BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00295 (392 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|... 128 2e-31 SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12... 128 2e-31 SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 27 1.4 SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces... 26 1.8 SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 26 1.8 SPCC1223.14 ||SPCC297.01|chorismate synthase |Schizosaccharomyce... 24 9.7 SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 24 9.7 >SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 128 bits (310), Expect = 2e-31 Identities = 56/87 (64%), Positives = 77/87 (88%) Frame = +1 Query: 1 AKATSDWKGLKITVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISL 180 AKAT DWKGL++TV+LT+QNRQA ++VVPSA+AL+I+ALKEP RDRKK KN+ H+GN+SL Sbjct: 47 AKATKDWKGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSL 106 Query: 181 EDVIGIAKIMRNRSMARYLSGSVKEFL 261 +++I +A+ MR +S+A+ LSG+VKE L Sbjct: 107 DEIIEVARTMRFKSLAKELSGTVKEIL 133 Score = 43.2 bits (97), Expect = 1e-05 Identities = 18/29 (62%), Positives = 24/29 (82%) Frame = +3 Query: 249 KRILGTAQSVGCTVEGRPPHDLIDDINSG 335 K ILGTA SVGCTV+G+ PHD+ +I++G Sbjct: 130 KEILGTAFSVGCTVDGKNPHDVQKEIDNG 158 >SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 128 bits (310), Expect = 2e-31 Identities = 56/87 (64%), Positives = 77/87 (88%) Frame = +1 Query: 1 AKATSDWKGLKITVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISL 180 AKAT DWKGL++TV+LT+QNRQA ++VVPSA+AL+I+ALKEP RDRKK KN+ H+GN+SL Sbjct: 47 AKATKDWKGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSL 106 Query: 181 EDVIGIAKIMRNRSMARYLSGSVKEFL 261 +++I +A+ MR +S+A+ LSG+VKE L Sbjct: 107 DEIIEVARTMRFKSLAKELSGTVKEIL 133 Score = 43.2 bits (97), Expect = 1e-05 Identities = 18/29 (62%), Positives = 24/29 (82%) Frame = +3 Query: 249 KRILGTAQSVGCTVEGRPPHDLIDDINSG 335 K ILGTA SVGCTV+G+ PHD+ +I++G Sbjct: 130 KEILGTAFSVGCTVDGKNPHDVQKEIDNG 158 >SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 874 Score = 26.6 bits (56), Expect = 1.4 Identities = 12/39 (30%), Positives = 19/39 (48%) Frame = -2 Query: 337 TPLLMSSIRSCGGLPSTVHPTDCAVPRILLLSQKGTGPL 221 TP+ M +CG +P +P + + +KGT PL Sbjct: 440 TPIPMVHCETCGAVPVPESELPVKLPDLDKIYEKGTSPL 478 >SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 26.2 bits (55), Expect = 1.8 Identities = 17/73 (23%), Positives = 31/73 (42%) Frame = +1 Query: 31 KITVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISLEDVIGIAKIM 210 K + Q ++ N + V ++ L LKE +D++K ++ S + A+ Sbjct: 23 KPSQQASIPNVELSSTVTSNSQVLNNDPLKETKKDKRKDRHFNWQQKFSNPKISASARKR 82 Query: 211 RNRSMARYLSGSV 249 RNR L +V Sbjct: 83 RNRKARENLKVNV 95 >SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual Length = 968 Score = 26.2 bits (55), Expect = 1.8 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = -3 Query: 144 LFTITRRLLKGSDDKGCCRGNNSYLGLSVLNCQLHS 37 +FT+ R LK + CR N + GLS C +S Sbjct: 805 VFTLPRNNLKSKTNTKKCRDNLNLSGLSSSTCNANS 840 >SPCC1223.14 ||SPCC297.01|chorismate synthase |Schizosaccharomyces pombe|chr 3|||Manual Length = 395 Score = 23.8 bits (49), Expect = 9.7 Identities = 9/12 (75%), Positives = 10/12 (83%) Frame = +3 Query: 270 QSVGCTVEGRPP 305 +SVGC VEG PP Sbjct: 19 KSVGCIVEGCPP 30 >SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 23.8 bits (49), Expect = 9.7 Identities = 9/20 (45%), Positives = 12/20 (60%) Frame = -1 Query: 356 FIHQWSNSAVDVINKIMWRP 297 +IH +N A VIN + W P Sbjct: 72 YIHTSANKARHVINVVRWTP 91 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,623,902 Number of Sequences: 5004 Number of extensions: 31517 Number of successful extensions: 77 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 73 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 77 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 130061696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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