Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00280
         (745 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g30630.1 68414.m03746 coatomer protein epsilon subunit family...    65   5e-11
At2g34840.1 68415.m04278 coatomer protein epsilon subunit family...    64   7e-11
At1g58440.1 68414.m06648 squalene monooxygenase, putative / squa...    30   1.9  
At3g29075.1 68416.m03637 glycine-rich protein                          27   9.9  
At2g22475.1 68415.m02666 GRAM domain-containing protein / ABA-re...    27   9.9  

>At1g30630.1 68414.m03746 coatomer protein epsilon subunit family
           protein / COPE family protein similar to SP|O14579
           Coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP) from Homo sapiens, SP|Q60445 from
           Cricetulus griseus; ESTs gb|Z17908, gb|AA728673,
           gb|N96555, gb|H76335, gb|AA712463, gb|W43247, gb|T45611,
           gb|T21160, gb|T14119 and AI100483 come from this gene
          Length = 292

 Score = 64.9 bits (151), Expect = 5e-11
 Identities = 29/74 (39%), Positives = 48/74 (64%)
 Frame = +2

Query: 287 VAKGTELSNEIFLIVAATIYYHEDNYEAALKILHNAESLELRAFTLQCLLAMNRPDLARK 466
           +A  T  +N I  ++A TI+ HE++Y  ALK  H+  +++L A  +Q  + M+R D A K
Sbjct: 98  LADPTVGNNAIIRLIAGTIFMHEEDYNEALKHTHSGGTMDLHALNVQIFIKMHRSDFAEK 157

Query: 467 QLKLLQDIEDDGTL 508
           QL+++Q I++D TL
Sbjct: 158 QLRVMQQIDEDHTL 171



 Score = 58.4 bits (135), Expect = 5e-09
 Identities = 34/93 (36%), Positives = 56/93 (60%), Gaps = 1/93 (1%)
 Frame = +3

Query: 18  DELFDVKNAFYVGNYQQAINEAQSVSPSTPLVALQRDAFLYRSYIAQGNYRIVQQEL-KT 194
           D LF+++N FY+G YQ AIN ++  + S   + ++RD  ++R+YIA G+Y++V  E+ + 
Sbjct: 8   DHLFNLRNHFYLGAYQAAINNSEIPNLSQEDI-VERDCLVHRAYIALGSYQLVISEIDEA 66

Query: 195 ADPMLQPLKSLVDYLLPDANNLPLSLILMLEWL 293
           A   LQ +K L  Y L    N   ++  + EWL
Sbjct: 67  AATPLQAVKLLAMY-LSSPENKESTISSLREWL 98



 Score = 33.5 bits (73), Expect = 0.15
 Identities = 23/77 (29%), Positives = 33/77 (42%)
 Frame = +1

Query: 511 QLAQAWLNLIQGGPGIQDAHYSVMELSEXXXXXXXXXXXXXXXXXXXXXMWEEAEQQLTD 690
           QLA AWLNL  GG  IQ+A+    + SE                      +EEAE  L +
Sbjct: 173 QLASAWLNLAVGGSKIQEAYLIFQDFSEKYPMTSLILNGKAVCCMHMGN-FEEAETLLLE 231

Query: 691 AASRAPQHADLLLGLAL 741
           A ++  +  + L  L +
Sbjct: 232 ALNKDAKDPETLANLVV 248


>At2g34840.1 68415.m04278 coatomer protein epsilon subunit family
           protein / COPE family protein similar to SP|O14579
           Coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP) from Homo sapiens, SP|Q60445 from
           Cricetulus griseus
          Length = 293

 Score = 64.5 bits (150), Expect = 7e-11
 Identities = 36/93 (38%), Positives = 59/93 (63%), Gaps = 1/93 (1%)
 Frame = +3

