Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00296
(775 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g45290.1 68415.m05637 transketolase, putative strong similari... 34 0.091
At3g60750.1 68416.m06796 transketolase, putative strong similari... 33 0.28
At1g53490.1 68414.m06064 bZIP protein 30 2.0
At5g02920.1 68418.m00235 F-box family protein low similarity to ... 29 2.6
At1g18870.1 68414.m02349 isochorismate synthase, putative / isoc... 29 2.6
At4g33690.1 68417.m04785 expressed protein 29 3.4
At5g58730.1 68418.m07357 pfkB-type carbohydrate kinase family pr... 28 6.0
At3g63290.2 68416.m07114 expressed protein 28 6.0
At3g63290.1 68416.m07113 expressed protein 28 6.0
At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) / isoch... 28 6.0
At1g74710.1 68414.m08654 isochorismate synthase 1 (ICS1) / isoch... 28 6.0
At5g43310.1 68418.m05293 COP1-interacting protein-related contai... 28 7.9
>At2g45290.1 68415.m05637 transketolase, putative strong similarity
to transketolase 1 [Capsicum annuum] GI:3559814;
contains Pfam profiles PF02779: Transketolase, pyridine
binding domain, PF02780: Transketolase, C-terminal
domain, PF00456: Transketolase, thiamine diphosphate
binding domain
Length = 741
Score = 34.3 bits (75), Expect = 0.091
Identities = 19/45 (42%), Positives = 23/45 (51%)
Frame = +2
Query: 554 EVLGTFGGWSSKSTGAVLDDLRRSISGNGCISLSENVDKRLEILG 688
EV G W A DD SI G+ I+ +E+VDKR E LG
Sbjct: 243 EVCSLAGHWGLGKLIAFYDDNHISIDGDTDIAFTESVDKRFEALG 287
>At3g60750.1 68416.m06796 transketolase, putative strong similarity
to transketolase 1 [Capsicum annuum] GI:3559814;
contains Pfam profiles PF02779: Transketolase, pyridine
binding domain, PF02780: Transketolase, C-terminal
domain, PF00456: Transketolase, thiamine diphosphate
binding domain
Length = 741
Score = 32.7 bits (71), Expect = 0.28
Identities = 18/45 (40%), Positives = 22/45 (48%)
Frame = +2
Query: 554 EVLGTFGGWSSKSTGAVLDDLRRSISGNGCISLSENVDKRLEILG 688
E G W A DD SI G+ I+ +ENVD+R E LG
Sbjct: 243 EACSLAGHWGLGKLIAFYDDNHISIDGDTEIAFTENVDQRFEALG 287
>At1g53490.1 68414.m06064 bZIP protein
Length = 229
Score = 29.9 bits (64), Expect = 2.0
Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = -2
Query: 693 NPPRISRRLSTFSDSDIHPLPEI--ERRRSSRTAPVDLELQPPNVPSTSGNTDA 538
N I + S FS + P EI R+ SS + P D+ P +PS GN A
Sbjct: 135 NRETIRKDRSFFSPATPGPKDEIWPARQNSSNSGPFDISTDSPAIPSDLGNRRA 188
>At5g02920.1 68418.m00235 F-box family protein low similarity to
ribosomal RNA apurinic site specific lyase [Triticum
aestivum] GI:6505722; contains F-box domain Pfam:PF00646
Length = 345
Score = 29.5 bits (63), Expect = 2.6
Identities = 20/77 (25%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
Frame = -1
Query: 514 GRKQSNIKWEMPSHDDPLAIDYTV--PCSGMKDIYVGLANANPIDYYDLFLTPNILEYIT 341
G S+I++ M + D L++ ++ PC+ D + ++ ++ LTP+ + T
Sbjct: 109 GHVHSSIEFAMSHNVDNLSLAFSSFPPCNKFPDFFYTSSSLKRVELRSASLTPSCIVSWT 168
Query: 340 EQTNLYATQCILPSDSS 290
+L T+C L SD S
Sbjct: 169 SLRDLSLTRCNL-SDKS 184
>At1g18870.1 68414.m02349 isochorismate synthase, putative /
isochorismate mutase, putative similar to GI:17223087
Length = 512
Score = 29.5 bits (63), Expect = 2.6
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = -2
Query: 681 ISRRLSTFSDSDIHPLPEIERRRSSRTAPVDLELQPPNVPSTSGNTDAQI 532
++R +D+DI P+ + R + LQPP P+ GNT ++
Sbjct: 264 LARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQPPGAPAFIGNTPERL 313
