Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00289
         (710 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase, ...    60   1e-09
At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase f...    42   3e-04
At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase f...    37   0.015
At1g65010.1 68414.m07368 expressed protein similar to endosome-a...    33   0.25 
At1g74530.2 68414.m08635 expressed protein                             30   1.3  
At1g74530.1 68414.m08634 expressed protein                             30   1.3  
At1g60770.1 68414.m06841 pentatricopeptide (PPR) repeat-containi...    29   2.3  
At3g12760.1 68416.m01593 expressed protein similar to RP42 prote...    29   4.0  
At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1) ide...    27   9.3  
At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family pro...    27   9.3  

>At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase,
           putative / ubiquitin thiolesterase, putative similar to
           SP|Q9JKB1 Ubiquitin carboxyl-terminal hydrolase isozyme
           L3 (EC 3.4.19.12) (UCH- L3) (Ubiquitin thiolesterase L3)
           {Mus musculus}; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 234

 Score = 60.5 bits (140), Expect = 1e-09
 Identities = 30/89 (33%), Positives = 48/89 (53%), Gaps = 1/89 (1%)
 Frame = +2

Query: 242 PCAFLMLLFPISDAYENHKKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANN 421
           P   ++ L+PI+   E  +  ++ EI  K    S  +++MKQ + NACGTI L+H++ N 
Sbjct: 56  PVLAVLFLYPITKKSEEERIEQDKEIKEKVH--SDKVYFMKQTVGNACGTIGLLHAIGNI 113

Query: 422 TDIIELSDG-HMQKFLNEAKGLDATARGK 505
           T  I+LSDG  + +F      +    R K
Sbjct: 114 TSEIKLSDGSFLDRFFKSTANMTPMERAK 142



 Score = 55.2 bits (127), Expect = 4e-08
 Identities = 30/67 (44%), Positives = 41/67 (61%)
 Frame = +1

Query: 508 VEKSEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTS 687
           +E    I +AH      G  +TP++ED  + HFI     +G LYELDGRKA P++HG +S
Sbjct: 144 LENDSQIEDAHSVAVIAG--DTPASED-ADTHFICLACVEGELYELDGRKAGPISHGASS 200

Query: 688 QETLLED 708
             TLL+D
Sbjct: 201 PATLLKD 207



 Score = 51.6 bits (118), Expect = 5e-07
 Identities = 25/46 (54%), Positives = 34/46 (73%), Gaps = 1/46 (2%)
 Frame = +3

Query: 120 VPLESNPDVLNKFLQKLGV-PNKWNIVDVMGLDPETLSWVPRPVLS 254
           +PLESNPDV+N++L  LG+ P++    DV GLD E L  VP+PVL+
Sbjct: 14  LPLESNPDVMNQYLWGLGLAPDEAECNDVYGLDDELLEMVPKPVLA 59


>At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase
           family 1 protein similar to 26S proteasome regulatory
           complex subunit p37A [Drosophila melanogaster]
           GI:6434962; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 330

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 22/62 (35%), Positives = 33/62 (53%), Gaps = 4/62 (6%)
 Frame = +1

Query: 508 VEKSEGIINAHKELAQEG----QTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNH 675
           +  S+ I  AH   A+      +    + +D   +HFIS++  DG LYELDG K  P++ 
Sbjct: 122 INNSDSIRAAHNSFARPEPFVPEEQKAATKDDDVYHFISYIPVDGVLYELDGLKEGPISL 181

Query: 676 GP 681
           GP
Sbjct: 182 GP 183



 Score = 28.7 bits (61), Expect = 4.0
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = +2

Query: 332 QEVSGNIFYMKQNISNACGTIALVHSVANNTDI 430
           Q+   N+F+  Q I+NAC T A++  + N+ ++
Sbjct: 65  QDQVSNLFFANQVINNACATQAILAILLNSPEV 97


>At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase
           family 1 protein similar to 26S proteasome regulatory
           complex subunit p37A [Drosophila melanogaster]
           GI:6434962; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 334

 Score = 36.7 bits (81), Expect = 0.015
 Identities = 16/32 (50%), Positives = 22/32 (68%)
 Frame = +1

Query: 583 EDPVNHHFISFVHKDGALYELDGRKAFPVNHG 678
           ED   +H+IS++  DG LYELDG K  P++ G
Sbjct: 157 EDDDVYHYISYLPVDGILYELDGLKEGPISLG 188



 Score = 30.7 bits (66), Expect = 1.00
 Identities = 23/72 (31%), Positives = 39/72 (54%), Gaps = 5/72 (6%)
 Frame = +2

Query: 284 YENHKKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDI---IELSD--G 448
           Y+   + +EN ++    E + N F+  Q I+NAC T A++  + N++ I    ELS+   
Sbjct: 51  YKWRPEEKENRVVIT--EPNPNFFFASQIINNACATQAILSVLMNSSSIDIGSELSELKQ 108

