Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00294
(751 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46070.1 68418.m05665 guanylate-binding family protein contai... 38 0.007
At2g28240.1 68415.m03428 hydroxyproline-rich glycoprotein family... 33 0.27
At2g38840.1 68415.m04772 guanylate-binding family protein simila... 32 0.35
At3g05310.1 68416.m00579 GTP-binding protein-related low similar... 29 2.5
At5g45390.1 68418.m05578 ATP-dependent Clp protease proteolytic ... 29 3.3
At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE... 29 4.4
At5g45160.1 68418.m05544 root hair defective 3 GTP-binding (RHD3... 28 5.8
At2g38440.1 68415.m04721 expressed protein 28 5.8
At4g27595.1 68417.m03964 protein transport protein-related low s... 28 7.6
At4g11060.1 68417.m01797 single-strand-binding family protein co... 28 7.6
At3g03510.1 68416.m00349 phototropic-responsive NPH3 family prot... 28 7.6
>At5g46070.1 68418.m05665 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding
protein, C-terminal domain
Length = 1060
Score = 37.9 bits (84), Expect = 0.007
Identities = 23/63 (36%), Positives = 38/63 (60%)
Frame = +2
Query: 41 ASMSGTPREIQLLKYKDDGSFDLDVVALQSVLNRDDVKDLPVVLFSVAGPFRTGKSFLLN 220
++++G PR I+L+ + G F +D A+ ++ VK+ P+ + SV G R GKSF+LN
Sbjct: 33 SAVTGPPRPIRLVYCDEKGKFRMDPEAVATL---QLVKE-PIGVVSVCGRARQGKSFILN 88
Query: 221 YFL 229
L
Sbjct: 89 QLL 91
Score = 33.1 bits (72), Expect = 0.20
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = +1
Query: 343 TVGLLIWSEPIIKTLENGDKVAVLLMDTQGLFDSKTTM 456
T GL +WS PI +T +G + +LL+D++G+ TM
Sbjct: 107 TKGLWLWSSPIKRTALDGTEYNLLLLDSEGIDAYDQTM 144
>At2g28240.1 68415.m03428 hydroxyproline-rich glycoprotein family
protein
Length = 660
Score = 32.7 bits (71), Expect = 0.27
Identities = 22/80 (27%), Positives = 41/80 (51%), Gaps = 1/80 (1%)
Frame = -3
Query: 353 RPTVLRSDPPRHVKPPRGSESSPKGL-ALSPFSP*KQANADISKSSLAGTIFQCGMVQPR 177
RP+V ++P PPR S ++ + + + +P P A A+ + +L+ + C QP
Sbjct: 317 RPSVTAAEPMNSAAPPRPSVTAAEPMNSTAPPRPSVTA-AEATPPNLSAPLPHCNTPQPS 375
Query: 176 *ITQQASL*HHRDSAHSATP 117
I+QQA++ + +A P
Sbjct: 376 PISQQAAVESNTQMQSTALP 395
>At2g38840.1 68415.m04772 guanylate-binding family protein similar
to SP|Q01514 Interferon-induced guanylate-binding
protein 1 (Guanine nucleotide-binding protein 1)
(Interferon-gamma inducible protein MAG-1) {Mus
musculus}; contains Pfam profile PF02263:
Guanylate-binding protein, N-terminal domain
Length = 679
Score = 32.3 bits (70), Expect = 0.35
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2
Query: 161 PVVLFSVAGPFRTGKSFLLNYFL 229
P+ +V GP+R+GKSFLLN L
Sbjct: 59 PISAVAVIGPYRSGKSFLLNQLL 81
>At3g05310.1 68416.m00579 GTP-binding protein-related low similarity
to rac 1 protein [Physcomitrella patens] GI:7243743;
contains Pfam profile PF00036: EF hand (domain)
Length = 648
Score = 29.5 bits (63), Expect = 2.5
Identities = 24/62 (38%), Positives = 32/62 (51%), Gaps = 5/62 (8%)
Frame = +2
Query: 59 PREIQLLKY----KDDGSFDLDVVALQSVLNRDDVK-DLPVVLFSVAGPFRTGKSFLLNY 223
PR ++ L Y DD S + V + VL+R + K + VV V GP GKS LLN
Sbjct: 388 PRSLEYLMYIRFPSDDPSSAVRVTR-KRVLDRKEKKSERKVVQCFVFGPKNAGKSALLNQ 446
Query: 224 FL 229
F+
Sbjct: 447 FI 448
>At5g45390.1 68418.m05578 ATP-dependent Clp protease proteolytic
subunit (ClpP4) identical to nClpP4 GI:5360593 from
[Arabidopsis thaliana]
Length = 292
Score = 29.1 bits (62), Expect = 3.3
Identities = 15/47 (31%), Positives = 28/47 (59%)
