BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00309X (425 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 125 3e-30 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 69 2e-13 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 67 9e-13 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 67 1e-12 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 46 2e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 41 7e-05 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 39 4e-04 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 32 0.043 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 28 0.70 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 1.6 SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 1.6 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 2.1 SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 26 2.8 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 2.8 SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 26 2.8 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 2.8 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 2.8 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 6.5 SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p... 22 8.3 SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual 24 8.6 SPAC7D4.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 8.6 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 125 bits (302), Expect = 3e-30 Identities = 54/57 (94%), Positives = 55/57 (96%) Frame = +3 Query: 255 SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 425 SKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NR Sbjct: 60 SKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNR 116 Score = 123 bits (297), Expect = 1e-29 Identities = 54/60 (90%), Positives = 59/60 (98%) Frame = +1 Query: 82 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQAK 261 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQ+K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 69.3 bits (162), Expect = 2e-13 Identities = 30/52 (57%), Positives = 38/52 (73%) Frame = +1 Query: 100 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 255 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQ 63 Score = 64.9 bits (151), Expect = 5e-12 Identities = 27/50 (54%), Positives = 37/50 (74%), Gaps = 1/50 (2%) Frame = +3 Query: 261 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPL 407 RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 67.3 bits (157), Expect = 9e-13 Identities = 27/51 (52%), Positives = 33/51 (64%) Frame = +3 Query: 273 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 425 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NR Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENR 139 Score = 42.7 bits (96), Expect = 2e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 100 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 249 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 66.9 bits (156), Expect = 1e-12 Identities = 24/55 (43%), Positives = 42/55 (76%) Frame = +3 Query: 261 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 425 R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANR Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANR 116 Score = 55.6 bits (128), Expect = 3e-09 Identities = 30/59 (50%), Positives = 38/59 (64%), Gaps = 2/59 (3%) Frame = +1 Query: 88 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQA 258 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA+A Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 46.4 bits (105), Expect = 2e-06 Identities = 20/52 (38%), Positives = 29/52 (55%) Frame = +3 Query: 270 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 425 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENR 114 Score = 40.3 bits (90), Expect = 1e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +1 Query: 85 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 186 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 41.1 bits (92), Expect = 7e-05 Identities = 17/43 (39%), Positives = 27/43 (62%) Frame = +3 Query: 285 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 413 P E +++NWD ME++ +TF +L + EHP+ +TE NP Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANP 127 Score = 35.9 bits (79), Expect = 0.003 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +1 Query: 100 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 195 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 38.7 bits (86), Expect = 4e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +1 Query: 85 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 255 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 Score = 34.3 bits (75), Expect = 0.008 Identities = 13/44 (29%), Positives = 25/44 (56%) Frame = +3 Query: 294 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 425 +GI+ +W+ W +L+V P E+ +++TE NP++ R Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVR 117 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 31.9 bits (69), Expect = 0.043 Identities = 23/67 (34%), Positives = 29/67 (43%), Gaps = 5/67 (7%) Frame = -1 Query: 266 TSFACASS----PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTT 102 TS +C +S PT S P+ PT+ P P N S P PL+TT Sbjct: 389 TSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATT 448 Query: 101 NAATSSS 81 N TS+S Sbjct: 449 NCTTSTS 455 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 27.9 bits (59), Expect = 0.70 Identities = 17/66 (25%), Positives = 29/66 (43%) Frame = -1 Query: 317 IPVSDDSVFDGVFEGQDTSFACASSPT*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 138 +P + +V +TSFA ++ S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 137 PALHIP 120 P L+ P Sbjct: 123 PQLNSP 128 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 1.6 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -1 Query: 245 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 129 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 127 Score = 26.6 bits (56), Expect = 1.6 Identities = 23/58 (39%), Positives = 27/58 (46%) Frame = -3 Query: 276 GSGYLFCLCLISYIRVLLSHTDHYALMAGPSHDRGEHGARSIISCETGLAHTGAIVYY 103 G G F CLI++IR +SH D EHG II C T L G I+YY Sbjct: 49 GLGIAFIYCLIAFIRE-MSH-------PSSRKDTMEHGL-PIILCST-LMLVGNILYY 96 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 26.2 bits (55), Expect = 2.1 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +1 Query: 145 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 249 G+ PRA F ++ P H G+++ M KD G+E Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534 >SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 25.8 bits (54), Expect = 2.8 Identities = 12/42 (28%), Positives = 21/42 (50%) Frame = -3 Query: 243 SYIRVLLSHTDHYALMAGPSHDRGEHGARSIISCETGLAHTG 118 +Y +LL D Y + G +DR + + + + C L H+G Sbjct: 226 TYPSLLLEEWDRYLMQNGSENDRPDSYSSTQLYCVLCLDHSG 267 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 2.8 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 127 TYRSHCLLPTRQLLRRTS*ISEI 59 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 25.8 bits (54), Expect = 2.8 Identities = 12/30 (40%), Positives = 14/30 (46%) Frame = -2 Query: 298 PCSMGYLRVRIPLLLVPHLLHKSPSVPYRP 209 P + Y PL VP +L PSV Y P Sbjct: 349 PLQLSYAHYATPLPAVPTVLSGLPSVAYTP 378 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.8 bits (54), Expect = 2.8 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = -2 Query: 253 VPHLLHKSPSVPYRPLRPDGGAFPRSRGTR 164 + L+ K+PS PY RP A S TR Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPTR 335 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 25.8 bits (54), Expect = 2.8 Identities = 7/21 (33%), Positives = 13/21 (61%) Frame = +3 Query: 285 PIEHGIVTNWDDMEKIWHHTF 347 PI+ G V +W+ ++ W H + Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 6.5 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +1 Query: 100 LVVDNGSGMCKAGFAG 147 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 >SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 21.8 bits (44), Expect(2) = 8.3 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = +3 Query: 267 ILTLKYPIEHGIVTN 311 I+T YP HGIV+N Sbjct: 125 IVTGLYPESHGIVSN 139 Score = 20.6 bits (41), Expect(2) = 8.3 Identities = 10/26 (38%), Positives = 12/26 (46%) Frame = +3 Query: 306 TNWDDMEKIWHHTFYNELRVAPEEHP 383 T WD E IW + N +R A P Sbjct: 162 TWWDKGEPIWVNAERNNVRSAVHMWP 187 >SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual Length = 426 Score = 24.2 bits (50), Expect = 8.6 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -1 Query: 422 VGLGVEGSLSEQDWVF 375 V + V GSL EQ+W F Sbjct: 96 VDMAVNGSLFEQEWAF 111 >SPAC7D4.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 551 Score = 24.2 bits (50), Expect = 8.6 Identities = 9/17 (52%), Positives = 12/17 (70%) Frame = +1 Query: 364 SPPRNTQSCSLRLPSTP 414 SPP++T+ S R PS P Sbjct: 285 SPPQSTRPSSTRFPSVP 301 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,934,650 Number of Sequences: 5004 Number of extensions: 42522 Number of successful extensions: 134 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 116 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 152416050 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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