Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00291
(786 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20920.2 68418.m02485 eukaryotic translation initiation facto... 118 6e-27
At5g20920.1 68418.m02484 eukaryotic translation initiation facto... 118 6e-27
At3g07920.1 68416.m00967 eukaryotic translation initiation facto... 80 1e-15
At5g01940.1 68418.m00113 eukaryotic translation initiation facto... 69 3e-12
At1g36730.1 68414.m04569 eukaryotic translation initiation facto... 43 3e-04
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 40 0.002
At3g42100.1 68416.m04322 AT hook motif-containing protein-relate... 29 4.6
At5g13840.1 68418.m01618 WD-40 repeat family protein contains 6 ... 28 6.1
At3g54440.1 68416.m06023 glycoside hydrolase family 2 protein si... 28 8.1
>At5g20920.2 68418.m02485 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 267
Score = 118 bits (283), Expect = 6e-27
Identities = 52/87 (59%), Positives = 70/87 (80%)
Frame = +2
Query: 257 DYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQ 436
DY YDELL RVF+I+RE NP + +G +++ +MRPPQV+R GTKKT F NF ++CKT+HRQ
Sbjct: 114 DYIYDELLGRVFNILRENNPEL-AGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQ 172
Query: 437 AKHLLDFLLAELGTSGSVDGNSQLLSK 517
H++ +LLAELGTSGS+DG +L+ K
Sbjct: 173 PDHVMQYLLAELGTSGSLDGQQRLVVK 199
Score = 93.5 bits (222), Expect = 1e-19
Identities = 41/68 (60%), Positives = 51/68 (75%)
Frame = +1
Query: 496 KQSTIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQCETCGSRCSVA 675
+Q ++KGRF K E +LRRYI +YV C C+SPDTIL K+ RLFFL+CE CGS+ SVA
Sbjct: 193 QQRLVVKGRFAPKNFEGILRRYITDYVICLGCKSPDTILSKENRLFFLRCEKCGSQRSVA 252
Query: 676 SIKSGFQA 699
IK+GF A
Sbjct: 253 PIKTGFVA 260
>At5g20920.1 68418.m02484 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 268
Score = 118 bits (283), Expect = 6e-27
Identities = 52/87 (59%), Positives = 70/87 (80%)
Frame = +2
Query: 257 DYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQ 436
DY YDELL RVF+I+RE NP + +G +++ +MRPPQV+R GTKKT F NF ++CKT+HRQ
Sbjct: 115 DYIYDELLGRVFNILRENNPEL-AGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQ 173
Query: 437 AKHLLDFLLAELGTSGSVDGNSQLLSK 517
H++ +LLAELGTSGS+DG +L+ K
Sbjct: 174 PDHVMQYLLAELGTSGSLDGQQRLVVK 200
Score = 93.5 bits (222), Expect = 1e-19
Identities = 41/68 (60%), Positives = 51/68 (75%)
Frame = +1
Query: 496 KQSTIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQCETCGSRCSVA 675
+Q ++KGRF K E +LRRYI +YV C C+SPDTIL K+ RLFFL+CE CGS+ SVA
Sbjct: 194 QQRLVVKGRFAPKNFEGILRRYITDYVICLGCKSPDTILSKENRLFFLRCEKCGSQRSVA 253
Query: 676 SIKSGFQA 699
IK+GF A
Sbjct: 254 PIKTGFVA 261
>At3g07920.1 68416.m00967 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 169
Score = 80.2 bits (189), Expect = 1e-15
Identities = 42/84 (50%), Positives = 56/84 (66%), Gaps = 4/84 (4%)
Frame = +2
Query: 272 ELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRI----GTKKTSFANFTEICKTLHRQA 439
E+L RVF+I+RE +P +V G I+ PPQV+R GTKKT F NF + CKT+HR
Sbjct: 18 EILRRVFNILRENSPELV-GIWLLTIIWPPQVLREETAKGTKKTVFVNFMDYCKTMHRNP 76
Query: 440 KHLLDFLLAELGTSGSVDGNSQLL 511
H++ FLLAELGT G +D +L+
Sbjct: 77 DHVMAFLLAELGTRGKLDDQQRLV 100
Score = 60.9 bits (141), Expect = 9e-10
Identities = 24/50 (48%), Positives = 36/50 (72%)
Frame = +1
Query: 496 KQSTIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQC 645
+Q +++GRF Q+ E++LR YI +YV C C+S + IL K+ RLFFL+C
Sbjct: 96 QQRLVVRGRFTQRDFESLLRGYILDYVMCIPCKSTEAILSKENRLFFLRC 145
>At5g01940.1 68418.m00113 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 231
