Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00307
(703 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 124 4e-29
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 123 1e-28
At5g62520.1 68418.m07847 expressed protein 28 5.2
At3g15410.1 68416.m01955 leucine-rich repeat family protein cont... 28 5.2
At2g17930.1 68415.m02076 FAT domain-containing protein / phospha... 28 6.9
At1g30730.1 68414.m03756 FAD-binding domain-containing protein s... 28 6.9
At2g27980.1 68415.m03391 expressed protein 27 9.1
At1g30720.1 68414.m03755 FAD-binding domain-containing protein s... 27 9.1
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 124 bits (300), Expect = 4e-29
Identities = 57/92 (61%), Positives = 69/92 (75%)
Frame = +3
Query: 261 DAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVL 440
D + ++RK RL AE VQGRNVLC F GMD TTDKLR +VKKWQTLIEA++DVKTTD Y L
Sbjct: 76 DEDNAYRKIRLRAEDVQGRNVLCQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDSYTL 135
Query: 441 RVFCIGFTNKDSLSQRKTCYAQQLRSEQSERK 536
R+FCI FT + + ++TCYAQ + Q RK
Sbjct: 136 RLFCIAFTKRRANQVKRTCYAQSSQIRQIRRK 167
Score = 97.5 bits (232), Expect = 8e-21
Identities = 45/54 (83%), Positives = 50/54 (92%)
Frame = +1
Query: 91 VDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQ 252
VDPF++KDWYDVKAPS+F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ
Sbjct: 21 VDPFSKKDWYDVKAPSIFTHRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQ 74
Score = 52.8 bits (121), Expect = 2e-07
Identities = 21/48 (43%), Positives = 38/48 (79%)
Frame = +2
Query: 506 ATQVRAIRKKMCEIITRDVTNSELREVVNKLIPDSIAKDIEKACHGIY 649
++Q+R IR+KM +I+ R+ ++ +L+++V K IP++I ++IEKA GIY
Sbjct: 158 SSQIRQIRRKMRDIMVREASSCDLKDLVAKFIPEAIGREIEKATQGIY 205
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +1
Query: 649 PLRDVCIRKVKVLKRPRF 702
PL++V IRKVK+LK P+F
Sbjct: 206 PLQNVFIRKVKILKAPKF 223
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 123 bits (296), Expect = 1e-28
Identities = 57/92 (61%), Positives = 69/92 (75%)
Frame = +3
Query: 261 DAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVL 440
D + ++RK RL AE VQGRNVL F GMD TTDKLR +VKKWQTLIEA++DVKTTDGY L
Sbjct: 76 DEDNAYRKIRLRAEDVQGRNVLTQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDGYTL 135
Query: 441 RVFCIGFTNKDSLSQRKTCYAQQLRSEQSERK 536
R+FCI FT + + ++TCYAQ + Q RK
Sbjct: 136 RMFCIAFTKRRANQVKRTCYAQSSQIRQIRRK 167
Score = 95.5 bits (227), Expect = 3e-20
Identities = 44/54 (81%), Positives = 48/54 (88%)
Frame = +1
Query: 91 VDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQ 252
VDPF++KDWYDVKAP F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ
Sbjct: 21 VDPFSKKDWYDVKAPGSFTNRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQ 74
Score = 54.8 bits (126), Expect = 5e-08
Identities = 22/48 (45%), Positives = 38/48 (79%)
Frame = +2
Query: 506 ATQVRAIRKKMCEIITRDVTNSELREVVNKLIPDSIAKDIEKACHGIY 649
++Q+R IR+KM EI+ ++ ++ +L+E+V K IP++I ++IEKA GIY
Sbjct: 158 SSQIRQIRRKMSEIMVKEASSCDLKELVAKFIPEAIGREIEKATQGIY 205
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +1
Query: 649 PLRDVCIRKVKVLKRPRF 702
PL++V IRKVK+LK P+F
Sbjct: 206 PLQNVFIRKVKILKAPKF 223
>At5g62520.1 68418.m07847 expressed protein
Length = 309
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +2
Query: 503 PATQVRAIRKKMCEIITRDVTNSELREVVNKLIPDSIAKDIEKAC 637
P +Q+ I+K + R +T SEL + V + D + I KAC
Sbjct: 259 PPSQILVIQKHYKDQQNRRITRSELIQRVRSITGDKLLVHIIKAC 303
>At3g15410.1 68416.m01955 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Hcr2-5D
[Lycopersicon esculentum] gi|3894393|gb|AAC78596;
identical to leucine-rich repeat protein [Arabidopsis
thaliana] gi|2760084|emb|CAA76000
Length = 584
Score = 28.3 bits (60), Expect = 5.2
Identities = 16/34 (47%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +2
Query: 470 GLLEPTQDVLR-PATQVRAIRKKMCEIITRDVTN 568
GLLEPT +VLR +R+IR+ + E T+ V N
Sbjct: 542 GLLEPTLEVLRLDGNPLRSIRRPILERGTKAVLN 575
>At2g17930.1 68415.m02076 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF02259 FAT domain, PF00454
Phosphatidylinositol 3- and 4-kinase, PF02260: FATC
domain
Length = 3795
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = -2
Query: 222 SSLQSFRSNFRSLGTVDKRGADLPLAEHRRSLDIVPIFASEWVDNLLLN 76
S LQS RS F +L + K A + ++ + L++V + S+W+ LN
Sbjct: 1576 SWLQSNRSVFDTLVLIWKSPARISRLQNEQELNLVQVKESKWLVKCFLN 1624
>At1g30730.1 68414.m03756 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 526
Score = 27.9 bits (59), Expect = 6.9
Identities = 17/63 (26%), Positives = 30/63 (47%)
Frame = +2
Query: 413 CEDNRWIRSTCLLHWFHQ*GLLEPTQDVLRPATQVRAIRKKMCEIITRDVTNSELREVVN 592
C + RWI S +L W + PT +L P + K+ + + R V+ + L ++
Sbjct: 334 CTEMRWIDS--VLFWAGY-PVGTPTSVLLNPTVTKKLFMKRKSDYVKRPVSRTGLGLILK 390
Query: 593 KLI 601
KL+
Sbjct: 391 KLV 393
>At2g27980.1 68415.m03391 expressed protein
Length = 1037
Score = 27.5 bits (58), Expect = 9.1
Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +1
Query: 142 FSK-RQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQATLTRKGLSANSD 291
FSK +VG +++ T G + E +K V +V A +++ KGL +D
Sbjct: 76 FSKDEEVGEVVMSDTVGVGLEEEEVKSDVIDVDSALVESMSEDKGLEEKND 126
>At1g30720.1 68414.m03755 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 27.5 bits (58), Expect = 9.1
Identities = 16/63 (25%), Positives = 31/63 (49%)
Frame = +2
Query: 413 CEDNRWIRSTCLLHWFHQ*GLLEPTQDVLRPATQVRAIRKKMCEIITRDVTNSELREVVN 592
C + RWI S +L W + PT +L P + K+ + + R ++ ++L ++
Sbjct: 335 CTEMRWIDS--VLFWDDY-PVGTPTSVLLNPLVAKKLFMKRKSDYVKRLISRTDLGLILK 391
Query: 593 KLI 601
KL+
Sbjct: 392 KLV 394
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,772,600
Number of Sequences: 28952
Number of extensions: 336002
Number of successful extensions: 961
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 888
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 961
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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