BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00291 (786 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 108 1e-24 SPAC2F7.05c |||translation initiation factor eIF5 |Schizosacchar... 36 0.009 SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pom... 28 1.7 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 3.0 SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|ch... 27 4.0 SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory su... 26 5.3 SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosa... 26 5.3 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 25 9.3 SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces ... 25 9.3 SPAC16E8.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.3 SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 25 9.3 >SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation factor eIF2 beta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 108 bits (259), Expect = 1e-24 Identities = 49/67 (73%), Positives = 56/67 (83%) Frame = +1 Query: 508 IIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQCETCGSRCSVASIKS 687 IIKGRFQQKQIENVLRRYI EYVTC TC+SPDTIL K+ R+FF+ CE CGS SV +IK+ Sbjct: 249 IIKGRFQQKQIENVLRRYIVEYVTCKTCKSPDTILTKENRIFFMTCEACGSVRSVQAIKT 308 Query: 688 GFQAVTG 708 G+QA G Sbjct: 309 GYQAQIG 315 Score = 94.3 bits (224), Expect = 2e-20 Identities = 46/87 (52%), Positives = 62/87 (71%) Frame = +2 Query: 257 DYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQ 436 DY Y ELL R F ++R NP + +G+K+K+ + PP V R G KKT FAN ++I K +HR Sbjct: 167 DYYYPELLNRFFTLLRTNNPEL-AGEKRKYTIVPPSVHREG-KKTIFANISDISKRMHRS 224 Query: 437 AKHLLDFLLAELGTSGSVDGNSQLLSK 517 H++ FL AELGTSGSVDG+S+L+ K Sbjct: 225 LDHVIQFLFAELGTSGSVDGSSRLIIK 251 >SPAC2F7.05c |||translation initiation factor eIF5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 395 Score = 35.5 bits (78), Expect = 0.009 Identities = 15/54 (27%), Positives = 30/54 (55%), Gaps = 2/54 (3%) Frame = +1 Query: 508 IIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTIL--QKDTRLFFLQCETCGSR 663 I+ G +++++L +I+ +V C +C++P+T L K + C+ CG R Sbjct: 76 IVNGAHDAGKLQDLLDVFIRRFVLCASCQNPETELSINKKDQTISYDCKACGYR 129 >SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 561 Score = 27.9 bits (59), Expect = 1.7 Identities = 18/58 (31%), Positives = 25/58 (43%), Gaps = 1/58 (1%) Frame = +3 Query: 423 LCIGRPSICWIFFWR-NWVLVVQWMETVNYYQRSLPTKTDRECFTSVYKRVCNMSYMP 593 LC+G PSI + WR VV ET+ ++ P+ FT K+ Y P Sbjct: 13 LCVGSPSITTLLGWRLQQSSVVTCQETIMFFNAPEPS---TNVFTIQSKKFGTRKYRP 67 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 27.1 bits (57), Expect = 3.0 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = -2 Query: 683 LMLATEQRDPHVSH*RKNNLVSFCKIVSG 597 +ML T+Q +P++ R+ L FC+ V G Sbjct: 680 IMLNTDQHNPNIKSQRRMTLDDFCRNVRG 708 >SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 26.6 bits (56), Expect = 4.0 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = +3 Query: 381 QRKPPLPILRKFVKLCIGRPSICWIFFWRNW 473 +R P L I F+K+C RP I +F W +W Sbjct: 211 KRLPNLRIFSNFIKVC--RPLIYMLFMW-HW 238 >SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory subunit Rpn12|Schizosaccharomyces pombe|chr 2|||Manual Length = 270 Score = 26.2 bits (55), Expect = 5.3 Identities = 10/21 (47%), Positives = 17/21 (80%) Frame = -1 Query: 438 ACRCKVLQISVKLAKEVFFVP 376 AC+ ++L++ V+LAK+ FVP Sbjct: 20 ACKKELLKLKVELAKQNLFVP 40 >SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 26.2 bits (55), Expect = 5.3 Identities = 17/53 (32%), Positives = 22/53 (41%), Gaps = 5/53 (9%) Frame = +1 Query: 376 WYKENLLCQFYGNL*NFASAGQAFVGFSSGGI-----GY*WFSGWKQSTIIKG 519 WYKE+ LC+ G G F G+ + G SGW+ II G Sbjct: 148 WYKESELCKRAGIFSASGLVGTMFAGYLQTAVHSSLNGKGGLSGWRWLFIIDG 200 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/37 (29%), Positives = 19/37 (51%) Frame = +3 Query: 195 VRGEDQENVEDEHGEWVGSDMITHMTNF*NEFLILCG 305 V G D + D H G+ +I++ +N+ E+ L G Sbjct: 132 VSGSDPVKMFDRHSSLNGTQIISYKSNYNEEWFTLIG 168 >SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1427 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = -1 Query: 609 NCIRGAACMTCYILFYIPT*NILYLFLLEATF 514 N R A C +LFY+ ++ LFL++ F Sbjct: 15 NFFRNATLDQCLLLFYLSLFSLTNLFLIQKLF 46 >SPAC16E8.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 269 Score = 25.4 bits (53), Expect = 9.3 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = -1 Query: 486 EPLVPNSARRKSNKCLACRCKVLQISVKLAKEVFF 382 EPL N + NK L C+ K + KLA ++ + Sbjct: 22 EPLEANLFGKLHNKLLICKAKKENLEKKLAYQMMY 56 >SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 1473 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +1 Query: 484 FSGWKQSTIIKGRFQQKQIENVLRRYIKEY 573 F G + + + + Q+K+I N+LR IK Y Sbjct: 1403 FYGQRLNKLYRSSVQEKEILNLLRPLIKRY 1432 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,057,272 Number of Sequences: 5004 Number of extensions: 63445 Number of successful extensions: 203 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 201 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 381366860 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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