BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00306 (685 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone este... 71 1e-14 AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. 71 1e-14 AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 50 3e-08 AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 50 3e-08 AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. 46 3e-07 AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. 44 2e-06 DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride... 23 3.6 DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride... 23 3.6 DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride... 23 3.6 DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride... 23 3.6 AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase... 23 3.6 DQ869053-1|ABJ09600.1| 459|Apis mellifera capa-like receptor pr... 22 6.3 >AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone esterase protein. Length = 567 Score = 70.5 bits (165), Expect = 1e-14 Identities = 38/89 (42%), Positives = 49/89 (55%), Gaps = 3/89 (3%) Frame = +2 Query: 257 ATRQCLPCPQRD--PYYP-DRFIGHEDCLCLNVFAPKMPGDERGCPVVFFVHGGNYKSGS 427 AT+ PC Q P P D+ G EDCL LNV+ P + PV+F++HGG ++ GS Sbjct: 78 ATKFGFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGGAFQFGS 137 Query: 428 ASAYGGQHLTQKDTILVTAQYRLGSLGTL 514 G ++L D I VT YRLG LG L Sbjct: 138 GIPMGAKYLMDSDVIFVTINYRLGILGFL 166 Score = 43.6 bits (98), Expect = 2e-06 Identities = 19/52 (36%), Positives = 30/52 (57%) Frame = +1 Query: 493 LRIFRNLSTDERDAAGNVGLFDLHAVMAWIQDYITLFGGDPTRVVVMGQGSG 648 L I LST++ GN+GL D + W+ + I FGG+P R+ ++G +G Sbjct: 160 LGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPKRITLIGLSAG 211 Score = 27.1 bits (57), Expect = 0.17 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 162 YYTFFGIRYAEPPLGPRRFQRPXR 233 Y + GI YA PP+G RF+ P + Sbjct: 45 YEAYEGIPYALPPVGKFRFKAPQK 68 >AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. Length = 567 Score = 70.5 bits (165), Expect = 1e-14 Identities = 38/89 (42%), Positives = 49/89 (55%), Gaps = 3/89 (3%) Frame = +2 Query: 257 ATRQCLPCPQRD--PYYP-DRFIGHEDCLCLNVFAPKMPGDERGCPVVFFVHGGNYKSGS 427 AT+ PC Q P P D+ G EDCL LNV+ P + PV+F++HGG ++ GS Sbjct: 78 ATKFGFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGGAFQFGS 137 Query: 428 ASAYGGQHLTQKDTILVTAQYRLGSLGTL 514 G ++L D I VT YRLG LG L Sbjct: 138 GIPMGAKYLMDSDVIFVTINYRLGILGFL 166 Score = 43.6 bits (98), Expect = 2e-06 Identities = 19/52 (36%), Positives = 30/52 (57%) Frame = +1 Query: 493 LRIFRNLSTDERDAAGNVGLFDLHAVMAWIQDYITLFGGDPTRVVVMGQGSG 648 L I LST++ GN+GL D + W+ + I FGG+P R+ ++G +G Sbjct: 160 LGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPKRITLIGLSAG 211 Score = 27.1 bits (57), Expect = 0.17 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 162 YYTFFGIRYAEPPLGPRRFQRPXR 233 Y + GI YA PP+G RF+ P + Sbjct: 45 YEAYEGIPYALPPVGKFRFKAPQK 68 >AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase protein. Length = 492 Score = 49.6 bits (113), Expect = 3e-08 Identities = 21/62 (33%), Positives = 33/62 (53%) Frame = +1 Query: 481 GAVSLRIFRNLSTDERDAAGNVGLFDLHAVMAWIQDYITLFGGDPTRVVVMGQGSGWSAA 660 GA T+ +A GN+GL+D + W++D FGGDP + + G+ +G S+ Sbjct: 200 GAFGFLYLNKHFTNSEEAPGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSV 259 Query: 661 SL 666 SL Sbjct: 260 SL 261 Score = 37.9 bits (84), Expect = 9e-05 Identities = 28/100 (28%), Positives = 49/100 (49%), Gaps = 18/100 (18%) Frame = +2 Query: 281 PQRDPYYPDRFIGHEDCLCLNVFAPK----------MPGDERG-----CPVVFFVHGGNY 415 P + + P+ I EDCL LN++ P+ PG G P++ +++GG + Sbjct: 111 PGEEMWNPNTNIS-EDCLYLNIWVPQKYRLRHKGDGSPGGNGGPRNGLLPLLVWIYGGGF 169 Query: 416 KSGSAS--AYGGQHLTQKDTILVTA-QYRLGSLGTLVLMK 526 SG+A+ Y + +++ + QYR+G+ G L L K Sbjct: 170 MSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNK 209 Score = 34.3 bits (75), Expect = 0.001 Identities = 11/21 (52%), Positives = 18/21 (85%) Frame = +3 Query: 165 YTFFGIRYAEPPLGPRRFQRP 227 + F+GI +A+PP+GP