>At4g33690.1 68417.m04785 expressed protein
Length = 281
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -2
Query: 654 DSDIHPLPEIERRRSSRTAPVDLELQPPNVPSTSGNTDAQISEQE 520
D + LPE E R+S T L +QP P TD+ S++E
Sbjct: 199 DEPLPCLPERELSRTSDTGDRKLVIQPERSPLLRRRTDSSSSDEE 243
>At5g58730.1 68418.m07357 pfkB-type carbohydrate kinase family
protein contains Pfam profile: PF00294 pfkB family
carbohydrate kinase
Length = 353
Score = 28.3 bits (60), Expect = 6.0
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +1
Query: 667 QTP*NPRRVPNLTVIVVTGYLCH 735
QT +PRR+PN V++V Y CH
Sbjct: 10 QTSHSPRRIPNRRVLIVGNY-CH 31
>At3g63290.2 68416.m07114 expressed protein
Length = 303
Score = 28.3 bits (60), Expect = 6.0
Identities = 12/43 (27%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +1
Query: 448 YNLWQEDHHDLA-FPILCWTVFFHPLLFRNLSISITRSTWHVW 573
+NLWQ+ H+D F +L +F P ++ S+ + S ++
Sbjct: 136 FNLWQQWHYDYGIFTVLTDPMFLSPYSYQEFSLMSSHSYLQIY 178
>At3g63290.1 68416.m07113 expressed protein
Length = 403
Score = 28.3 bits (60), Expect = 6.0
Identities = 12/43 (27%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +1
Query: 448 YNLWQEDHHDLA-FPILCWTVFFHPLLFRNLSISITRSTWHVW 573
+NLWQ+ H+D F +L +F P ++ S+ + S ++
Sbjct: 236 FNLWQQWHYDYGIFTVLTDPMFLSPYSYQEFSLMSSHSYLQIY 278
>At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) /
isochorismate mutase identical to GI:17223087 and
GB:AF078080; contains Pfam profile PF00425: chorismate
binding enzyme; contains TIGRfam profile TIGR00543:
isochorismate synthases; identical to cDNA isochorismate
synthase 1 precursor (ICS1) nuclear gene for plastid
product GI:17223086
Length = 622
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/53 (24%), Positives = 26/53 (49%)
Frame = -2
Query: 681 ISRRLSTFSDSDIHPLPEIERRRSSRTAPVDLELQPPNVPSTSGNTDAQISEQ 523
++R +D+DI P+ + + + LQPP P+ GNT ++ ++
Sbjct: 319 LARNSRIITDTDIDPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQR 371
>At1g74710.1 68414.m08654 isochorismate synthase 1 (ICS1) /
isochorismate mutase identical to GI:17223087 and
GB:AF078080; contains Pfam profile PF00425: chorismate
binding enzyme; contains TIGRfam profile TIGR00543:
isochorismate synthases; identical to cDNA isochorismate
synthase 1 precursor (ICS1) nuclear gene for plastid
product GI:17223086
Length = 569
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/53 (24%), Positives = 26/53 (49%)
Frame = -2
Query: 681 ISRRLSTFSDSDIHPLPEIERRRSSRTAPVDLELQPPNVPSTSGNTDAQISEQ 523
++R +D+DI P+ + + + LQPP P+ GNT ++ ++
Sbjct: 319 LARNSRIITDTDIDPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQR 371
>At5g43310.1 68418.m05293 COP1-interacting protein-related contains
similarity to COP1-Interacting Protein 7 (CIP7)
[Arabidopsis thaliana] GI:3327868
Length = 1237
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
Frame = -2
Query: 732 TQVPSDDNDSQIWNPPRISRRLSTFSDSDIHPLPEIER--RRSSRTAPVDLELQPPN 568
+Q+P+ QI N R S FSDS+ L ++R RR++ D + P N
Sbjct: 785 SQLPAQQTKKQILNKFSPGSRASKFSDSEPGSLSPLQRLPRRTTSLGSNDFQKFPKN 841
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,073,062
Number of Sequences: 28952
Number of extensions: 407694
Number of successful extensions: 1049
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1018
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1048
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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