Query: 18  DELFDVKNAFYVGNYQQAINEAQSVSPSTPLVALQRDAFLYRSYIAQGNYRIVQQEL-KT 194
           D LF+++N FY+G YQ AIN ++ ++  +P  A++RD  ++RSYIA G+Y++V  E+ ++
Sbjct: 9   DHLFNLRNNFYLGAYQTAINNSE-IANLSPENAVERDCLVFRSYIALGSYQLVISEIDES 67

Query: 195 ADPMLQPLKSLVDYLLPDANNLPLSLILMLEWL 293
           A   LQ +K L  Y L    N   ++  + EWL
Sbjct: 68  AATPLQAVKLLAMY-LSTPQNKESTISSLKEWL 99



 Score = 57.6 bits (133), Expect = 8e-09
 Identities = 28/99 (28%), Positives = 55/99 (55%)
 Frame = +2

Query: 212 AIEEFGRLLTT*CQ*SAIVADIDARVAKGTELSNEIFLIVAATIYYHEDNYEAALKILHN 391
           A++     L+T     + ++ +   +A  T  +N+   ++A  I+ HE++Y   LK  H 
Sbjct: 74  AVKLLAMYLSTPQNKESTISSLKEWLADSTIGNNDTLRLIAGIIFMHEEDYNETLKHTHA 133

Query: 392 AESLELRAFTLQCLLAMNRPDLARKQLKLLQDIEDDGTL 508
             +++L A  +Q  + M+R + A KQL+++Q I++D TL
Sbjct: 134 GGTMDLYALNVQIFIKMHRAEYAEKQLRVMQQIDEDHTL 172



 Score = 32.3 bits (70), Expect = 0.35
 Identities = 15/28 (53%), Positives = 18/28 (64%)
 Frame = +1

Query: 511 QLAQAWLNLIQGGPGIQDAHYSVMELSE 594
           QLA AWLNL  GG  IQ+A+    + SE
Sbjct: 174 QLASAWLNLAVGGSKIQEAYLIFEDFSE 201


>At1g58440.1 68414.m06648 squalene monooxygenase, putative /
           squalene epoxidase, putative similar to SP|O65404 (SE
           1,1), SP|O65402 (SE 1,2) 6566341 dbj AB008021.1 AB008021
          Length = 531

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 18/61 (29%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
 Frame = +3

Query: 54  GNYQQAINEAQSVSP-STPLVALQRDAFLYRSYIAQGNYRIVQQELKTADPMLQPLKSLV 230
           GN +   N +   SP  TP   L  DAF  R  +  G   +   ++     +L+PL+ L 
Sbjct: 322 GNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVVLRNLLRPLRDLS 381

Query: 231 D 233
           D
Sbjct: 382 D 382


>At3g29075.1 68416.m03637 glycine-rich protein 
          Length = 294

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 4/48 (8%)
 Frame = +3

Query: 12  DVDELFDVKNAFYVGNYQQAINEAQSVSPST----PLVALQRDAFLYR 143
           DVD+  +     Y G Y   +   +S+ PS     PL +L  DAF Y+
Sbjct: 10  DVDDFTEYDPMPYSGGYDITVTYGRSIPPSDETCYPLSSLSGDAFEYQ 57


>At2g22475.1 68415.m02666 GRAM domain-containing protein /
           ABA-responsive protein-related similar to ABA-responsive
           protein [Hordeum vulgare] GI:4103635; contains Pfam
           profile PF02893: GRAM domain
          Length = 299

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
 Frame = +3

Query: 153 AQGNYRIVQQELKTADPMLQPLKSLVDYLLPDANNLPLSLILMLEWLKVRSC---PMKYS 323
           A+G Y  + ++    DP  Q L S   YL   A   P+  +L +   K+  C   P+ Y 
Sbjct: 169 AEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAG--PVMGVLYISSAKLAYCSDNPLSYK 226

Query: 324 *LWLQQFTIMKIIMKLH 374
                +++  K+++ LH
Sbjct: 227 NGDQTEWSYYKVVIPLH 243


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,248,563
Number of Sequences: 28952
Number of extensions: 305348
Number of successful extensions: 718
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 696
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 716
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)