Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00278
(649 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.04c |||RNase P and RNase MRP subunit p30 |Schizosacchar... 49 7e-07
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 27 2.3
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 4.1
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 26 4.1
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 25 7.1
SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schiz... 25 7.1
SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomy... 25 7.1
SPCC645.13 |||transcription elongation regulator|Schizosaccharom... 25 7.1
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.4
>SPAC3A12.04c |||RNase P and RNase MRP subunit p30
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 235
Score = 48.8 bits (111), Expect = 7e-07
Identities = 30/88 (34%), Positives = 44/88 (50%)
Frame = +2
Query: 263 RIPFKVHRKLYKQAVDRGIFFEIMYSPIIRDSTARKNIISNAHVYHTVGKSKNIILTSGA 442
R+PF + AV R I EI YS +RD + R+N+I+NA + + II+TS
Sbjct: 120 RLPFYLKHTFMGLAVSRDIGIEISYSSGLRDVSNRRNLITNATSLVRATRGRGIIVTSET 179
Query: 443 DNHMHIRSVNDIINLGFLLGLNTTRA*K 526
+ R+ D+INL L +A K
Sbjct: 180 RTPLECRAGFDVINLATFWDLKQDQARK 207
Score = 34.7 bits (76), Expect = 0.012
Identities = 20/66 (30%), Positives = 36/66 (54%)
Frame = +3
Query: 75 QTKLNILQRITIEFSDSGIVHKLNRSENIKKYDIIAVIPKTLQAFQYACSSMDIDIISFE 254
+ K+ I RIT+ +S +K+ S K++DI+A+ P + Q CS ++ DI+S +
Sbjct: 59 EQKIKIYSRITLTI-ESMPQNKV-LSNVTKEFDILAIRPIGDRLLQQTCSDLEFDILSID 116
Query: 255 LKEEYP 272
+ P
Sbjct: 117 FTQRLP 122
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 2.3
Identities = 13/48 (27%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = -1
Query: 556 KSIFVYCCVQLLSS-RCIEAKQKSQIYNIIYTTDMHVIVSSTCEDYIL 416
++++V+ V+ S RC+ S I N+++ ++H ++ ST IL
Sbjct: 438 ETLYVHIYVKSSKSWRCMSQTHVSSISNLVWLNELHQLLFSTSNGAIL 485
>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 268
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = -3
Query: 473 HLHYGYACDCQLHL*GLYSLISPQCGKHGHCLLYSFGLWNL***GSTLFQKIY 315
H + Y+C C+ SL P C +H C+ Y F GS+L++K Y
Sbjct: 39 HDQFDYSCICRWMD---QSLTCPICKRHVDCVFYGFH-------GSSLYKKWY 81
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/51 (27%), Positives = 26/51 (50%)
Frame = +3
Query: 57 PKDNNFQTKLNILQRITIEFSDSGIVHKLNRSENIKKYDIIAVIPKTLQAF 209
PK N TKL R+ E ++S + K+ ++N++K + P ++ F
Sbjct: 200 PKSNAQLTKLEDEARLKAENAESDMHSKIENAQNVQKQLLCIHRPNYIKQF 250
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 25.4 bits (53), Expect = 7.1
Identities = 7/23 (30%), Positives = 17/23 (73%)
Frame = -1
Query: 496 QKSQIYNIIYTTDMHVIVSSTCE 428
++S+++N+++T ++H V CE
Sbjct: 3653 RRSELFNVLFTYNLHPSVIKQCE 3675
>SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 451
Score = 25.4 bits (53), Expect = 7.1
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -2
Query: 363 AVESLIIGEYIISKNIPLSTACL*SLRCTLK 271
A+E+L+I E ++S ++P L + TLK
Sbjct: 259 AIETLLINEAVLSSHLPKIAETLTEAKVTLK 289
>SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 764
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/53 (26%), Positives = 27/53 (50%)
Frame = +2
Query: 278 VHRKLYKQAVDRGIFFEIMYSPIIRDSTARKNIISNAHVYHTVGKSKNIILTS 436
V+ +L K+ + G + + PI+ ++ + S Y T+GK +ILT+
Sbjct: 195 VYVELIKKLTEAGAEYIQIDEPILTLDLPQEILASYKEAYETLGKIGKLILTT 247
>SPCC645.13 |||transcription elongation
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 25.4 bits (53), Expect = 7.1
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +3
Query: 21 EIKDFVPPPMDLPKDNNFQ 77
E+ D +P +DLPKD NFQ
Sbjct: 600 ELLDLLPK-VDLPKDRNFQ 617
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -3
Query: 338 STLFQKI-YPCLQLVYKVYDVL*RVFFLQFET 246
S FQ++ +PC L YKVY V F+ ++ T
Sbjct: 15 SDTFQEVQHPCSILKYKVYFVTTDTFYCKYLT 46
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,609,274
Number of Sequences: 5004
Number of extensions: 55872
Number of successful extensions: 153
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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