Query: 449 HMQKFLNEAKGL 484
             ++F  E KGL
Sbjct: 109 FAKEFPPELKGL 120


>At1g65010.1 68414.m07368 expressed protein similar to
           endosome-associated protein (GI:1016368) [Homo sapiens];
           similar to Centromeric protein E (CENP-E protein)
           (Swiss-Prot:Q02224) [Homo sapiens]
          Length = 1318

 Score = 32.7 bits (71), Expect = 0.25
 Identities = 28/110 (25%), Positives = 52/110 (47%), Gaps = 7/110 (6%)
 Frame = +2

Query: 296 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEA 475
           KK+EE E  S  +EVS  +  +K++  +AC       S+ NN  + E    ++Q+ L EA
Sbjct: 522 KKSEE-ENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETLGEA 580

Query: 476 KGLDATARGKL------WKSLKASLMLTRNWLKKA-KQIHQVLKIPSTII 604
           K      +  L       K++ A +   R W     ++I ++ K+  +++
Sbjct: 581 KAESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELSKVKESLV 630


>At1g74530.2 68414.m08635 expressed protein 
          Length = 262

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 21/59 (35%), Positives = 28/59 (47%), Gaps = 5/59 (8%)
 Frame = +1

Query: 547 LAQEGQTNTPSAEDPVNHHFISFVHKDGALY---ELDGRKAFPV--NHGPTSQETLLED 708
           L Q G  N+PS   P +  F+S+  +   LY   E D ++  PV    G TSQ   L D
Sbjct: 31  LFQMGIRNSPSTSSPSSDSFVSYAEQSTRLYNKMEQDLQENGPVFLKQGETSQSLSLSD 89


>At1g74530.1 68414.m08634 expressed protein 
          Length = 318

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 21/59 (35%), Positives = 28/59 (47%), Gaps = 5/59 (8%)
 Frame = +1

Query: 547 LAQEGQTNTPSAEDPVNHHFISFVHKDGALY---ELDGRKAFPV--NHGPTSQETLLED 708
           L Q G  N+PS   P +  F+S+  +   LY   E D ++  PV    G TSQ   L D
Sbjct: 31  LFQMGIRNSPSTSSPSSDSFVSYAEQSTRLYNKMEQDLQENGPVFLKQGETSQSLSLSD 89


>At1g60770.1 68414.m06841 pentatricopeptide (PPR) repeat-containing
           protein low similarity to DNA-binding protein [Triticum
           aestivum] GI:6958202; contains Pfam profile PF01535: PPR
           repeat
          Length = 491

 Score = 29.5 bits (63), Expect = 2.3
 Identities = 11/21 (52%), Positives = 15/21 (71%)
 Frame = +1

Query: 487 CYCSW*IVEKSEGIINAHKEL 549
           CYC   + EK+EG++N  KEL
Sbjct: 132 CYCKELLTEKAEGLLNKMKEL 152


>At3g12760.1 68416.m01593 expressed protein similar to RP42 protein
           [Homo sapiens] GI:9896486; contains Pfam profile
           PF00627: UBA/TS-N domain, PF03556: Domain of unknown
           function (DUF298)
          Length = 250

 Score = 28.7 bits (61), Expect = 4.0
 Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 5/54 (9%)
 Frame = -1

Query: 278 QKLGKVALRKHRARYPRKRFRIKS----HDIYNIPFVWYAELLQK-LVQDIGIG 132
           Q LG  ++ K + + P  R  +K     H+IYN  F W  E  QK L  D  IG
Sbjct: 121 QALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGWAKEKGQKSLALDTAIG 174


>At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1)
           identical to RNA-dependent RNA polymerase [Arabidopsis
           thaliana] gi|8248473|gb|AAF74208
          Length = 1196

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 10/26 (38%), Positives = 15/26 (57%)
 Frame = +1

Query: 568 NTPSAEDPVNHHFISFVHKDGALYEL 645
           N P   +PV+ HF    HK+G  +E+
Sbjct: 305 NEPCFGEPVSDHFFCIHHKEGISFEI 330


>At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family protein
            similar to ubiquitin-conjugating enzyme GB:3319990 from
            [Mus musculus]; contains Pfam profile PF00179:
            Ubiquitin-conjugating enzyme
          Length = 1163

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
 Frame = +2

Query: 521  KASLMLTRNWLKKAKQIHQVL--KIPSTIILSALSIKME 631
            KAS   ++NW+KK +   ++L   +P TI + A   +M+
Sbjct: 909  KASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMD 947


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,287,811
Number of Sequences: 28952
Number of extensions: 307056
Number of successful extensions: 775
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 753
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 774
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)