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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00289
         (710 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc...    42   1e-04
SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa...    38   0.001
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ...    28   1.1  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    26   4.6  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    26   4.6  
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc...    26   4.6  
SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone oxidase|Schizosacch...    26   6.1  
SPBC1718.02 |hop1||linear element associated protein Hop1|Schizo...    25   8.1  

>SPBC409.06 |uch2||ubiquitin C-terminal hydrolase
           Uch2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 300

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 18/41 (43%), Positives = 27/41 (65%)
 Frame = +1

Query: 583 EDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTSQETLLE 705
           ED V +HFI++ + +   YELDG +A P+NHG  ++E   E
Sbjct: 154 EDEV-YHFIAYTNINNVFYELDGLQAAPINHGSCTKEEFAE 193



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 20/53 (37%), Positives = 33/53 (62%)
 Frame = +2

Query: 347 NIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEAKGLDATARGK 505
           NIF+ KQ I+NAC T AL+  + N++D I+L    + +F + +K L    +G+
Sbjct: 71  NIFFAKQVINNACATQALLSVLLNHSDEIDLGT-TLSEFKDFSKTLPPELKGE 122


>SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase
           Uch1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 222

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 18/42 (42%), Positives = 29/42 (69%)
 Frame = +3

Query: 123 PLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPV 248
           PLE+ P+VL  +LQK+GV +  ++ D+  L+ E   ++PRPV
Sbjct: 4   PLENTPEVLEPYLQKIGVQDA-SVFDLFSLE-EIPEYIPRPV 43



 Score = 37.5 bits (83), Expect = 0.002
 Identities = 28/95 (29%), Positives = 46/95 (48%), Gaps = 1/95 (1%)
 Frame = +2

Query: 242 PCAFLMLLFPISDAYENHKKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANN 421
           P   L+ +FP S     +K +    IL K    S  + +  Q I NACGTI L+H+V+N 
Sbjct: 42  PVHALLFVFPSSGTKTIYKGSR---ILPKD---SDKVLWYPQTIPNACGTIGLLHAVSNG 95

Query: 422 TDIIELSDG-HMQKFLNEAKGLDATARGKLWKSLK 523
               ++++   ++  +  A+G     R KL +  K
Sbjct: 96  ELRRKVNENDFIKSLIRTAEGSSIEERAKLIEDSK 130



 Score = 31.5 bits (68), Expect = 0.12
 Identities = 23/70 (32%), Positives = 30/70 (42%), Gaps = 5/70 (7%)
 Frame = +1

Query: 505 IVEKSEGIINAHKELAQEGQTNTPSAED-PVNHHFISFVH----KDGALYELDGRKAFPV 669
           ++E S+ +   H   A        S ED   + HFI FV      D   YELDGR+  PV
Sbjct: 125 LIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYELDGRQEGPV 184

Query: 670 NHGPTSQETL 699
            H     + L
Sbjct: 185 QHSEIESDLL 194


>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 639

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 17/62 (27%), Positives = 28/62 (45%)
 Frame = +1

Query: 517 SEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTSQET 696
           S  ++    ELA +  TNT      V   ++++V  +   + L   K F   H P+S E 
Sbjct: 126 SRALLEQFAELASK--TNTSHMIHQVYDQYLNYVVLESDFFSLQLPKIFHTFHNPSSDEA 183

Query: 697 LL 702
           L+
Sbjct: 184 LI 185


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +1

Query: 580  AEDPVNHHFISFVHKDGALYELDGR 654
            AE+ V  HF+    KD  LY++D R
Sbjct: 1099 AEESVKRHFLRLWLKDNGLYDVDIR 1123


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 9/28 (32%), Positives = 20/28 (71%)
 Frame = +1

Query: 529 INAHKELAQEGQTNTPSAEDPVNHHFIS 612
           ++A + L ++ +++T + E+PV+HH  S
Sbjct: 337 LSARRALFEKKESSTKNVENPVSHHLKS 364


>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
           Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 934

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 13/52 (25%), Positives = 22/52 (42%)
 Frame = +1

Query: 511 EKSEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFP 666
           +  +G I   K  A + +    +  + ++H   S VH+D  L   DG    P
Sbjct: 43  DDEDGTIENSKVPASKSKVQKRNESEDISHSLPSIVHEDDKLVGSDGVSTTP 94


>SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone
           oxidase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 461

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 18/55 (32%), Positives = 25/55 (45%), Gaps = 8/55 (14%)
 Frame = +3

Query: 96  TEMATETLVPLESNPDVLNKFLQ-------KLGVPNKWNI-VDVMGLDPETLSWV 236
           ++  +E  +PLES PD L K +        K+G    W I V V    PE   W+
Sbjct: 318 SQHVSEWGIPLESAPDALEKLINYTVDDAGKIGAYTHWPIEVRVCAPTPEDECWL 372


>SPBC1718.02 |hop1||linear element associated protein
           Hop1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 528

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = +2

Query: 437 LSDGHMQKFLNEAKGLDATARGKLWKSLKASLMLTRNWLKK 559
           LS G  +K  NE++GL         + +K S     NWLKK
Sbjct: 282 LSQGKCEKMQNESRGLREIKNNNPCEEVKKS-----NWLKK 317


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,992,976
Number of Sequences: 5004
Number of extensions: 61158
Number of successful extensions: 174
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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