BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00283 (773 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 31 0.24 SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 29 0.98 SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 29 0.98 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.0 SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 27 3.0 SPAC1039.08 |||serine acetyltransferase |Schizosaccharomyces pom... 27 3.9 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 5.2 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 26 6.9 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 26 6.9 SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces ... 26 6.9 SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.1 SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 25 9.1 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 30.7 bits (66), Expect = 0.24 Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 2/64 (3%) Frame = +3 Query: 510 TSSKITSRANSNSYSTNR*LSSSANITIKLSNWMLTLASTTTA*PGET--EKTTPATMSA 683 TSS ++S A SNS +++ SSS N T + +++ST ++ T TT AT ++ Sbjct: 209 TSSSLSSTAASNSATSSSLASSSLNSTTSATATSSSISSTVSSSTPLTSSNSTTAATSAS 268 Query: 684 GNSS 695 SS Sbjct: 269 ATSS 272 Score = 28.3 bits (60), Expect = 1.3 Identities = 17/62 (27%), Positives = 33/62 (53%) Frame = +3 Query: 510 TSSKITSRANSNSYSTNR*LSSSANITIKLSNWMLTLASTTTA*PGETEKTTPATMSAGN 689 TSS ++S A SNS +++ SSS N T + +L+ST + + +++++ Sbjct: 177 TSSSLSSTAASNSATSSSLASSSLNSTTSATATSSSLSSTAASNSATSSSLASSSLNSTT 236 Query: 690 SS 695 S+ Sbjct: 237 SA 238 Score = 25.4 bits (53), Expect = 9.1 Identities = 21/67 (31%), Positives = 37/67 (55%), Gaps = 4/67 (5%) Frame = +3 Query: 510 TSSKITSRANSNSYSTNR*LSSSANITIKLSNWMLTLASTTTA--*PGETEKTTPA--TM 677 TSS + S + ++S + ++SS+ + +++ L +STT++ T TT A T Sbjct: 115 TSSSLASSSTTSSSLASSSITSSSLASSSITSSSLASSSTTSSSLASSSTNSTTSATPTS 174 Query: 678 SAGNSSL 698 SA +SSL Sbjct: 175 SATSSSL 181 >SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1428 Score = 28.7 bits (61), Expect = 0.98 Identities = 17/71 (23%), Positives = 36/71 (50%), Gaps = 5/71 (7%) Frame = +2 Query: 158 EGYVVAYGYSENSDDIQNLERELAKTAYTMEPVRTARRLEDPD-GIQHEDGLCRRNVNQQ 334 + Y++ +++ +DIQ+ E +L YT+ P+ T + P +Q+E N+ + Sbjct: 416 DAYLMGDLFNQEEEDIQDTENDLLNANYTLLPLTTDKSGTRPSTTLQYELHKIGSNIKAE 475 Query: 335 ----PLVQLGY 355 P+ ++GY Sbjct: 476 FKTMPIGKIGY 486 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 28.7 bits (61), Expect = 0.98 Identities = 17/70 (24%), Positives = 31/70 (44%), Gaps = 3/70 (4%) Frame = +2 Query: 155 SEGYVVAYGYSENSDDIQNLERELAKTAYTMEPVRTARRLEDPDGIQHEDGLCRRNVN-- 328 SEG + Y + S+ +++L L +T T T + P G+Q + VN Sbjct: 671 SEGAIYLYYENRQSNTLKSLSSTLIRTFSTFHLATTFQDTNLPSGVQLQQKYVMSGVNEI 730 Query: 329 -QQPLVQLGY 355 + P++ + Y Sbjct: 731 FEPPIIHVSY 740 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 27.1 bits (57), Expect = 3.0 Identities = 23/93 (24%), Positives = 49/93 (52%) Frame = +3 Query: 438 SRLVSQGIKNAMSFAYKLWHEGHKTSSKITSRANSNSYSTNR*LSSSANITIKLSNWMLT 617 S +VS + S + L TSS ++ ++S+S S++ SSS++ S+ Sbjct: 213 SSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSI 272 Query: 618 LASTTTA*PGETEKTTPATMSAGNSSLFGKTTT 716 ++S++++ + +T +T+S+ +SS T+T Sbjct: 273 ISSSSSS--SSSPTSTSSTISSSSSSSSSPTST 303 >SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1201 Score = 27.1 bits (57), Expect = 3.0 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = +2 Query: 608 DANVGEYNDRLTWGDGKDYTSHHVSWQLISLWEDNNVIFKILNTE 742 D + E N+R+ G KD + +S ++ +D N FK++N E Sbjct: 929 