Frame = -2
Query: 189 GPATLNNTTGKSLTSSRFSTLCNATTSKSNEPSSLYLSNWISLGVPL 49
G +L+++ SL SSR ++ +A++S +P++LYL + PL
Sbjct: 2 GTLSLSSSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPTKLISPPL 48
>At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE) /
sigma-like factor (SIG5) identical to RNA polymerase
sigma subunit SigE [Arabidopsis thaliana] GI:4972299,
sigma-like factor [Arabidopsis thaliana] GI:4033838;
contains Pfam profiles PF04545: Sigma-70, region 4,
PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2
Length = 517
Score = 28.7 bits (61), Expect = 4.4
Identities = 20/62 (32%), Positives = 34/62 (54%), Gaps = 6/62 (9%)
Frame = -3
Query: 503 TEDRSVDRAKMMEQSLIVVLLSNKPCVSINS--TATLSPF----SRVFMIGSDQIKRPTV 342
TED V+R K+ + VL + KP +S+NS + T F + V +G++ ++P +
Sbjct: 385 TEDEVVERLKITPERYREVLRAAKPVLSLNSKHSVTQEEFINGITDVDGVGANNSRQPAL 444
Query: 341 LR 336
LR
Sbjct: 445 LR 446
>At5g45160.1 68418.m05544 root hair defective 3 GTP-binding (RHD3)
family protein contains Pfam profile: PF05879 root hair
defective 3 GTP-binding protein (RHD3) family
Length = 834
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/49 (28%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
Frame = +2
Query: 92 DGSFDLDVVALQSVLNRDDVKD--LPVVLFSVAGPFRTGKSFLLNYFLR 232
DG+ + +V L + + + + D L + ++ GP +GKS LLN+ +
Sbjct: 14 DGNGEFNVKGLDNFVKKTKLSDCGLSYAVVAIMGPQSSGKSTLLNHLFK 62
>At2g38440.1 68415.m04721 expressed protein
Length = 1399
Score = 28.3 bits (60), Expect = 5.8
Identities = 20/61 (32%), Positives = 26/61 (42%)
Frame = -2
Query: 693 RRVSPPLYCHKGGCHNTTNHVSGRADFGTTSILLRYQLLSHTV*TTEGTEDDSPGDSLSG 514
R PP + NT +GR+ FG S Y S T + +D S G+ LSG
Sbjct: 423 RMEDPPQFSFSHSNGNTPVSENGRSSFGKKSTSYSY---SDT--ASISIDDQSDGEKLSG 477
Query: 513 C 511
C
Sbjct: 478 C 478
>At4g27595.1 68417.m03964 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 1212
Score = 27.9 bits (59), Expect = 7.6
Identities = 22/80 (27%), Positives = 35/80 (43%), Gaps = 2/80 (2%)
Frame = +3
Query: 513 NLKENL--QENHLQFLQLFTQYAKAADNGEELKSFQNLLFLIRDWSYYDTHPYGSKGGDS 686
NLK++L +EN L+ + + KA + LK LL L + W K ++
Sbjct: 955 NLKQSLLDKENELEGVFQANEELKAKE-ASSLKKIDELLHLEQSWIDKGNENQELKVREA 1013
Query: 687 LVAKRFEVTKDLSTDMVDYE 746
AKR E + ++D E
Sbjct: 1014 SAAKRIEELSKMKESLLDKE 1033
>At4g11060.1 68417.m01797 single-strand-binding family protein
contains Pfam domain PF00436: Single-strand binding
protein family
Length = 201
Score = 27.9 bits (59), Expect = 7.6
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +1
Query: 298 EPLGGFTWRGGSERNTVGLLIWSEPIIKTLENGDKVAVLLMDTQGLFDSK 447
+P G+ +RG G + P+ K L NG V + + T G+FD +
Sbjct: 61 DPRKGWGFRGVHRAIICGK-VGQAPLQKILRNGRTVTIFTVGTGGMFDQR 109
>At3g03510.1 68416.m00349 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 455
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/35 (34%), Positives = 24/35 (68%), Gaps = 2/35 (5%)
Frame = +2
Query: 62 REIQLLKYKDDGSFDLDVV--ALQSVLNRDDVKDL 160
+++Q+L +DGS+D++++ L+S + D V DL
Sbjct: 217 KDLQILSPTEDGSYDIELLKTILKSFYSNDSVPDL 251
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,195,482
Number of Sequences: 28952
Number of extensions: 345482
Number of successful extensions: 1132
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1082
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1129
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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