Score = 69.3 bits (162), Expect = 3e-12
Identities = 36/94 (38%), Positives = 61/94 (64%), Gaps = 1/94 (1%)
Frame = +2
Query: 257 DYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQ 436
DY Y ELL VFD +RE++ VS ++ + +M PPQ++ GT T NF ++C+T+HR+
Sbjct: 72 DYGYKELLSMVFDRLREEDVE-VSTERPRTVMMPPQLLAEGTI-TVCLNFADLCRTMHRK 129
Query: 437 AKHLLDFLLAELGTSGSVDGNSQL-LSKVASNKN 535
H++ FLLA++ T S++ +L + + S+K+
Sbjct: 130 PDHVMKFLLAQMETKVSLNKQQRLEIKGLVSSKD 163
Score = 47.2 bits (107), Expect = 1e-05
Identities = 25/65 (38%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
Frame = +1
Query: 496 KQSTIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTIL-QKDTRLFFLQCETCGSRCSV 672
+Q IKG K + V R+YI +V C C+SPDT L ++ LF L+CET +
Sbjct: 150 QQRLEIKGLVSSKDFQAVFRKYIDAFVICVCCKSPDTALAEEGNGLFNLRCETGKDEDKL 209
Query: 673 ASIKS 687
S +S
Sbjct: 210 VSFES 214
>At1g36730.1 68414.m04569 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 439
Score = 42.7 bits (96), Expect = 3e-04
Identities = 17/53 (32%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = +1
Query: 502 STIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDT-ILQKDTRLFFLQCETCG 657
++++ G ++ +L +IK+YV C+ C +P+T IL T++ L+C CG
Sbjct: 75 TSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQLKCAACG 127
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 39.9 bits (89), Expect = 0.002
Identities = 15/53 (28%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = +1
Query: 502 STIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDT-ILQKDTRLFFLQCETCG 657
++++ G ++ +L +IK++V C+ C +P+T I+ T++ L+C CG
Sbjct: 75 TSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVNLKCAACG 127
>At3g42100.1 68416.m04322 AT hook motif-containing protein-related
very low similarity to SP|Q9UUA2 DNA repair and
recombination protein pif1, mitochondrial precursor
{Schizosaccharomyces pombe}; weak hit to Pfam profile
PF02178: AT hook motif
Length = 1752
Score = 28.7 bits (61), Expect = 4.6
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -1
Query: 495 PSTEPLVPNSARRKSNKCLACR 430
P E +PNSA++K KC++ R
Sbjct: 590 PGIEKCLPNSAKKKKKKCISMR 611
>At5g13840.1 68418.m01618 WD-40 repeat family protein contains 6
WD-40 repeats (PF00400); similar to Fzr1
(GI:6463679){Homo sapiens}
Length = 481
Score = 28.3 bits (60), Expect = 6.1
Identities = 21/47 (44%), Positives = 27/47 (57%), Gaps = 6/47 (12%)
Frame = +2
Query: 578 HVIHAAPLIQ--FYRKILDYSSFNVRHVGL---VVQWLALN-LVSKL 700
H + AP +Q FY ++D+SS NV VGL V W A N V+KL
Sbjct: 163 HKVLDAPSLQDDFYLNVVDWSSQNVLAVGLGTCVYLWTASNSKVTKL 209
>At3g54440.1 68416.m06023 glycoside hydrolase family 2 protein
similar to beta-galactosidase (lactase) from Alteromonas
haloplanktis [SP|P81650]; contains Pfam glycoside
hydrolase domains PF02836, PF02837, PF02929, PF02930
Length = 1107
Score = 27.9 bits (59), Expect = 8.1
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = -2
Query: 536 CFCWKRPLIIVDCFHPLNH*YPIPPEENPTNAWPADAKFYKFP*NWQRRFSLYQF 372
C + RP I + +P + P PE+NPT + +++ P W+ R L F
Sbjct: 130 CHGFDRP-IYTNVVYPFPNDPPYVPEDNPTGCY---RTYFQIPKEWKDRRILLHF 180
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,834,289
Number of Sequences: 28952
Number of extensions: 330943
Number of successful extensions: 1098
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1055
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1093
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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