Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00272
         (754 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC24C9.08 |||vacuolar carboxypeptidase |Schizosaccharomyces po...    56   7e-09
SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos...    29   0.94 
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo...    28   1.2  
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy...    28   1.6  
SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo...    27   3.8  
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...    26   5.0  
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...    26   5.0  
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M...    25   8.8  

>SPAC24C9.08 |||vacuolar carboxypeptidase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 596

 Score = 55.6 bits (128), Expect = 7e-09
 Identities = 32/85 (37%), Positives = 47/85 (55%), Gaps = 3/85 (3%)
 Frame = +2

Query: 257 LPSILLNSHMDVVPVFENS---WKHPPFAAEIEDNVIYARGVQDMKSVGIQYIEAVRRLK 427
           L  ++L  H DVVPV + S   W  PPF+A   +  +Y+RG  D K+  +  +EA+  L 
Sbjct: 189 LKPLVLMGHQDVVPVNQASLDRWYFPPFSATYHNGHVYSRGAADDKNSVVAILEALEILA 248

Query: 428 ETGVKLKRTVHLSFVPDEEIGGDTG 502
            +  K ++TV  SF  DEE+ G  G
Sbjct: 249 ISDYKPEQTVIASFGFDEEVSGYRG 273


>SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 891

 Score = 28.7 bits (61), Expect = 0.94
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
 Frame = -3

Query: 233 HYQRRLLWQRFDHLHL*SNLFSFIFEEV--DAFIVVN 129
           HY R++LW    + H  + +FSF  EE   D ++ VN
Sbjct: 238 HYCRKILWPSLHYQH--NEIFSFFHEESNWDDYVAVN 272


>SPAPB8E5.07c |||ribosome biogenesis protein
           Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1163

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
 Frame = +1

Query: 16  RFAL-FPQCSEYIQQVQSRLTPASRPYR-TILEYVQYIR 126
           RF L +P+C + I  +  +L P S PY    LE + Y+R
Sbjct: 378 RFRLAYPECFQIISSLCDKLGPHSDPYLIPALEVIDYLR 416


>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 603

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 13/46 (28%), Positives = 21/46 (45%)
 Frame = +3

Query: 45  IHTTSSVKIDPSVSTLQNYLRIRSVHPNVDYNECINFLKNEAEKIG 182
           +HT  S  ++ S+S L N   I  VH N  +   I+       ++G
Sbjct: 269 LHTVDSTNVNGSLSPLSNSSSINQVHQNETHGSTISVPNPNLSQMG 314


>SPBC8D2.05c |sfi1||spindle pole body protein
           Sfi1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 840

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 18/64 (28%), Positives = 28/64 (43%), Gaps = 4/64 (6%)
 Frame = +1

Query: 28  FPQCSEYIQQVQSRLTPASRPYRTILEYVQYIRTLTTMNASTSSKMKLKR----LDYKCK 195
           FP+  + ++ +QSR+  A   YR IL      R           K++ ++    LD   K
Sbjct: 207 FPRWVQKLRNIQSRIYSAGEAYRLILIGQMVNRWRQKTRERLHQKLRFQKNAFLLDVLLK 266

Query: 196 WSNL 207
           WS L
Sbjct: 267 WSAL 270


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 12/42 (28%), Positives = 18/42 (42%)
 Frame = +3

Query: 591 PGLQWRKNNLACENHLSRKIRSRFPSAPRQLRRKVAVHNRQV 716
           P L+   +   CE  + RK  +R P      R+K   H  Q+
Sbjct: 80  PSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQL 121


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 12/42 (28%), Positives = 18/42 (42%)
 Frame = +3

Query: 591 PGLQWRKNNLACENHLSRKIRSRFPSAPRQLRRKVAVHNRQV 716
           P L+   +   CE  + RK  +R P      R+K   H  Q+
Sbjct: 80  PSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQL 121


>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1052

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 17/63 (26%), Positives = 28/63 (44%)
 Frame = +1

Query: 565 GVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPDNCGEKLRYIIDKFMDLRQESVK 744
           G AS ++D  +  GE  +  + +    K   G + L     E+++  I     L   S+K
Sbjct: 48  GKASSSEDVSISTGEEEMRTLLVASAVKDAKGWVYLSGKDVEEIKQKIKNLQGLLGVSIK 107

Query: 745 KLA 753
           KLA
Sbjct: 108 KLA 110


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,198,713
Number of Sequences: 5004
Number of extensions: 68302
Number of successful extensions: 201
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)