DIYIDESNERVVKGKSKDNFEYFLSGNTVTRKKD-NACFKMMNFE 972 >SPAC1039.08 |||serine acetyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 26.6 bits (56), Expect = 3.9 Identities = 27/112 (24%), Positives = 49/112 (43%), Gaps = 8/112 (7%) Frame = +3 Query: 249 SRYELPADLKTQTAFSTKMVFADATSINNHLYNLVTGGDYINAVKTVRS-LVDNQG---- 413 S+ L L A V + S+ N Y+++ +N K +RS ++D Sbjct: 44 SKKTLRESLSCVLALPLDHVTGSSESMENWFYSILG----LNDEKIIRSAMIDLDRLYTT 99 Query: 414 SDVCRDVVSRLVSQGIKNAMSF---AYKLWHEGHKTSSKITSRANSNSYSTN 560 + C D++S +S +A+ A+ LW EG KTS+ + + +Y + Sbjct: 100 NPACPDLISAFLSFRGFHALQLYRIAHGLWSEGDKTSAVLVQNWTAVAYGVD 151 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 26.2 bits (55), Expect = 5.2 Identities = 20/62 (32%), Positives = 37/62 (59%) Frame = +3 Query: 510 TSSKITSRANSNSYSTNR*LSSSANITIKLSNWMLTLASTTTA*PGETEKTTPATMSAGN 689 TSS +S ++S+S S++ SSS++ S+++ T++S+T T TP++ S+ Sbjct: 211 TSSTSSSHSSSSSSSSS---SSSSSRPSSSSSFITTMSSSTFI---STVTVTPSSSSSST 264 Query: 690 SS 695 SS Sbjct: 265 SS 266 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 25.8 bits (54), Expect = 6.9 Identities = 12/34 (35%), Positives = 20/34 (58%), Gaps = 1/34 (2%) Frame = -2 Query: 709 VFPKRDELPADMVAGVVFSVSPG-QAVVVLANVS 611 + P ++PAD+ A + S PG + VVV N++ Sbjct: 735 ILPIYSQMPADLQAKIFDSAEPGVRKVVVATNIA 768 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 25.8 bits (54), Expect = 6.9 Identities = 15/47 (31%), Positives = 21/47 (44%) Frame = +2 Query: 74 DSVKTVTISTGPITKRCAADVARIVNASEGYVVAYGYSENSDDIQNL 214 DS + T G I +V RI+N +AYG SD +N+ Sbjct: 154 DSQRAATKDAGAIA---GLNVLRIINEPTAAAIAYGLDAKSDKPKNV 197 >SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 25.8 bits (54), Expect = 6.9 Identities = 15/58 (25%), Positives = 24/58 (41%) Frame = +3 Query: 75 TASKLLPSARGRSLSGALQTSPGLSTRPKATSLHTDILKILTTFKISSVSWQKRLILW 248 +A+K S RG + L T G + RPK +L + + +WQ + W Sbjct: 6 SATKFTFSKRGPCVFRYLSTLEGTTVRPKKNKFLVGLLSAVPIVTFALGTWQVKRREW 63 >SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 25.4 bits (53), Expect = 9.1 Identities = 46/222 (20%), Positives = 93/222 (41%), Gaps = 9/222 (4%) Frame = +3 Query: 6 SKYPTGSPSCLWTTATLPVTSETTASKLLPSA----RGRSLSGALQTSPGLSTRPKATSL 173 S P+ S S L +++ ++S +++S L S+ S +L +S + + SL Sbjct: 39 SVIPSSSSSMLSSSSATAISSSSSSSPLSSSSFTSPASSSFITSLVSSSSQQSSSSSASL 98 Query: 174 HTDILKILTTFKISSV--SWQKRLILWSRYELPADLKTQTAFSTKMVFADA---TSINNH 338 + LT+ +S S + S L A + S+ + + + +S + H Sbjct: 99 TSSSSATLTSSSSASPTSSSSSHALSSSSSSLVASSSSSGMSSSSLSHSSSVPSSSSSYH 158 Query: 339 LYNLVTGGDYINAVKTVRSLVDNQGSDVCRDVVSRLVSQGIKNAMSFAYKLWHEGHKTSS 518 ++ T G +++ ++ S G + V + VS+ + + W+ +S Sbjct: 159 SSSMTTSG--LSSSASIVSSTYRDGPSIITLVSTSYVSEVVTPTTT---NNWNSSSSFTS 213 Query: 519 KITSRANSNSYSTNR*LSSSANITIKLSNWMLTLASTTTA*P 644 +S S+SYS++ L S +N K SN + + + A P Sbjct: 214 STSSTPISSSYSSSGTLPSKSN---KSSNHVGVVVGCSVAIP 252 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 25.4 bits (53), Expect = 9.1 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = +3 Query: 378 VKTVRSLVDNQGSDVCRDVVSRLVSQGIKNAMSFAYKLWH 497 +K R ++++ SD+C DV+S L I A + K+++ Sbjct: 83 IKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFY 122 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,799,376 Number of Sequences: 5004 Number of extensions: 52286 Number of successful extensions: 203 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 191 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 203 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 373338084 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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