QQSRLG 343
SI +E L N+K G+N+FAD T +E Q+++LG
Sbjct: 81 SIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRTKLG 121
>At5g11290.1 68418.m01318 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 353
Score = 27.9 bits (59), Expect = 4.1
Identities = 9/29 (31%), Positives = 18/29 (62%)
Frame = -2
Query: 294 MPNVVYGFFSFNWLSLPSFS*MIDNAALC 208
+ +++ F W+S+PSFS I ++ +C
Sbjct: 123 LTRIIHNHFKKFWMSIPSFSRSISDSKIC 151
>At1g29090.1 68414.m03561 peptidase C1A papain family protein
contains similarity to cysteine protease SPCP1
GI:13491750 from [Ipomoea batatas]; contains Pfam
profile PF00112: Papain family cysteine protease
Length = 355
Score = 27.9 bits (59), Expect = 4.1
Identities = 16/62 (25%), Positives = 33/62 (53%)
Frame = +3
Query: 60 FVSVALLIATVVMASSADTDPPRHYDLNQAKELFESFVKEHNREYKDDADRELHYQSFKK 239
F+ V+L I ++ + S T ++ A E + ++ +R Y D+ ++++ + FKK
Sbjct: 15 FMLVSLTILSMNLKVSQATSRVTFHEPIVA-EHHQQWMTRFSRVYSDELEKQMRFDVFKK 73
Query: 240 NL 245
NL
Sbjct: 74 NL 75
>At4g30400.1 68417.m04318 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 finger protein RHX1a
[Arabidopsis thaliana] GI:3790591; contains Pfam profile
PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 472
Score = 27.5 bits (58), Expect = 5.4
Identities = 18/50 (36%), Positives = 29/50 (58%)
Frame = -1
Query: 379 SENLCLLSRQPQSQPALLFLRSIISKFVNAKRSIWILLVQLVISAKFFLN 230
++N S PQ P L LRS + +N+K S ILL+ +++S FF++
Sbjct: 18 TQNFLSPSSLPQEPP--LSLRSSANFDLNSKISPSILLIIIILSIIFFIS 65
>At5g38790.1 68418.m04690 expressed protein predicted protein,
Arabidopsis thaliana; expression supported by MPSS
Length = 131
Score = 27.1 bits (57), Expect = 7.1
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Frame = +3
Query: 96 MASSADTDP--PRHYDLNQAKELFESFVKEHNREYKDDADRELHYQSFKK 239
MAS A + P R + N+A +L ++ E N + K+D D HY KK
Sbjct: 61 MASDASSGPNYQRFHQKNKALDL-KNGKNEGNSKSKNDDDHHNHYHDGKK 109
>At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to
calmodulin 4 [Arabidopsis thaliana] GI:16223, SP|P25854
Calmodulin-1/4 {Arabidopsis thaliana}
Length = 149
Score = 27.1 bits (57), Expect = 7.1
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +3
Query: 126 RHYDLNQAKELFESFVKEHNREYKDDADRELHYQSFKK 239
RH N ++L + V+E RE D D +++Y+ F K
Sbjct: 107 RHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVK 144
>At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to
calmodulin [Arabidopsis thaliana] GI:16223; nearly
identical to SP|P25854 Calmodulin-1/4 {Arabidopsis
thaliana}
Length = 149
Score = 27.1 bits (57), Expect = 7.1
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +3
Query: 126 RHYDLNQAKELFESFVKEHNREYKDDADRELHYQSFKK 239
RH N ++L + V+E RE D D +++Y+ F K
Sbjct: 107 RHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVK 144
>At4g31420.2 68417.m04461 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 405
Score = 26.6 bits (56), Expect = 9.4
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 183 NREYKDDADRELHYQS 230
N E+KD+ +R LHY+S
Sbjct: 10 NMEFKDEEERNLHYKS 25
>At4g31420.1 68417.m04460 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 404
Score = 26.6 bits (56), Expect = 9.4
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 183 NREYKDDADRELHYQS 230
N E+KD+ +R LHY+S
Sbjct: 10 NMEFKDEEERNLHYKS 25
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,573,425
Number of Sequences: 28952
Number of extensions: 177259
Number of successful extensions: 494
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 482
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 494
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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