BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00289 (710 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc... 42 1e-04 SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa... 38 0.001 SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 28 1.1 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 26 4.6 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 4.6 SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 26 4.6 SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone oxidase|Schizosacch... 26 6.1 SPBC1718.02 |hop1||linear element associated protein Hop1|Schizo... 25 8.1 >SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosaccharomyces pombe|chr 2|||Manual Length = 300 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/41 (43%), Positives = 27/41 (65%) Frame = +1 Query: 583 EDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTSQETLLE 705 ED V +HFI++ + + YELDG +A P+NHG ++E E Sbjct: 154 EDEV-YHFIAYTNINNVFYELDGLQAAPINHGSCTKEEFAE 193 Score = 37.1 bits (82), Expect = 0.002 Identities = 20/53 (37%), Positives = 33/53 (62%) Frame = +2 Query: 347 NIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEAKGLDATARGK 505 NIF+ KQ I+NAC T AL+ + N++D I+L + +F + +K L +G+ Sbjct: 71 NIFFAKQVINNACATQALLSVLLNHSDEIDLGT-TLSEFKDFSKTLPPELKGE 122 >SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosaccharomyces pombe|chr 1|||Manual Length = 222 Score = 37.9 bits (84), Expect = 0.001 Identities = 18/42 (42%), Positives = 29/42 (69%) Frame = +3 Query: 123 PLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPV 248 PLE+ P+VL +LQK+GV + ++ D+ L+ E ++PRPV Sbjct: 4 PLENTPEVLEPYLQKIGVQDA-SVFDLFSLE-EIPEYIPRPV 43 Score = 37.5 bits (83), Expect = 0.002 Identities = 28/95 (29%), Positives = 46/95 (48%), Gaps = 1/95 (1%) Frame = +2 Query: 242 PCAFLMLLFPISDAYENHKKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANN 421 P L+ +FP S +K + IL K S + + Q I NACGTI L+H+V+N Sbjct: 42 PVHALLFVFPSSGTKTIYKGSR---ILPKD---SDKVLWYPQTIPNACGTIGLLHAVSNG 95 Query: 422 TDIIELSDG-HMQKFLNEAKGLDATARGKLWKSLK 523 ++++ ++ + A+G R KL + K Sbjct: 96 ELRRKVNENDFIKSLIRTAEGSSIEERAKLIEDSK 130 Score = 31.5 bits (68), Expect = 0.12 Identities = 23/70 (32%), Positives = 30/70 (42%), Gaps = 5/70 (7%) Frame = +1 Query: 505 IVEKSEGIINAHKELAQEGQTNTPSAED-PVNHHFISFVH----KDGALYELDGRKAFPV 669 ++E S+ + H A S ED + HFI FV D YELDGR+ PV Sbjct: 125 LIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYELDGRQEGPV 184 Query: 670 NHGPTSQETL 699 H + L Sbjct: 185 QHSEIESDLL 194 >SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces pombe|chr 3|||Manual Length = 639 Score = 28.3 bits (60), Expect = 1.1 Identities = 17/62 (27%), Positives = 28/62 (45%) Frame = +1 Query: 517 SEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTSQET 696 S ++ ELA + TNT V ++++V + + L K F H P+S E Sbjct: 126 SRALLEQFAELASK--TNTSHMIHQVYDQYLNYVVLESDFFSLQLPKIFHTFHNPSSDEA 183 Query: 697 LL 702 L+ Sbjct: 184 LI 185 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 26.2 bits (55), Expect = 4.6 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +1 Query: 580 AEDPVNHHFISFVHKDGALYELDGR 654 AE+ V HF+ KD LY++D R Sbjct: 1099 AEESVKRHFLRLWLKDNGLYDVDIR 1123 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 26.2 bits (55), Expect = 4.6 Identities = 9/28 (32%), Positives = 20/28 (71%) Frame = +1 Query: 529 INAHKELAQEGQTNTPSAEDPVNHHFIS 612 ++A + L ++ +++T + E+PV+HH S Sbjct: 337 LSARRALFEKKESSTKNVENPVSHHLKS 364 >SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 934 Score = 26.2 bits (55), Expect = 4.6 Identities = 13/52 (25%), Positives = 22/52 (42%) Frame = +1 Query: 511 EKSEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFP 666 + +G I K A + + + + ++H S VH+D L DG P Sbjct: 43 DDEDGTIENSKVPASKSKVQKRNESEDISHSLPSIVHEDDKLVGSDGVSTTP 94 >SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 25.8 bits (54), Expect = 6.1 Identities = 18/55 (32%), Positives = 25/55 (45%), Gaps = 8/55 (14%) Frame = +3 Query: 96 TEMATETLVPLESNPDVLNKFLQ-------KLGVPNKWNI-VDVMGLDPETLSWV 236 ++ +E +PLES PD L K + K+G W I V V PE W+ Sbjct: 318 SQHVSEWGIPLESAPDALEKLINYTVDDAGKIGAYTHWPIEVRVCAPTPEDECWL 372 >SPBC1718.02 |hop1||linear element associated protein Hop1|Schizosaccharomyces pombe|chr 2|||Manual Length = 528 Score = 25.4 bits (53), Expect = 8.1 Identities = 15/41 (36%), Positives = 20/41 (48%) Frame = +2 Query: 437 LSDGHMQKFLNEAKGLDATARGKLWKSLKASLMLTRNWLKK 559 LS G +K NE++GL + +K S NWLKK Sbjct: 282 LSQGKCEKMQNESRGLREIKNNNPCEEVKKS-----NWLKK 317 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,992,976 Number of Sequences: 5004 Number of extensions: 61158 Number of successful extensions: 174 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 159 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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