RF++P Sbjct: 60 HVFYGIPFAKPPIGPLRFRKP 80 >AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase protein. Length = 628 Score = 49.6 bits (113), Expect = 3e-08 Identities = 21/62 (33%), Positives = 33/62 (53%) Frame = +1 Query: 481 GAVSLRIFRNLSTDERDAAGNVGLFDLHAVMAWIQDYITLFGGDPTRVVVMGQGSGWSAA 660 GA T+ +A GN+GL+D + W++D FGGDP + + G+ +G S+ Sbjct: 200 GAFGFLYLNKHFTNSEEAPGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSV 259 Query: 661 SL 666 SL Sbjct: 260 SL 261 Score = 37.9 bits (84), Expect = 9e-05 Identities = 28/100 (28%), Positives = 49/100 (49%), Gaps = 18/100 (18%) Frame = +2 Query: 281 PQRDPYYPDRFIGHEDCLCLNVFAPK----------MPGDERG-----CPVVFFVHGGNY 415 P + + P+ I EDCL LN++ P+ PG G P++ +++GG + Sbjct: 111 PGEEMWNPNTNIS-EDCLYLNIWVPQKYRLRHKGDGSPGGNGGPRNGLLPLLVWIYGGGF 169 Query: 416 KSGSAS--AYGGQHLTQKDTILVTA-QYRLGSLGTLVLMK 526 SG+A+ Y + +++ + QYR+G+ G L L K Sbjct: 170 MSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNK 209 Score = 34.3 bits (75), Expect = 0.001 Identities = 11/21 (52%), Positives = 18/21 (85%) Frame = +3 Query: 165 YTFFGIRYAEPPLGPRRFQRP 227 + F+GI +A+PP+GP RF++P Sbjct: 60 HVFYGIPFAKPPIGPLRFRKP 80 >AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. Length = 62 Score = 46.0 bits (104), Expect = 3e-07 Identities = 23/64 (35%), Positives = 41/64 (64%), Gaps = 2/64 (3%) Frame = +2 Query: 323 EDCLCLNVFAPKMPGDERGCPVVFFVHGGNYKSGSASAY--GGQHLTQKDTILVTAQYRL 496 EDCL L+V+ + ++ PV+F+VH G + SG++S + +L KD ++V++ YR+ Sbjct: 2 EDCLYLDVYTNSL---DQSKPVMFYVHEGAFISGTSSFHEMRPDYLLPKDVVVVSSNYRV 58 Query: 497 GSLG 508 G+ G Sbjct: 59 GAFG 62 >AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. Length = 169 Score = 43.6 bits (98), Expect = 2e-06 Identities = 19/52 (36%), Positives = 30/52 (57%) Frame = +1 Query: 493 LRIFRNLSTDERDAAGNVGLFDLHAVMAWIQDYITLFGGDPTRVVVMGQGSG 648 L I LST++ GN+GL D + W+ + I FGG+P R+ ++G +G Sbjct: 31 LGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPKRITLIGLSAG 82 >DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride channel variant 4 protein. Length = 489 Score = 22.6 bits (46), Expect = 3.6 Identities = 7/13 (53%), Positives = 11/13 (84%) Frame = +3 Query: 267 NAYRVRNETLTIP 305 NAY ++N+T+T P Sbjct: 235 NAYLIKNQTITCP 247 >DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride channel variant 3 protein. Length = 475 Score = 22.6 bits (46), Expect = 3.6 Identities = 7/13 (53%), Positives = 11/13 (84%) Frame = +3 Query: 267 NAYRVRNETLTIP 305 NAY ++N+T+T P Sbjct: 235 NAYLIKNQTITCP 247 >DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride channel variant 1 protein. Length = 509 Score = 22.6 bits (46), Expect = 3.6 Identities = 7/13 (53%), Positives = 11/13 (84%) Frame = +3 Query: 267 NAYRVRNETLTIP 305 NAY ++N+T+T P Sbjct: 286 NAYLIKNQTITCP 298 >DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride channel protein. Length = 458 Score = 22.6 bits (46), Expect = 3.6 Identities = 7/13 (53%), Positives = 11/13 (84%) Frame = +3 Query: 267 NAYRVRNETLTIP 305 NAY ++N+T+T P Sbjct: 235 NAYLIKNQTITCP 247 >AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase protein. Length = 1143 Score = 22.6 bits (46), Expect = 3.6 Identities = 12/27 (44%), Positives = 13/27 (48%) Frame = -1 Query: 634 PLQQLELDHHQKALYSLESRPLQHVGQ 554 PL L L Q YS+ S P H GQ Sbjct: 885 PLLLLHLTPLQPRFYSISSSPDVHQGQ 911 >DQ869053-1|ABJ09600.1| 459|Apis mellifera capa-like receptor protein. Length = 459 Score = 21.8 bits (44), Expect = 6.3 Identities = 9/19 (47%), Positives = 11/19 (57%) Frame = -3 Query: 560 RSNKPTFPAASLSSVLRFL 504 + N P FP LS +L FL Sbjct: 197 KENMPEFPLYQLSCILFFL 215 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 192,675 Number of Sequences: 438 Number of extensions: 4841 Number of successful extensions: 22 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 22 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 20830365 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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