BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00294
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 31 0.18
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.0
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 26 5.0
SPBC2F12.10 |||mitochondrial ribosomal protein subunit L35|Schiz... 26 6.6
SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M... 26 6.6
SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizos... 25 8.7
SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 8.7
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 25 8.7
>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
Wis4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1401
Score = 31.1 bits (67), Expect = 0.18
Identities = 22/87 (25%), Positives = 42/87 (48%), Gaps = 5/87 (5%)
Frame = +3
Query: 459 RLLHHFRSVHAPVFSSGYNLKENLQENH----LQFLQL-FTQYAKAADNGEELKSFQNLL 623
R+ + + +P S G++L N+ E++ L L+ F +G + F+ +
Sbjct: 515 RIKREYIKIASP--SPGWSLPTNVDEDYDNVLLDSLKFYFKLLTLKLSSGNKNLYFKEID 572
Query: 624 FLIRDWSYYDTHPYGSKGGDSLVAKRF 704
FL +W++ + H Y GGD +A +F
Sbjct: 573 FLENEWAFLNEHIYWINGGDIHMAGQF 599
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -3
Query: 335 SDPPRHVKPPRGSESSPKGLALSPFS 258
S+PPR + PP G ++P +P S
Sbjct: 639 SNPPRSLPPPSGQVNAPMSQTPNPIS 664
>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
Mde5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 26.2 bits (55), Expect = 5.0
Identities = 20/50 (40%), Positives = 24/50 (48%), Gaps = 6/50 (12%)
Frame = +1
Query: 271 NAKPLG-EDSEPLGGFTWRGGSER----NTVGLL-IWSEPIIKTLENGDK 402
N KP ED E GG WRG ++ +G IW PIIK +E K
Sbjct: 48 NPKPCNPEDREYCGG-NWRGIIDKLDYIQGMGFTAIWISPIIKNIEGRTK 96
>SPBC2F12.10 |||mitochondrial ribosomal protein subunit
L35|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +1
Query: 292 DSEPLGGFTWRGGSERNTVGLL 357
D EP+G WR G + + V LL
Sbjct: 330 DLEPVGAHLWRSGWDSDAVALL 351
>SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 407
Score = 25.8 bits (54), Expect = 6.6
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Frame = +1
Query: 202 KIVPAKLLFEISAFACF*GEKGDNAKPLGEDSEPLGGFTWRGGSERNTVGLLIWSEPIIK 381
+++P +LFE F E LG++S+ L G S N + SE I++
Sbjct: 277 QVIPLSVLFEEQLFMLTPEEAAQECASLGKNSQ-LSEIICAGYSALNLIHYFTASEKIVQ 335
Query: 382 --TLENGDK 402
T+ +G K
Sbjct: 336 AWTIADGSK 344
>SPAC2E1P3.05c |||fungal cellulose binding domain
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 197
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/32 (37%), Positives = 23/32 (71%)
Frame = -2
Query: 183 ATLNNTTGKSLTSSRFSTLCNATTSKSNEPSS 88
++++ ++ SL+SS FST+ ++T + S PSS
Sbjct: 159 SSVSTSSFTSLSSSGFSTVLSSTNTTSALPSS 190
>SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1317
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/23 (47%), Positives = 14/23 (60%), Gaps = 1/23 (4%)
Frame = +3
Query: 657 HPYGSKGG-DSLVAKRFEVTKDL 722
HPYG KGG D+ K + KD+
Sbjct: 1223 HPYGMKGGKDAECGKNIWIRKDI 1245
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 25.4 bits (53), Expect = 8.7
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -3
Query: 725 RKILRHLKSFSD 690
RK+LRH+K FSD
Sbjct: 128 RKVLRHMKEFSD 139
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,056,176
Number of Sequences: 5004
Number of extensions: 63991
Number of successful extensions: 197
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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